Human olfactory mesenchymal stromal cells co-expressing horizontal basal and ensheathing cell proteins in culture

Ayala-Grosso, Carlos; Pieruzzini, Rosalinda; Vargas-Saturno, Leslie; Cardier, José E.

doi:10.7705/biomedica.4762

Cited by 6 publications

(4 citation statements)

References 40 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Assessment of the four markers by immunofluorescence microscopy (IMF, Figure D,E) was confirmatory of those obtained by flow cytometry. Hence, MSCs/MenSCs are naturally neurogenic. − Since menstrual stromal cells (MenSCs) are well characterized and present minimal technical limitations, and minor ethical issues, this biological source cultured for 7 days in NeuroForsk medium has become one of the easiest methods to obtain a consistent, ready-to-use source of functional neurogenic cells to further investigate environmental and/or genetic aspects of PD. Therefore, we have used MenSC-derived dopaminergic-like neurons (DALNs) to inquire into the effect of CBD once exposed to the ambient pesticides PQ 2+ /MB, used as model neurotoxins in PD.…”

Section: Resultsmentioning

confidence: 99%

Cannabidiol Protects Dopaminergic-like Neurons against Paraquat- and Maneb-Induced Cell Death through Safeguarding DJ-1CYS¹⁰⁶ and Caspase 3 Independently of Cannabinoid Receptors: Relevance in Parkinson’s Disease

Mendivil-Perez,

Felizardo-Otalvaro,

Jimenez-Del-Rio

et al. 2023

ACS Chem. Neurosci.

View full text Add to dashboard Cite

Parkinson's disease (PD), a progressive neurodegenerative movement disorder, has reached pandemic status worldwide. This neurologic disorder is caused primarily by the specific deterioration of dopaminergic (DAergic) neurons in the substantia nigra pars compacta (SNc). Unfortunately, there are no therapeutic agents that slow or delay the disease progression. Herein, menstrual stromal cell-derived dopamine-like neurons (DALNs) intoxicated with paraquat (PQ 2+ )/maneb (MB) were used as a model system to elucidate the mechanism by which CBD protects the neural cell from apoptosis in vitro. According to immunofluorescence microscopy, flow cytometry, cell-free assay, and molecular docking analysis, we demonstrate that CBD offers protection to DALNs against PQ 2+ (1 mM)/ MB (50 μM)-induced oxidative stress (OS) by simultaneously (i) decreasing reactive oxygen species (ROS: O 2•− , H 2 O 2 ), (ii) maintaining the mitochondrial membrane potential (ΔΨ m ), (iii) directly binding to stress sensor protein DJ-1, thereby blunting its oxidation from DJ-1CYS 106 -SH into DJ-1CYS 106 -SO 3 , and (iv) directly binding to pro-apoptotic protease protein caspase 3 (CASP3), thereby disengaging neuronal dismantling. Furthermore, the protective effect of CBD on DJ-1 and CASP3 was independent of CB1 and CB2 receptor signaling. CBD also re-established the Ca 2+ influx in DALNs as a response to dopamine (DA) stimuli under PQ 2+ /MB exposure. Because of its powerful antioxidant and antiapoptotic effects, CBD offers potential therapeutic utility in the treatment of PD.

show abstract

Section: Resultsmentioning

confidence: 99%

Cannabidiol Protects Dopaminergic-like Neurons against Paraquat- and Maneb-Induced Cell Death through Safeguarding DJ-1CYS¹⁰⁶ and Caspase 3 Independently of Cannabinoid Receptors: Relevance in Parkinson’s Disease

Mendivil-Perez,

Felizardo-Otalvaro,

Jimenez-Del-Rio

et al. 2023

ACS Chem. Neurosci.

View full text Add to dashboard Cite

show abstract

“…Harvesting techniques for OE in humans [ 28 , 29 , 30 , 43 , 44 ] and rats [ 45 ] are widely available; however they are scarce for mice [ 36 ]. This called for the development of our own harvesting surgical technique.…”

Section: Methodsmentioning

confidence: 99%

“…First, many studies about in vitro regrowth of olfactory axons use fetal bovine serum [ 28 ], containing a mixture of poorly characterized growth factors or Matrigel [ 29 ], a mixture of extracellular matrix proteins (ECM) [ 30 ]. Therefore, information on the effect of a single growth factor on axonal regrowth are scarce.…”

