Human milk and mucosal lacto- and galacto-N-biose synthesis by transgalactosylation and their prebiotic potential in Lactobacillus species

Bidart, Gonzalo N.; Rodrı́guez-Dı́az, Jesús; Palomino‐Schätzlein, Martina; Monedero, Vicente; Yebra, Marı́a J.

doi:10.1007/s00253-016-7882-0

Cited by 16 publications

(21 citation statements)

References 50 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The disaccharides LNB, GNB, 3FN and 6FN were synthesized by transglycosylation reactions as previously described 20 , 21 , and they were tested independently in fermentation assays, using total microbiota isolated from stool samples of breastfed infants. The addition of any of those oligosaccharides decreased the culture pH (Table 1 ).…”

Section: Resultsmentioning

confidence: 99%

“…animalis and Bifidobacterium pseudolongum 22 . In addition, we have seen that LNB and GNB are also fermented by pure cultures of Lactobacillus species 20 . In this work, we have investigated, in batch cultures using total microbiota isolated from stool samples of breastfed infants, the effects of LNB, GNB, 3FN and 6FN on infant microbiota composition and subsequent short chain fatty acids production.…”

Section: Introductionmentioning

confidence: 85%

“…3FN forms part of HMOs 2 and 6FN of N -glycosylated proteins, including mucins 19 . We have also produced LNB and galacto- N -biose (GNB), the core type-1 sugar from mucins, using the transgalactosylation activity of the β-galactosidase GnbG 20 . The synthesized fucosyl- N -GlcNAc disaccharides are fermented in vitro by pure cultures of L. casei , L. rhamnosus , B. breve and B. pseudocatenulatum species 21 .…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Human milk and mucosa-associated disaccharides impact on cultured infant fecal microbiota

Rubio‐del‐Campo

Alcántara

Collado

et al. 2020

Sci Rep

Self Cite

View full text Add to dashboard Cite

Human milk oligosaccharides (HMOs) are a mixture of structurally diverse carbohydrates that contribute to shape a healthy gut microbiota composition. The great diversity of the HMOs structures does not allow the attribution of specific prebiotic characteristics to single milk oligosaccharides. We analyze here the utilization of four disaccharides, lacto- N -biose (LNB), galacto- N -biose (GNB), fucosyl-α1,3-GlcNAc (3FN) and fucosyl-α1,6-GlcNAc (6FN), that form part of HMOs and glycoprotein structures, by the infant fecal microbiota. LNB significantly increased the total levels of bifidobacteria and the species Bifidobacterium breve and Bifidobacterium bifidum . The Lactobacillus genus levels were increased by 3FN fermentation and B. breve by GNB and 3FN. There was a significant reduction of Blautia coccoides group with LNB and 3FN. In addition, 6FN significantly reduced the levels of Enterobacteriaceae family members. Significantly higher concentrations of lactate, formate and acetate were produced in cultures containing either LNB or GNB in comparison with control cultures. Additionally, after fermentation of the oligosaccharides by the fecal microbiota, several Bifidobacterium strains were isolated and identified. The results presented here indicated that each, LNB, GNB and 3FN disaccharide, might have a specific beneficial effect in the infant gut microbiota and they are potential prebiotics for application in infant foods.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 85%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Human milk and mucosa-associated disaccharides impact on cultured infant fecal microbiota

Rubio‐del‐Campo

Alcántara

Collado

et al. 2020

Sci Rep

Self Cite

View full text Add to dashboard Cite

show abstract

“…The gnb gene cluster is present in strains of the closely related species L. casei–paracasei–rhamnosus . These disaccharides are also metabolized by other species of intestinal lactobacilli ( L. gasseri and L. johnsonii ) although homologs of the gnb genes are not detected in their chromosomes, suggesting that as yet undiscovered pathways for the use of these mucosal-derived disaccharides are active in these bacteria ( Bidart et al, 2014 , 2017 ).…”

Section: Utilization Of Hmos By Lactobacillimentioning

confidence: 99%

Utilization of Host-Derived Glycans by Intestinal Lactobacillus and Bifidobacterium Species

