Human papillomavirus infection in nasal polyps in a Chinese population

Pei, Fei; Chen, Xiaoping; Zhang, Yi; Wang, Yan; Chen, Qun; Tan, Xue-jun; Zhang, Jingfei; Xue, Xiaocheng; Wu, Jian; Qing, Zhi-Rong

doi:10.1099/vir.0.031955-0

Cited by 22 publications

(32 citation statements)

References 19 publications

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“…On the contrary, HPV infection was absent in SNP-WAD patients and in SNP-WoAD patients without a relapsing disease. These results confirm the importance of the methodological approach in the selection of patients for the study of this pathology and support previous results [33], where HPV-11 was observed as the prevalent infection SNP. Interestingly, HPV infection was restricted to epithelial cells, as it is known to be highly tropic for epithelial cells, while no infection sign was evidenced in fibroblast cells.…”

Section: Discussionsupporting

confidence: 92%

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Infection and HLA-G Molecules in Nasal Polyposis

Rizzo

Mozzillo²,

Bortolotti

et al. 2014

Journal of Immunology Research

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Sinonasal polyposis (SNP) is a chronic inflammatory pathology with an unclear aetiopathogenesis. Human papillomavirus (HPV) infection is one candidate for the development of SNP for its epithelial cell trophism, hyperproliferative effect, and the induction of immune-modulatory molecules as HLA-G. We enrolled 10 patients with SNP without concomitant allergic diseases (SNP-WoAD), 10 patients with SNP and suffering from allergic diseases (SNP-WAD), and 10 control subjects who underwent rhinoplasty. We analyzed the presence of high- and low-risk HPV DNA and the expression of membrane HLA-G (mHLA-G) and IL-10 receptor (IL-10R) and of soluble HLA-G (sHLA-G) and IL-10 by polyp epithelial cells. The results showed the presence of HPV-11 in 50% of SNP-WoAD patients (OR:5.5), all characterized by a relapsing disease. HPV-11 infection was absent in nonrelapsing SNP-WoAD patients, in SNP-WAD patients and in controls, supporting the hypothesis that HPV-11 increases risk of relapsing disease. HPV-11 positive SNP-WoAD patients presented with mHLA-G and IL-10R on epithelial cells from nasal polyps and showed secretion of sHLA-G and IL-10 in culture supernatants. No HLA-G expression was observed in HPV negative polyps. These data highlight new aspects of polyposis aetiopathogenesis and suggest HPV-11 and HLA-G/IL-10 presence as prognostic markers in the follow-up of SNP-WoAD.

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Section: Discussionsupporting

confidence: 92%

“…Previous studies in literature have analyzed the role of HPV infection in SNP, demonstrating different correlation ranges [5, 6, 33]. We hypothesize that these differences may be due to the absence of a preselection of patients according to the presence or absence of allergic diseases that could be a confounder for the results observed.…”

Section: Discussionmentioning

confidence: 86%

Infection and HLA-G Molecules in Nasal Polyposis

Rizzo

Mozzillo²,

Bortolotti

et al. 2014

Journal of Immunology Research

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“…With the developments in laboratory medicine and the sensitive detection technology available in clinical practice, multiple infections by different HPV genotypes, including HR‐HPV and LR‐HPV, can be easily and accurately identified, making large public health screening for HPV genotyping in a clinic or health center possible. With its very efficient technique for molecular hybridization, flow‐through hybridization has been recognized as an appropriate tool for HPV genotyping . In this study, a newly developed technique that combines flow‐through hybridization and gene chip was used to perform HPV genotyping in women.…”

Section: Discussionmentioning

confidence: 99%

Distribution characteristics of different human papillomavirus genotypes in women in Wuhan, China

