Human milk pasteurisation reduces pre-lipolysis but not digestive lipolysis and moderately decreases intestinal lipid uptake in a combination of preterm infant in vitro models

Vincent, Marine; Ménard, Olivia; Etienne, Julie; Ossemond, Jordane; Durand, Annie; Buffin, Rachel; Loizon, Emmanuelle; Meugnier, Emmanuelle; Deglaire, Amélie; Dupont, Didier; Picaud, Jean‐Charles; Knibbe, Carole; Michalski, Marie‐Caroline; Penhoat, Armelle

doi:10.1016/j.foodchem.2020.126927

Cited by 19 publications

(16 citation statements)

References 38 publications

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“…Given that pasteurization, prior the administration, of mother’s own milk is not recommended [ 59 ], also potential pathogenic microorganisms are normally conveyed to preterm infants fed with fresh HM. Nevertheless, breastfeeding is recommended because the potential benefits of breast milk outweigh the possible negative effects: only few articles reported that neonatal infection was caused by the ingestion of contaminated HM [ 60 ].…”

Section: Discussionmentioning

confidence: 99%

Inoculation of mother’s own milk could personalize pasteurized donor human milk used for feeding preterm infants

et al. 2021

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Background Human milk is a vehicle for bioactive compounds and beneficial bacteria which promote the establishment of a healthy gut microbiome of newborns, especially of preterm infants. Pasteurized donor human milk (PDHM) is the second-best option when preterm mother’s own milk is unavailable. Since pasteurization affect the microbiological quality of donor milk, PDHM was inoculated with different preterm milk samples and then incubated, in order to evaluate the effect in terms of bacterial growth, human milk microbiome and proteolytic phenomena. Methods In an in-vitro study PDHM was inoculated at 10% v/v using ten preterm milk samples. Microbiological, metataxonomic and peptidomic analyses, on preterm milk samples at the baseline (T0), on PDHM and on inoculated milk (IM) samples at T0, after 2 h (T1) and 4 h (T2) of incubation at 37 °C, were conducted. Results IM samples at T2 showed a Total Bacterial Count not significantly different (p > 0.01) compared to preterm milk samples. At T2 lactic acid bacteria level was restored in all IM. After inoculation, metataxonomic analysis in IM samples showed that Proteobacteria remained the predominant phylum while Firmicutes moved from 3% at T1 to 9.4% at T2. Peptidomic profile of IM resembled that of PDHM, incubated for the same time, in terms of number and type of peptides. Conclusion The study demonstrated that inoculation of PDHM with mother’s own milk could restore bacterial growth and personalize human milk microbiome in PDHM. This effect could be beneficial because of the presence of maternal probiotic bacteria which make PDHM more similar to mother’s own milk.

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Section: Discussionmentioning

confidence: 99%

Inoculation of mother’s own milk could personalize pasteurized donor human milk used for feeding preterm infants

et al. 2021

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“…We then studied the impact of knocking out MTTP or SAR1B on the expression of different genes involved in lipid metabolism [ 19 ] and oxidative stress management. We explored the expressions of genes involved in fatty acid transport ( FATP4 , FABP2 ), intracellular lipid re-synthesis ( ACAT2 , DGAT1 , DGAT2 , MGAT2 ), lipid droplet ( PLIN2 ) or chylomicron ( APOB ) formation, cholesterol metabolism ( ABCA1 , ABCG8 , NPC1L1 , SCARB1 , PGP ) and oxidative stress management ( CAT , SOD1 ).…”

Section: Discussionmentioning

confidence: 99%

“…All images were collected under light microscopy (Olympus BX63, 60 × 10). Intracellular lipid droplets were quantified as described by Vincent et al [ 19 ] after acquiring 20 images per slide by standard light microscopy. Oil Red O-stained cell images were thresholded for the lipid droplet signal using the freely available imaging software ImageJ.…”

