Human natural cytotoxic lymphocytes: Definition by a monoclonal antibody of a subset which kills an anchorage-dependent target cell line but not the K-562 cell line

Rola‐Pleszczynski, Marek; Lieu, Hien

doi:10.1016/0008-8749(83)90166-1

Cited by 10 publications

(3 citation statements)

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“…They express the HNC-1A3 antigen but not (<2%) the HNK-1 (Leu-7) antigen characteristic of human NK cells (14). In further contrast to NK cells, HNC cells do not express the T10 (OKT10) antigen.…”

Section: Resultsmentioning

confidence: 93%

“…Since our original description (14) of a monoclonal antibody, HNC-1A3, which defines human natural cytotoxic effector cells, these lymphocytes have been further studied using other monoclonal antibodies (Table I). HNC-1A3+ cells were found to be negative for OKT10, Leu-7, and anti-HLA-DR, while '60% of them were labeled with Leu-11, a monoclonal antibody recognizing the Fc receptor.…”

Section: Resultsmentioning

confidence: 99%

“…We have recently produced a mouse monoclonal antibody (HNC-1A3) which defines a subpopulation of human natural cytotoxic cells (14). In this report, we present new evidence that spontaneous cytotoxic activity in man is also mediated by at least two distinct lymphocyte subsets distinguishable from each other by target cell specificity, cell surface markers, and sensitivity to biological response modifiers.…”

Section: Introductionmentioning

confidence: 90%

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Membrane markers, target cell specificity, and sensitivity to biological response modifiers distinguish human natural cytotoxic from human natural killer cells.

Rola‐Pleszczynski¹,

Lieu²,

Sullivan³

et al. 1985

J. Clin. Invest.

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In the present report, we provide evidence for the distinct existence of a human natural cytotoxic (HNC) cell. This HNC cell can be identified by the monoclonal antibody HNC-1A3 and by the absence of the T10 antigen, other antigenic markers being shared, at least in part, with natural killer (NK) cells, T cells, or monocytes. In addition, the HNC cell preferentially kills the MA-160 target, the herpes simplex virus-i-infected MA-160 cell line, and the two human tumor cell lines HEp-2 and HF-2. It has weak lytic activity against the NK-sensitive K562 cell line or its relatively NK-resistant clone I subline. The cytotoxic activity of the HNC cell is not augmented by interferon but is markedly enhanced by interleukin 2 and by a measles-virusinduced factor (MVF). Furthermore, it is not inhibited by cyclosporin A (CsA), in contrast to NK cell cytotoxicity against the K562 target cell line which is augmented by interferon, inhibited by CsA, and not affected by MVF. These data suggest that spontaneously cytotoxic cells may belong to more than one subset of human lymphocytes, and that HNC cells may be defined in man using membrane markers, target cell specificity, and sensitivity to biological response modifiers.

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Section: Resultsmentioning

confidence: 93%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 90%

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Membrane markers, target cell specificity, and sensitivity to biological response modifiers distinguish human natural cytotoxic from human natural killer cells.

Rola‐Pleszczynski¹,

Lieu²,

Sullivan³

et al. 1985

J. Clin. Invest.

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In vitro cellular cytotoxicity for a human colon cancer cell line by mucosal mononuclear cells of patients with colon cancer and other disorders

Beeken

Gundel

Andre-Ukena

et al. 1985

Cancer

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In vitro cellular cytotoxicity of mononuclear cells of intestinal mucosa and peripheral blood for a colon cancer cell target was measured in patients with colon cancer and other disorders requiring resection. Four‐ and 24‐hour cytotoxicity assays were conducted using selenium 75 (75Se)‐labeled RPMI‐4788 human colon cancer target cells grown in culture. In the cancer group mean cytotoxicity was 30.4% at 24 hours for peripheral blood effectors and 8.0% for effectors from normal mucosa. Values in patients with Crohn's disease were 10.4% for blood and 17.2% for effectors from normal mucosa, and 13.6% and 18.5%, respectively, for blood and abnormal mucosa. Values in patients with other diseases were 25% for blood and 14.7% for mucosa. Mean cytotoxicity at 4 hours did not exceed 6.4% for any group, and assays in autologous serum gave results similar to tests in calf serum. In additional studies, K 562 chronic leukemia cells were somewhat more sensitive to lysis than RPMI‐4788 by blood mononuclear cells, but there was no lysis of K 562 by mucosal populations that were cytotoxic for RPMI‐4788. There was no competitive inhibition by either target cell for the other. It was concluded that 75Se RPMI‐4788 colon cancer cells are suitable targets for evaluating in vitro cytotoxicity by intestinal mucosal cells and that mucosal cytotoxicity in patients with colon cancer is depressed compared to cytotoxicity by peripheral blood effectors.

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Immunology of the liver

Jakó

1991

Gammopathy

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Human natural cytotoxic lymphocytes: Definition by a monoclonal antibody of a subset which kills an anchorage-dependent target cell line but not the K-562 cell line

Cited by 10 publications

References 10 publications

Membrane markers, target cell specificity, and sensitivity to biological response modifiers distinguish human natural cytotoxic from human natural killer cells.

Membrane markers, target cell specificity, and sensitivity to biological response modifiers distinguish human natural cytotoxic from human natural killer cells.

In vitro cellular cytotoxicity for a human colon cancer cell line by mucosal mononuclear cells of patients with colon cancer and other disorders

Immunology of the liver

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