Human papillomavirus genotyping and integration in ovarian cancer Saudi patients

Abstract

BackgroundHuman papillomavirus (HPV) is associated with different malignancies but its role in the pathogenesis of ovarian cancer is controversial. This study investigated the prevalence, genotyping and physical state of HPV in ovarian cancer Saudi patients.MethodsHundred formalin fixed paraffin embedded (FFPE) ovarian carcinoma tissues and their normal adjacent tissues (NAT) were included in the study. HPV was detected by nested polymerase chain reaction (PCR) using degenerated HPVL1 consensus primer pairs MY09/MY11 and GP5+/GP6 + to amplify a broad spectrum of HPV genotypes in a single reaction. The HPV positive samples were further genotyped using DNA sequencing. The physical state of the virus was identified using Amplification of Papillomavirus Oncogene Transcripts (APOT) assay in the samples positive for HPV16 and/or HPV18.ResultsHigh percentage of HPV (42%) was observed in ovarian carcinoma compared to 8% in the NAT. The high-risk HPV types 16, 18 and 45 were highly associated with the advanced stages of tumor, while low-risk types 6 and 11 were present in NAT. In malignant tissues, HPV-16 was the most predominant genotype followed by HPV-18 and -45. The percentage of viral integration into the host genome was significantly high (61.1%) compared to 38.9% episomal in HPV positive tumors tissues. In HPV18 genotype the percentage of viral integration was 54.5% compared to 45.5% episomal.ConclusionThe high risk HPV genotypes in ovarian cancer may indicate its role in ovarian carcinogenesis. The HPV vaccination is highly recommended to reduce this type of cancer.

Effect of FHIT loss and p53 mutation on HPV-infected lung carcinoma development

“…HPV infection, particularly high-risk HPV16/18 infection, has been identified as a risk factor for the development of cervical cancer, lung cancer and other malignant tumors (1,2,17,18). Previous studies have reported that the involvement of HPV16/18 infection in lung tumorigenesis may be mediated through FHIT and p53 (7,13,14,19,20).…”

“…Thus, HPVL1 detection can indicate HPV infection. High-risk HPV16 and HPV18 are the most common oncogenic types, leading to the development of cervical carcinomas and a variety of different types of reproductive system malignant tumor (1)(2)(3)(4). Lung cancer is one of the most common cancers and the leading cause of cancer-associated mortality in the world.…”

Methylation of SFRPs and APC Genes in Ovarian Cancer Infected with High Risk Human Papillomavirus

“…To identify the genotypes of HPV, all positive PCR products were analyzed by direct DNA sequencing according to our recent study (Al-Shabanah et al, 2013). Chromatograms with sharp peaks and quality values ≥20 DOI:http://dx.doi.org/10.7314/APJCP.2014.15.6…”

Prevalence, spectrum, and founder effect of BRCA1 and BRCA2 mutations in epithelial ovarian cancer from the Middle East

“…OC in Saudi Arabia accounts for 2.9% of all cancers (Al‐Shahrani et al, ), whereas it accounts for 3.4% in Western population (Bray et al, ). Furthermore, OC in Saudi population has unique characteristics such as lower median age at presentation (55 years in Saudi vs. 65 years in Western population; Alghamdi et al, ; Al‐Shabanah et al, ; Torre et al, ), advanced stage (Al‐Badawi et al, ), and higher incidence of mucinous carcinomas (19% vs. 3% in Western population; Al‐Badawi et al, ; Perren, ; Xu, Rush, Rickett, & Coward, ). It is believed that nearly 5–12% of invasive EOC are caused by hereditary susceptibility (Risch et al, ; Whittemore, ).…”