Section: Methodsmentioning

confidence: 99%

Validation of Knock-Out Caco-2 TC7 Cells as Models of Enterocytes of Patients with Familial Genetic Hypobetalipoproteinemias

et al. 2023

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Abetalipoproteinemia (FHBL-SD1) and chylomicron retention disease (FHBL-SD3) are rare recessive disorders of lipoprotein metabolism due to mutations in MTTP and SAR1B genes, respectively, which lead to defective chylomicron formation and secretion. This results in lipid and fat-soluble vitamin malabsorption, which induces severe neuro-ophthalmic complications. Currently, treatment combines a low-fat diet with high-dose vitamin A and E supplementation but still fails in normalizing serum vitamin E levels and providing complete ophthalmic protection. To explore these persistent complications, we developed two knock-out cell models of FHBL-SD1 and FHBL-SD3 using the CRISPR/Cas9 technique in Caco-2/TC7 cells. DNA sequencing, RNA quantification and Western blotting confirmed the introduction of mutations with protein knock-out in four clones associated with i) impaired lipid droplet formation and ii) defective triglyceride (−57.0 ± 2.6% to −83.9 ± 1.6%) and cholesterol (−35.3 ± 4.4% to −60.6 ± 3.5%) secretion. A significant decrease in α-tocopherol secretion was also observed in these clones (−41.5 ± 3.7% to −97.2 ± 2.8%), even with the pharmaceutical forms of vitamin E: tocopherol-acetate and tocofersolan (α-tocopheryl polyethylene glycol succinate 1000). MTTP silencing led to a more severe phenotype than SAR1B silencing, which is consistent with clinical observations. Our cellular models thus provide an efficient tool to experiment with therapeutic strategies and will allow progress in understanding the mechanisms involved in lipid metabolism.

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“…By contrast, bile salt-stimulated lipase in DHM is known to be destroyed by Holder pasteurisation [ 56 , 57 ]. Reduced lipid hydrolysis might thus be a factor in the reduced overall growth inhibition observed in Holder-pasteurised DHM and in Holder-pasteurised FD-DHM.…”

Section: Discussionmentioning

confidence: 99%

The Effects of Thermal Pasteurisation, Freeze-Drying, and Gamma-Irradiation on the Antibacterial Properties of Donor Human Milk

Blackshaw

Valtchev

et al. 2021

Foods

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The most common pasteurisation method used by human milk banks is Holder pasteurisation. This involves thermal processing, which can denature important proteins and can potentially reduce the natural antimicrobial properties found in human milk. This study assesses the application of a hybrid method comprised of freeze-drying followed by low-dose gamma-irradiation for nonthermal donor human milk pasteurisation. Freeze-drying donor human milk followed by gamma-irradiation at 2 kGy was as efficient as Holder pasteurisation in the reduction of bacterial inoculants of Staphylococcus aureus (106 cfu/mL) and Salmonella typhimurium (106 cfu/mL) in growth inhibition assays. These assays also demonstrated that human milk naturally inhibits the growth of bacterial inoculants S. aureus, S. typhimurium, and Escherichia coli. Freeze drying (without gamma-irradiation) did not significantly reduce this natural growth inhibition. By contrast, Holder pasteurisation significantly reduced the milk’s natural antimicrobial effect on S. aureus growth after 6 h (−19.8% p = 0.01). Freeze-dried and then gamma-irradiated donor human milk showed a strong antimicrobial effect across a dose range of 2–50 kGy, with only a minimal growth of S. aureus observed after 6 h incubation. Thus, a hybrid method of freeze-drying followed by 2 kGy of gamma-irradiation preserves antimicrobial properties and enables bulk pasteurisation within sealed packaging of powderised donor human milk. This work forwards a goal of increasing shelf life and simplifying storage and transportation, while also preserving functionality and antimicrobial properties.

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Human milk pasteurisation reduces pre-lipolysis but not digestive lipolysis and moderately decreases intestinal lipid uptake in a combination of preterm infant in vitro models

Cited by 19 publications

References 38 publications

Inoculation of mother’s own milk could personalize pasteurized donor human milk used for feeding preterm infants

Inoculation of mother’s own milk could personalize pasteurized donor human milk used for feeding preterm infants

Validation of Knock-Out Caco-2 TC7 Cells as Models of Enterocytes of Patients with Familial Genetic Hypobetalipoproteinemias

The Effects of Thermal Pasteurisation, Freeze-Drying, and Gamma-Irradiation on the Antibacterial Properties of Donor Human Milk

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