Section: Introductionmentioning

confidence: 99%

Axonal Regrowth of Olfactory Sensory Neurons In Vitro

Sipione

Liaudet

Rousset

et al. 2023

IJMS

View full text Add to dashboard Cite

One of the most prevalent causes of olfactory loss includes traumatic brain injury with subsequent shearing of olfactory axons at the level of the cribriform plate (anterior skull base). Scar tissue at this level may prevent axonal regrowth toward the olfactory bulb. Currently, there is no cure for this debilitating and often permanent condition. One promising therapeutic concept is to implant a synthetic scaffold with growth factors through the cribriform plate/scar tissue to induce neuroregeneration. The first step toward this goal is to investigate the optimum conditions (growth factors, extracellular matrix proteins) to boost this regeneration. However, the lack of a specifically tailored in vitro model and an automated procedure for quantifying axonal length limits our ability to address this issue. The aim of this study is to create an automated quantification tool to measure axonal length and to determine the ideal growth factors and extracellular proteins to enhance axonal regrowth of olfactory sensory neurons in a mouse organotypic 2D model. We harvested olfactory epithelium (OE) of C57BL/6 mice and cultured them during 15 days on coverslips coated with various extracellular matrix proteins (Fibronectin, Collagen IV, Laminin, none) and different growth factors: fibroblast growth factor 2 (FGF2), brain-derived neurotrophic factor (BDNF), glial cell-derived neurotrophic factor (GDNF), nerve growth factor (NGF), retinoic acid (RA), transforming growth factor β (TGFβ), and none. We measured the attachment rate on coverslips, the presence of cellular and axonal outgrowth, and finally, the total axonal length with a newly developed automated high-throughput quantification tool. Whereas the coatings did not influence attachment and neuronal outgrowth rates, the total axonal length was enhanced on fibronectin and collagen IV (p = 0.001). The optimum growth factor supplementation media to culture OE compared to the control condition were as follows: FGF2 alone and FGF2 from day 0 to 7 followed by FGF2 in combination with NGF from day 7 to 15 (p < 0.0001). The automated quantification tool to measure axonal length outperformed the standard Neuron J application by reducing the average analysis time from 22 to 3 min per specimen. In conclusion, robust regeneration of murine olfactory neurons in vitro can be induced, controlled, and efficiently measured using an automated quantification tool. These results will help advance the therapeutic concept closer toward preclinical studies.

show abstract

“…To induce neural differentiation, hO-MSCs were seeded in SFM supplemented with N2, RA and forskolin. In this regard, it was shown that SFM led to the differentiation of hO-MSCs into neurons and astroglial cells [ 96 ].…”

Section: Alternatives To Animal Serum In Msc Culturesmentioning

confidence: 99%

Serum-Free Cultures: Could They Be a Future Direction to Improve Neuronal Differentiation of Mesenchymal Stromal Cells?

Schepici

Mazzon

2022

IJMS

View full text Add to dashboard Cite

Mesenchymal stem/stromal cells (MSCs) are undifferentiated cells with multilinear potential, known for their immunomodulatory and regenerative properties. Although the scientific community is working to improve their application, concerns limit their use to repair tissues following neurological damage. One of these obstacles is represented by the use of culture media supplemented with fetal bovine serum (FBS), which, due to its xenogenic nature and the risk of contamination, has increased scientific, ethical and safety problems. Therefore, the use of serum-free media could improve MSC culture methods, avoiding infectious and immunogenic transmission problems as well as MSC bioprocesses, without the use of animal components. The purpose of our review is to provide an overview of experimental studies that demonstrate that serum-free cultures, along with the supplementation of growth factors or chemicals, can lead to a more defined and controlled environment, enhancing the proliferation and neuronal differentiation of MSCs.

show abstract

Human olfactory mesenchymal stromal cells co-expressing horizontal basal and ensheathing cell proteins in culture

Cited by 6 publications

References 40 publications

Cannabidiol Protects Dopaminergic-like Neurons against Paraquat- and Maneb-Induced Cell Death through Safeguarding DJ-1CYS¹⁰⁶ and Caspase 3 Independently of Cannabinoid Receptors: Relevance in Parkinson’s Disease

Cannabidiol Protects Dopaminergic-like Neurons against Paraquat- and Maneb-Induced Cell Death through Safeguarding DJ-1CYS¹⁰⁶ and Caspase 3 Independently of Cannabinoid Receptors: Relevance in Parkinson’s Disease

Axonal Regrowth of Olfactory Sensory Neurons In Vitro

Serum-Free Cultures: Could They Be a Future Direction to Improve Neuronal Differentiation of Mesenchymal Stromal Cells?

Contact Info

Product

Resources

About

Human olfactory mesenchymal stromal cells co-expressing horizontal basal and ensheathing cell proteins in culture

Cited by 6 publications

References 40 publications

Cannabidiol Protects Dopaminergic-like Neurons against Paraquat- and Maneb-Induced Cell Death through Safeguarding DJ-1CYS106 and Caspase 3 Independently of Cannabinoid Receptors: Relevance in Parkinson’s Disease

Cannabidiol Protects Dopaminergic-like Neurons against Paraquat- and Maneb-Induced Cell Death through Safeguarding DJ-1CYS106 and Caspase 3 Independently of Cannabinoid Receptors: Relevance in Parkinson’s Disease

Axonal Regrowth of Olfactory Sensory Neurons In Vitro

Serum-Free Cultures: Could They Be a Future Direction to Improve Neuronal Differentiation of Mesenchymal Stromal Cells?

Contact Info

Product

Resources

About

Cannabidiol Protects Dopaminergic-like Neurons against Paraquat- and Maneb-Induced Cell Death through Safeguarding DJ-1CYS¹⁰⁶ and Caspase 3 Independently of Cannabinoid Receptors: Relevance in Parkinson’s Disease

Cannabidiol Protects Dopaminergic-like Neurons against Paraquat- and Maneb-Induced Cell Death through Safeguarding DJ-1CYS¹⁰⁶ and Caspase 3 Independently of Cannabinoid Receptors: Relevance in Parkinson’s Disease