Human microbiota modulation via QseC sensor kinase mediated in the Escherichia coli O104:H4 outbreak strain infection in microbiome model

Abstract

Background The intestinal microbiota plays a crucial role in human health, adjusting its composition and the microbial metabolites protects the gut against invading microorganisms. Enteroaggregative E. coli (EAEC) is an important diarrheagenic pathogen, which may cause acute or persistent diarrhea (≥14 days). The outbreak strain has the potent Shiga toxin, forms a dense biofilm and communicate via QseBC two-component system regulating the expression of many important virulence factors. Results Herein, we investigated the QseC histidine sensor kinase role in the microbiota shift during O104:H4 C227–11 infection in the colonic model SHIME® (Simulator of the Human Intestinal Microbial Ecosystem) and in vivo mice model. The microbiota imbalance caused by C227–11 infection affected ỿ-Proteobacteria and Lactobacillus spp. predominance, with direct alteration in intestinal metabolites driven by microbiota change, such as Short-chain fatty acids (SCFA). However, in the absence of QseC sensor kinase, the microbiota recovery was delayed on day 3 p.i., with change in the intestinal production of SCFA, like an increase in acetate production. The higher predominance of Lactobacillus spp. in the microbiota and significant augmented qseC gene expression levels were also observed during C227–11 mice infection upon intestinal depletion. Novel insights during pathogenic bacteria infection with the intestinal microbiota were observed. The QseC kinase sensor seems to have a role in the microbiota shift during the infectious process by Shiga toxin-producing EAEC C227–11. Conclusions The QseC role in C227–11 infection helps to unravel the intestine microbiota modulation and its metabolites during SHIME® and in vivo models, besides they contribute to elucidate bacterial intestinal pathogenesis and the microbiota relationships.

A Novel Adult Murine Model of Typical Enteroaggregative Escherichia coli Infection Reveals Microbiota Dysbiosis, Mucus Secretion, and AAF/II-Mediated Expression and Localization of β-Catenin and Expression of MUC1 in Ileum

“…Of interest, class Gammaproteobacteria increases in the bacteriota of antibiotic-treated mice ( Sekirov et al., 2008 ; Barreto et al., 2020 ), and animals are more prone to Salmonella enterica serovar Typhimurium infection ( Sekirov et al., 2008 ). Similar to our observations, mice infected with the tEAEC-STEC O104:H4 C227-11 strain had an imbalance of bacteriota, manifested as an increase in γ-Proteobacteria and Lactobacillus spp., with direct alteration in intestinal metabolites driven by microbiota changes ( Machado Ribeiro et al., 2021 ).…”

Metagenome Analysis of the Bacterial Characteristics in Invasive Klebsiella Pneumoniae Liver Abscesses

“…Recent studies on the role of intestinal microbiota in pathogenic gastrointestinal infections allude that these organisms participate in the upregulation or downregulation of bacterial pathogen virulence (Menezes-Garcia et al, 2020;Wang et al, 2020;Cho et al, 2021;Machado Ribeiro et al, 2021; Shil The clustering correlation heatmap showed that there were differences in the abundance of many species of Klebsiella between KPLA and IKPLA (A). Furthermore, comparative analysis was performed for four members of the Klebsiella pneumoniae species complex (KpSC).…”

Quorum sensing in human gut and food microbiomes: Significance and potential for therapeutic targeting

“…Accurate and rapid detection, characterisation and quantification of QSM can enhance our understanding of QS in various environments, as well as providing a real-time readout of microbial physiology within a given microbiome. Overview of representative bacterial quorum sensing systems: Escherichia coli cell illustrates an Autoinducer 3 (AI-3) Quorum Sensing (QS) system which regulates the locus of enterocyte effacement (LEE) pathogenicity island (Walters and Sperandio, 2006;Machado Ribeiro et al, 2021); an Autoinducing Peptide (AIP) system in Lactococcus lactis is presented, in which nisin has a dual role as a Quorum Sensing Molecule (QSM) and antimicrobial peptide (Kleerebezem, 2004); Vibrio fischeri cell illustrates the control of bioluminescence through an Acylated Homoserine Lactone (AHL) system also referred to as Autoinducer 1 (AI-1; Nealson, Platt and Woodland Hastings, 1970); Autoinducer 2 (AI-2) signalling is presented at the intersection due to its role in species-and genera-agnostic communications. The AI-2 biosynthetic pathway is highlighted in yellow alongside the Lsr/Lux pathways which provide for its internalisation/detection in various genera (Pereira, Thompson and Xavier, 2013).…”