Human microvascular endothelial cells express receptors for platelet-derived growth factor.

Beitz, Julie; Kim, In-Soon; Calabresi, Paul; Frackelton, A. Raymond

doi:10.1073/pnas.88.5.2021

Cited by 126 publications

(68 citation statements)

References 54 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This, in addition to their finding that incubation with PDGF had no effect on Achinduced relaxation of intact rat aortic strips, led them to the conclusion that PDGF exerts its vasoactive effects in an endothelium-independent manner. This conclusion is in agreement with studies ofcultured cells that show macrovascular endothelial cells do not respond to PDGF, presumably because they lack PDGF receptors (10)(11)(12)(13)(14)(15)(16)(17)(18). The observation of endothelium-independent responses to PDGF-AB and AA in the present study lends support to this view.…”

Section: Resultssupporting

confidence: 82%

See 1 more Smart Citation

Platelet-derived growth factor receptors on macrovascular endothelial cells mediate relaxation via nitric oxide in rat aorta.

Cunningham¹,

Brecher²,

Cohen³

1992

J. Clin. Invest.

View full text Add to dashboard Cite

The effects ofplatelet-derived growth factor (PDGF) were studied in isolated rings of rat aorta contracted submaximally to phenylephrine. The BB isoform of PDGF elicited relaxation in rings with endothelium and further contraction in rings without endothelium. Both the endothelium-dependent relaxation and endothelium-independent contraction occurred at concentrations known to induce PDGF receptor-mediated responses in cultured cells. Furthermore, the relaxation was isoform specific. This conclusion is supported by the unique ability of PDGF-BB to induce endothelium-dependent relaxations, as well as by studies showing isoform specific, concentration-dependent desensitization of PDGF-BB relaxation. The relaxation induced by PDGF-BB was prevented by Nw-nitro-L-arginine. It was also observed that endothelium-independent contractions to the AB and AA isoforms of PDGF were less than those to PDGF-BB. Contrary to the widely held view that PDGF receptors are not present on the endothelium of macrovessels, these studies provide evidence for an endothelium-dependent, nitric oxide mediated relaxation of rat aorta caused by PDGF via PDGF,8,

show abstract

Section: Resultssupporting

confidence: 82%

“…However, it is a widely held view that PDGF receptors are not present on the endothelium of large vessels (10)(11)(12)(13)(14)(15)(16)(17)(18). Also, it is not known if the interaction ofPDGF with endothelial cell receptors is capable of eliciting changes in vascular tone.…”

Section: Introductionmentioning

confidence: 99%

Platelet-derived growth factor receptors on macrovascular endothelial cells mediate relaxation via nitric oxide in rat aorta.

Cunningham¹,

Brecher²,

Cohen³

1992

J. Clin. Invest.

View full text Add to dashboard Cite

show abstract

“…2 A). Cell counts also revealed a dramatic associated increase in cell numbers 7 and 10 d after implantation (7 d 6.7±2 x 106, P < 0.001) (Fig. 2 B).…”

Section: Resultsmentioning

confidence: 89%

“…Key words: angiogenesis * extracellular matrix * cell proliferation * fibroblasts * gene therapy Several growth factors have been identified which stimulate cell proliferation and extracellular matrix production. Plateletderived growth factor (PDGF) induces fibroblasts and smooth muscle cells to migrate and proliferate (1)(2)(3)(4), and it is synthesized by, and stimulates mesenchymally derived cells and endothelial cells (5)(6)(7)(8). In addition, PDGF is a potent chemotactic agent for monocytes (9,10).…”

Section: Introductionmentioning

confidence: 99%

De novo generation of permanent neovascularized soft tissue appendages by platelet-derived growth factor.

et al. 1994

View full text Add to dashboard Cite

IntroductionTreatment of wounds with pharmacologic doses of the BB homodimeric form of recombinant PDGF (rPDGF-BB) induces the recruitment, activation, and proliferation of mesenchymal cells, resulting in the deposition of provisional, and subsequently collagen-containing extracellular matrix.In preliminary experiments with an in vitro growth chamber model in the rat consisting of a silicone shell containing a dissected femoral vascular bundle, we found that rPDGF-

show abstract

“…Cells were stimulated with VEGF (100 ng/ml) or SPP (1 M) for 10 min, and reactions were stopped with lysis buffer as described above. Forskolin (10 M) was used as a positive control (see and microvascular endothelial cells from several sources do express an active PDGFR (53)(54)(55).…”

Section: Su1498 Has No Effect On Nuclear Localization Of Erk1/2-mentioning

confidence: 99%

SU1498, an Inhibitor of Vascular Endothelial Growth Factor Receptor 2, Causes Accumulation of Phosphorylated ERK Kinases and Inhibits Their Activity in Vivo and in Vitro

Boguslawski

McGlynn

Harvey

et al. 2004

Journal of Biological Chemistry

View full text Add to dashboard Cite

SU1498, an inhibitor of vascular endothelial growth factor receptor 2, has been used successfully to study the physiological manifestations of receptor functions. Here we report that in addition to its anti-receptor activity, SU1498 stimulates accumulation of phosphorylated ERKs in human umbilical vein endothelial cells and in human aortic endothelial cells in a manner that is dependent on the functioning of the upstream components of the MAPK pathway, B-Raf, and MEK kinases. The enhanced accumulation of phospho-ERKs is observed only in cells that have been stimulated with sphingosine 1-phosphate or protein growth factors; SU1498 by itself is ineffective. We show that the inhibitor acts by blocking the kinase activity of phospho-ERK both in a direct assay and in immunoprecipitates from cells treated with the compound. The data reveal a novel and unique way in which MAPK signaling pathway may be blocked in human endothelial cells.

show abstract

Human microvascular endothelial cells express receptors for platelet-derived growth factor.

Abstract: Endothelial

Cited by 126 publications

References 54 publications

Platelet-derived growth factor receptors on macrovascular endothelial cells mediate relaxation via nitric oxide in rat aorta.

Platelet-derived growth factor receptors on macrovascular endothelial cells mediate relaxation via nitric oxide in rat aorta.

De novo generation of permanent neovascularized soft tissue appendages by platelet-derived growth factor.

SU1498, an Inhibitor of Vascular Endothelial Growth Factor Receptor 2, Causes Accumulation of Phosphorylated ERK Kinases and Inhibits Their Activity in Vivo and in Vitro

Contact Info

Product

Resources

About