Human Monoclonal Antibodies Neutralizing Human Cytomegalovirus

Masuho, Yasuhiko; Matsumoto, Yoh-ichi; Sugano, Toru; Fujinaga, Shigeki; Minamishima, Yoichi

doi:10.1099/0022-1317-68-5-1457

Cited by 60 publications

(32 citation statements)

References 16 publications

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“…In contrast, hyperimmune immunoglobulin was able to neutralize infectivity after virus adsorption and, whereas neutralization was enhanced in the presence of complement, the virus was neutralized at low antibody concentrations even in the absence of complement. These results are consistent with previous reports of the requirement of complement for neutralization of MCMV and HCMV by some sera (Kim & Carp, 1973;Rasmussen et al, 1985;Masuho et al, 1987;, but not by others (Rasmussen et al, 1984).…”

Section: Discussionsupporting

confidence: 93%

Characterization of neutralizing monoclonal antibodies to murine cytomegalovirus

Farrell

Shellam

1990

Journal of General Virology

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Monoclonal antibodies (MAbs) and polyclonal antibodies raised in mice to murine cytomegalovirus (MCMV) were characterized in vitro by their virusneutralizing activity and their reactivity with MCMV polypeptides and MCMV-infected mouse embryo fibroblasts (MEF). MCMV was neutralized by the MAbs prior to virus adsorption to MEF by a complement-dependent mechanism. Although the neutralization of MCMV prior to virus adsorption to MEF by polyclonal antibodies was enhanced in the presence of complement, MCMV was also neutralized by a complement-independent mechanism after virus adsorption. No correlation was observed between the level of neutralization of MCMV and the ability of MAbs or polyclonal antibodies to interfere with the binding of the virus to MEF. As the neutralization of MCMV with polyclonal antibodies by both complement-dependent and -independent mechanisms may reflect the interaction of antibodies with different specificities, the ability of each MAb to interact with a second MAb was investigated. One MAb, 1E8, inhibited the neutralizing activity of several other MAbs. Several MAbs reacted with multiple polypeptides by immunoprecipitation and Western blotting analysis; MAb AC1 cross-reacted with a neutralizing 92K/98K MCMV domain and a 70K ribonucleoprotein, the latter with which sera from patients with connective tissue diseases also reacted. This suggests that an homology exists between these proteins, which may lead to the development ofautoimmune manifestations in vivo.

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Section: Discussionsupporting

confidence: 93%

Characterization of neutralizing monoclonal antibodies to murine cytomegalovirus

Farrell

Shellam

1990

Journal of General Virology

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“…The requirement for complement in the neutralization assay for MCMV confirms the results of Kim & Carp (1973), and supports observations with other viruses, including human cytomegalovirus, herpes simplex virus type 1, simian cytomegalovirus and rubella virus which are also neutralized more efficiently by antibody after the addition of complement (Masuho et al, 1987;Yoshino & Taniguchi, 1965). Although the role of complement is generally attributed to an increase in the bulk of protein on the virion surface which hinders absorption, it may enhance virus neutralization by directly lysing the virus-antibody mixture as has been shown for many enveloped viruses including avian infectious bronchitis virus (Berry & Almeida, 1968), mouse leukaemia virus (Oroszlan & Gilden, 1970), Moloney leukaemia virus (Oldstone, 1975) and rubella virus (Schluederberg et al, 1976).…”

Section: Discussionsupporting

confidence: 82%

Antibody Responses to Murine Cytomegalovirus in Genetically Resistant and Susceptible Strains of Mice

Lawson

Grundy

Shellam

1988

Journal of General Virology

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“…Moreover, human anti-gB monoclonal antibodies (MAbs) neutralize HCMV in vitro (Masuho et al, 1987). There is also evidence for activation of helper T cells and cytotoxic T lymphocytes (CTLs) by gB in naturally seropositive humans (Borysiewicz et al, 1988;Liu et al, 199I ;Riddell et aI., 1991).…”

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confidence: 99%

Murine cytotoxic T cell response specific for human cytomegalovirus glycoprotein B (gB) induced by adenovirus and vaccinia virus recombinants expressing gB

Berencsi

Rando

deTaisne

et al. 1993

Journal of General Virology

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A murine model of the cytotoxic T lymphocyte (CTL) response to glycoprotein B (gB) of human cytomegalovirus (HCMV) was developed based on the use of adenovirus (Ad) and vaccinia virus (Vac) recombinants expressing gB. Mice of different major histocompatibility haplotypes [CBA (H-2k), BALB/k (H-2 k) and BALB/c (H-2d)] infected with the Ad-gB recombinant developed an Ad-specific CTL response. However, only the H-2 k mice developed a significant HCMV gBspecific CTL response, as indicated by the major histocompatibility complex class I-restricted lysis of Vac strain Copenhagen (VacC)-gB recombinant-infected target cells by H-2 k mouse immune spleen cells. The VacC-gB recombinant elicited only a weak gB-specific CTL response in these mice, indicating that the observed gB-specific CTL response in mice is dependent on the expression vector used for immunization. The gBspecific cytotoxicity observed in H-2 k mice was mediated by the CD8 lymphocyte subset.

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Human Monoclonal Antibodies Neutralizing Human Cytomegalovirus

Cited by 60 publications

References 16 publications

Characterization of neutralizing monoclonal antibodies to murine cytomegalovirus

Characterization of neutralizing monoclonal antibodies to murine cytomegalovirus

Antibody Responses to Murine Cytomegalovirus in Genetically Resistant and Susceptible Strains of Mice

Murine cytotoxic T cell response specific for human cytomegalovirus glycoprotein B (gB) induced by adenovirus and vaccinia virus recombinants expressing gB

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