2018

Self Cite

View full text Add to dashboard Cite

Members of the genus Lactobacillus are commonly found at the gastrointestinal tract and other mucosal surfaces of humans. This genus includes various species with a great number of potentially probiotic bacteria. Other often-used probiotic species belong to Bifidobacterium, a genus almost exclusively associated with the gut. As probiotics must survive and be metabolically active at their target sites, namely host mucosal surfaces, consumption of host-produced glycans is a key factor for their survival and activity. The ability to metabolize glycans such as human milk oligosaccharides (HMOs), glycosaminoglycans and the glycan moieties of glycoproteins and glycolipids found at the mucosal surfaces grants a competitive advantage to lactobacilli and bifidobacteria. The analyses of the great number of sequenced genomes from these bacteria have revealed that many of them encode a wide assortment of genes involved in the metabolism and transport of carbohydrates, including several glycoside hydrolases required for metabolizing the carbohydrate moieties of mucins and HMOs. Here, the current knowledge on the genetic mechanisms, known catabolic pathways and biochemical properties of enzymes involved in the utilization of host-produced glycans by lactobacilli and bifidobacteria will be summarized.

show abstract

“…Nuclear magnetic resonance spectroscopy. Samples for nuclear magnetic resonance (NMR) spectroscopy were prepared as previously described (66). NMR spectra were also recorded as previously indicated (66), with some modifications.…”

Section: Methodsmentioning

confidence: 99%

Unique Microbial Catabolic Pathway for the Human Core N -Glycan Constituent Fucosyl-α-1,6- N -Acetylglucosamine-Asparagine

Becerra

Rodrı́guez-Dı́az

Gozalbo-Rovira

et al. 2020

mBio

Self Cite

View full text Add to dashboard Cite

The survival of commensal bacteria in the human gut partially depends on their ability to metabolize host-derived molecules. The use of the glycosidic moiety of N-glycoproteins by bacteria has been reported, but the role of N-glycopeptides or glycoamino acids as the substrates for bacterial growth has not been evaluated. We have identified in Lactobacillus casei strain BL23 a gene cluster (alf-2) involved in the catabolism of the glycoamino acid fucosyl-α-1,6-N-GlcNAc-Asn (6′FN-Asn), a constituent of the core-fucosylated structures of mammalian N-glycoproteins. The cluster consists of the genes alfHC, encoding a major facilitator superfamily (MFS) permease and the α-l-fucosidase AlfC, and the divergently oriented asdA (aspartate 4-decarboxylase), alfR2 (transcriptional regulator), pepV (peptidase), asnA2 (glycosyl-asparaginase), and sugK (sugar kinase) genes. Knockout mutants showed that alfH, alfC, asdA, asnA2, and sugK are necessary for efficient 6′FN-Asn utilization. The alf-2 genes are induced by 6′FN-Asn, but not by its glycan moiety, via the AlfR2 regulator. The constitutive expression of alf-2 genes in an alfR2 strain allowed the metabolism of a variety of 6′-fucosyl-glycans. However, GlcNAc-Asn did not support growth in this mutant background, indicating that the presence of a 6′-fucose moiety is crucial for substrate transport via AlfH. Within bacteria, 6′FN-Asn is defucosylated by AlfC, generating GlcNAc-Asn. This glycoamino acid is processed by the glycosylasparaginase AsnA2. GlcNAc-Asn hydrolysis generates aspartate and GlcNAc, which is used as a fermentable source by L. casei. These data establish the existence in a commensal bacterial species of an exclusive metabolic pathway likely to scavenge human milk and mucosal fucosylated N-glycopeptides in the gastrointestinal tract. IMPORTANCE The gastrointestinal tract accommodates more than 1014 microorganisms that have an enormous impact on human health. The mechanisms enabling commensal bacteria and administered probiotics to colonize the gut remain largely unknown. The ability to utilize host-derived carbon and energy resources available at the mucosal surfaces may provide these bacteria with a competitive advantage in the gut. Here, we have identified in the commensal species Lactobacillus casei a novel metabolic pathway for the utilization of the glycoamino acid fucosyl-α-1,6-N-GlcNAc-Asn, which is present in the core-fucosylated N-glycoproteins from mammalians. These results give insight into the molecular interactions between the host and commensal/probiotic bacteria and may help to devise new strategies to restore gut microbiota homeostasis in diseases associated with dysbiotic microbiota.

show abstract

Human milk and mucosal lacto- and galacto-N-biose synthesis by transgalactosylation and their prebiotic potential in Lactobacillus species

Cited by 16 publications

References 50 publications

Human milk and mucosa-associated disaccharides impact on cultured infant fecal microbiota

Human milk and mucosa-associated disaccharides impact on cultured infant fecal microbiota

Utilization of Host-Derived Glycans by Intestinal Lactobacillus and Bifidobacterium Species

Unique Microbial Catabolic Pathway for the Human Core N -Glycan Constituent Fucosyl-α-1,6- N -Acetylglucosamine-Asparagine

Contact Info

Product

Resources

About