Human multipotent mesenchymal stromal cell transcriptome changes in response to PDGF-BB 

Abstract

Activation of multipotent mesenchymal stromal cells (MSCs) is a central part of tissue response to the damage. Platelet-derived growth factor (PDGF-BB), which is abundantly released in the damaged area, potently stimulates the proliferation and migration of MSCs. Recent evidences indicate that tissue injury associates with the accumulation of senescent cells, including ones of MSC origin. Therefore, we hypothesized that PDGF-BB induces MSC senescence. To evaluate mechanisms of early activation of MSCs by PDGF-BB, we performed transcriptome profiling of human MSCs isolated from adipose tissue after exposure to PDGF-BB for 12 and 24 hours. We demonstrated that PDGF-BB induced the expression of senescence markers in MSCs. However, further experimental validation of transcriptomic data clearly indicated that PDGF-BB exerts mitogenic and pro-migratory effects on MSCs, but did not significantly stimulate their senescence.