Human papillomavirus type distribution and HPV16 intratype diversity in southern Brazil in women with and without cervical lesions

Abstract

BACKGROUND Increasing evidence suggests that human papillomavirus (HPV) intratype variants (specific lineages and sublineages) are associated with pathogenesis and progression from HPV infection to persistence and the development of cervical cancer.OBJECTIVES This study aimed to verify the prevalence of HPV infection and distribution of HPV types and HPV16 variants in southern Brazil in women with normal cytology or intraepithelial lesions.METHODS HPV typing was determined by L1 gene sequencing. To identify HPV16 variants, the LCR and E6 regions were sequenced, and characteristic single nucleotide variants were identified.FINDINGS A total of 445 samples were studied, with 355 from cervical scrapes and 90 from cervical biopsies. HPV was detected in 24% and 91% of these samples, respectively. The most prevalent HPV types observed were 16 (cervical, 24%; biopsies, 57%) and 58 (cervical, 12%; biopsies, 12%). Seventy-five percent of the HPV16-positive samples were classified into lineages, with 88% defined as lineage A, 10% as lineage D, and 2% as lineage B.MAIN CONCLUSIONS This study identified a high frequency of European and North American HPV16 lineages, consistent with the genetic background of the human population in southern Brazil.

Human Papillomavirus 16 Lineage D is Associated with High Risk of Cervical Cancer in the Brazilian Northeast Region

“…Another study conducted in a different region in Brazil also found a high prevalence of the lineage A of HPV16 in samples of high-and low-grade lesions, including cervical cancer. 29 In contrast, the D lineage of HPV16 showed a significant association with cervical cancer, compared with high-grade lesions, suggesting that the lineage D of HPV16 is involved in the progression of HPV16 infection to cervical cancer. Although this is a cross-sectional study, the present study provides novel data on the association between specific lineages of HPV16 and cervical cancer.…”

“…Another study conducted in a different region in Brazil also found a high prevalence of the lineage A of HPV16 in samples of high- and low-grade lesions, including cervical cancer. 29…”

Human Papillomavirus 16 lineage D is associated with high risk of cervical cancer in the Brazilian Northeast region

“…In this study, the A lineage of HPV 16 was the most frequent and although there was a trend of association with high-grade lesions (CIN2/CIN3), it was not possible to demonstrate a difference between these high-grade lesions and cervical cancer. A study carried out in another region of Brazil also found a high prevalence of the A lineage of HPV16 in samples of high-and low-grade lesions, including cancer [30].…”

Virus Papiloma Humano. Un enemigo silencioso en el Ecuador y el mundo

“…Cada una de las teorías propuestas para esclarecer el efecto biológico de las coinfecciones por VPH en las células cervicales, se sustenta en evidencias de estudios poblacionales. Sin embargo, no existen investigaciones básicas que demuestren, desde el punto vista molecular y celular, las hipótesis propuestas por un sinnúmero de investigadores (196,197,200,201). Obviamente, esto se debe a las limitaciones para realizar estudios in vitro, por la incapacidad del VPH para replicarse en cultivos celulares, aunque se están desarrollando investigaciones para lograr este objetivo (202).…”