Xiang

Guan

Liu

et al. 2018

Clinical Laboratory Analysis

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BackgroundHuman papillomaviruses (HPVs) are strongly associated with the development of cervical carcinoma, and the distribution of HPV genotypes varies regionally.MethodsTo investigate the distribution characteristics of different genotypes of HPV infection in women in Wuhan, China, a total of 13 775 patients were enrolled over 2 years.ResultsOf these, 2436 patients were infected with HPVs, and the total infection rate was 17.68%. The infection rate of high‐risk HPV (HR‐HPV) was significantly higher (13.96%) than that of single low‐risk HPV (LR‐HPV; 3.72%). Among the HR‐HPV infections, the most common genotype was HPV 52 with an infection rate of 4.23%, followed by HPVs 16, 58, 39, and 51. The most common LR‐HPV genotype was HPV 81, followed by HPVs 6, 11, and 44. Patients under the age of 25 years were found to have the highest HPV infection rate (P < .05). After the age range of 51‐55 years, a downward trend in total HPVs and HR‐HPVs was observed. The HPV infection rate for a single genotype was higher than that for multiple HPVs (P < .01), and the detection rates in summer and winter were significantly higher than those in spring and autumn.ConclusionsThe results demonstrate that the distribution characteristics of various HPV genotype infections are associated with region and age and may be related to season. These data could be the basis for further epidemiological analysis into the control and prevention of HPV infection in this region.

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“…Flow-through hybridization is the most efficient method for molecular hybridization [9–11]. A newly developed biotechnology named HybriMax, combining two advanced techniques, the flow-through hybridization and gene chip, is developeded to be used in clinical practice for the detection and genotyping of 21 different types of HPVs at one test.…”

Section: Discussionmentioning

confidence: 99%

“…However, its main undeniable limitation is that HC-II cannot distinguish between different HPV genotypes definitely [5, 8]. Recently, a new HPV-genotyping method combining two advanced techniques, the flow-through hybridization and gene chip (HybriMax) has been used to detect and genotype HPV, which can distinguish 21 different types of HPV DNA in a single test and diagnose multiple infections [9–11]. In this study, we have evaluated the efficacy of HybriMax on cervical HPV genotyping through comparison of the results with Hybrid Capture II (HC-II) and in situ hybridization (ISH).…”

Section: Introductionmentioning

confidence: 99%

Sensitive HPV Genotyping Based on the Flow-Through Hybridization and Gene Chip

Tao

Zheng

Wang

et al. 2012

Journal of Biomedicine and Biotechnology

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Persistent infection of high-risk human papillomavirus (HPV) has been recognized as the direct cause of cervical carcinoma. Therefore, detection and genotyping of HPV are important to cervical-cancer screening. In this study, we have evaluated the efficacy of flow-through hybridization and gene chip (HybriMax) on HPV genotyping through comparison of the results with Hybrid Capture II (HC-II) and in situ hybridization (ISH). 591 women were classified into 6 groups according to their histological diagnoses. The overall accordance rate on 13 types of HPV genotypes between HybriMax and HC-II were 92.5% and 100% in the cancer group. The overall accordance was excellent with the Kappa index (KI) of 0.814. The value of KI in each group was 0.750 (normal cytological diagnosis), 0.781 (chronic cervicitis), 0.80 (condyloma acuminatum), 0.755 (cervical intraepithelial neoplasia (CIN) I), 0.723 (CIN II), and 0.547 (CIN III) (0.75 > KI > 0.4, good; KI ≥ 0.75, excellent). The 10 most common HPV subtype detected by HybriMax were 16, 52/58, 18, 33, 31, 81, 53, 68, and 66 in patients, and 16, 68, 18, 52, 58, 11, 53, 31/39, and 33 in normal controls. In conclusion, HybriMax is an efficient method for HPV genotyping and more suitable for clinical use.

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Human papillomavirus infection in nasal polyps in a Chinese population

Cited by 22 publications

References 19 publications

Infection and HLA-G Molecules in Nasal Polyposis

Infection and HLA-G Molecules in Nasal Polyposis

Distribution characteristics of different human papillomavirus genotypes in women in Wuhan, China

Sensitive HPV Genotyping Based on the Flow-Through Hybridization and Gene Chip

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