Human Mn superoxide dismutase cDNA sequence

Abstract

Human Mn superoxide dismutase (MnSOD) encoded by chromosome 6 is a mitochondrial matrix enzyme positioned to scavenge oxygen radicals produced by the extensive oxidation-reduction and electron transport reactions undergoing in that organelle. cDNA clones containing the entire coding region for human MnSOD were isolated from a T-lymphocyte cDNA library in A gt1O. The cDNA contains a 666 bp coding region followed by a 3' untranslated region which lacks the AATAAA polyadenylation signal. The predicted amino acid sequence is in accordance with the published amino acid sequence of human liver MnSOD (1) with the following exceptions: Glu instead of Gln at positions 42, 88 and 109 and an additional Gly-Trp after amino acids 123. The deduced protein sequence extends 24 amino acids upstream from the N-terminal Lys of human MnSOD, suggesting a pre-peptide. Hence human MnSOD is composed of 222 amino acids, 24 of which are removed during processing and maturation of the enzyme.

Importance of mitochondrial superoxide dismutase expression in insulin-producing cells for the toxicity of reactive oxygen species and proinflammatory cytokines

“…The cDNA for human mitochondrial MnSOD [15] was subcloned into the pcDNA3 expression vector in sense (MnSODsense) or antisense orientation (MnSODantisense) by standard molecular biology techniques [11]. RINm5F cells were stably transfected with the vector and positive clones were selected through resistance against G418 (250 μg/ml).…”

Improvement of the Mitochondrial Antioxidant Defense Status Prevents Cytokine-Induced Nuclear Factor-κB Activation in Insulin-Producing Cells

“…RINm5F tissue culture cells overexpressing Cat, Gpx, and Cu/ZnSOD were generated through stable transfection as described elsewhere (14). The full-length MnSOD cDNA fragment (18) was subcloned into the EcoRI site of the pcDNA3 plasmid, resulting in MnSOD sense and antisense expression constructs. The vector inserts were verified by sequence analysis.…”

Comparison of the crystal structures of genetically engineered human manganese superoxide dismutase and manganese superoxide dismutase from thermus thermophilus: Differences in dimer–dimer interaction

“…Human MnSOD is an enzyme with a subunit molecular weight of 22,200 daltons, consisting of 198 amino acids of known sequence (Barra et al, 1984;Beck et al, 1987).…”