Human Parainfluenza Virus Type 3 (PIV3) Expressing the Hemagglutinin Protein of Measles Virus Provides a Potential Method for Immunization against Measles Virus and PIV3 in Early Infancy

Durbin, Anna P.; Skiadopoulos, Mario H.; McAuliffe, Josephine M.; Riggs, Jeffrey M.; Surman, Sonja R.; Collins, Peter L.; Murphy, Brian R.

doi:10.1128/jvi.74.15.6821-6831.2000

Cited by 52 publications

(44 citation statements)

References 63 publications

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“…The efficient production of infectious virus may be more sensitive to the foreign gene insertion site and altered viral gene expression in the context of LLC-MK2 cells than respiratory tract tissues. Our findings on the rSeV vector are similar to those showing that rHPIV3-MeHA(P-M), a recombinant HPIV3 virus with the measles virus HA gene inserted into the P-M gene junction, has delayed growth in LLC-MK2 cells compared to rHPIV3-MeHA(HN-L) and yet grows to similar levels in the upper and lower respiratory tracts of hamsters (34). However, this does not suggest that any paramyxovirus vector attenuated in vitro by foreign gene insertion will remain fully fit for growth in vivo.…”

Section: Discussionsupporting

confidence: 73%

Influence of Antigen Insertion Site and Vector Dose on Immunogenicity and Protective Capacity in Sendai Virus-Based Human Parainfluenza Virus Type 3 Vaccines

Mason

Elbahesh

Russell

2013

J Virol

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Section: Discussionsupporting

confidence: 73%

Influence of Antigen Insertion Site and Vector Dose on Immunogenicity and Protective Capacity in Sendai Virus-Based Human Parainfluenza Virus Type 3 Vaccines

Mason

Elbahesh

Russell

2013

J Virol

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“…Expression of foreign proteins by several members of the Paramyxoviridae has been reported (Bukreyev et al, 1996;Mebatsion et al, 1996;He et al, 1997;Johnson et al, 1997;Roberts et al, 1998;Baron et al, 1999;Singh & Billeter, 1999;Bailly et al, 2000;Buchholz et al, 2000;Durbin et al, 2000;Krishnamurthy et al, 2000;Huang et al, 2001;Nakaya et al, 2001). In most of these cases, the foreign gene was inserted at a specific position in the viral genome and no systematic attempts were made to examine the effects of the position of the foreign gene on expression levels and virus replication.…”

Section: Discussionmentioning

confidence: 99%

Recombinant Newcastle disease virus as a viral vector: effect of genomic location of foreign gene on gene expression and virus replication

Zhao¹,

Peeters²

2003

Journal of General Virology

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Newcastle disease virus (NDV) was examined for its suitability as a vector for the expression and delivery of foreign genes for vaccination and gene therapy. A reporter gene encoding human secreted alkaline phosphatase (SEAP) was inserted as an additional transcription unit at four different positions in the NDV genome, between the NP and P, M and F, and HN and L genes and behind the L gene. Eight infectious recombinant NDV (rNDV) viruses, four in the non-virulent strain NDFL and four in the virulent derivative NDFLtag, were generated by reverse genetics. SEAP expression levels, replication kinetics and virus yield were examined. Replication kinetics of the rNDV viruses in primary chicken embryo fibroblasts showed that the insertion of an additional gene resulted in a delay in the onset of replication. This effect was most prominent when the gene was inserted between the NP and P genes. With the exception of the strain that carried the SEAP gene behind the L gene, all recombinant strains expressed high levels of SEAP, both in cell culture and in embryonated chicken eggs. In embryonated eggs, the rNDV viruses showed a 2?6-to 5?6-fold (NDFL) or 2?1-to 8?1-fold (NDFLtag) reduction in yield compared with the parent strains. These results show that foreign genes can be inserted at different positions in the NDV genome without severely affecting replication efficiency or virus yield.

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“…A plethora of live-attenuated viruses and chimeric viruses have been generated using reverse genetics (11,12,22,32,33,35), and the same can now be envisaged for hMPV. For instance, recombinant hMPV strains harboring the surface glycoproteins of both serotype A and B isolates of hMPV can be generated that may induce a broad antibody response in infected hosts.…”

Section: Discussionmentioning

confidence: 99%

Recovery of Human Metapneumovirus Genetic Lineages A and B from Cloned cDNA

Herfst

Graaf

Schickli³

et al. 2004

J Virol

112

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Human metapneumovirus (hMPV) is a newly discovered pathogen associated with respiratory tract illness, primarily in young children, immunocompromised individuals, and the elderly. The genomic sequence of the prototype hMPV isolate NL/1/00 without the terminal leader and trailer sequences has been reported previously. Here we describe the leader and trailer sequences of two hMPV isolates, NL/1/00 and NL/1/99, representing the two main genetic lineages of hMPV. Minigenome constructs in which the green fluorescent protein or chloramphenicol acetyltransferase genes are flanked by the viral genomic ends derived from both hMPV lineages and transcribed using a T7 RNA polymerase promoter-terminator cassette were generated. Cotransfection of minigenome constructs with plasmids expressing the polymerase complex components L, P, N, and M2.1 in 293T or baby hamster kidney cells resulted in expression of the reporter genes. When the minigenome was replaced by a sense or antisense full-length cDNA copy of the NL/1/00 or NL/1/99 viral genomes, recombinant virus was recovered from transfected cells. Viral titers up to 10 7.2 and 10 5.7 50% tissue culture infective dose/ml were achieved with the sense and antisense plasmids, respectively. The recombinant viruses replicated with kinetics similar to those of the parental viruses in Vero cells. This reverse genetics system provides an important new tool for applied and fundamental research.

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Human Parainfluenza Virus Type 3 (PIV3) Expressing the Hemagglutinin Protein of Measles Virus Provides a Potential Method for Immunization against Measles Virus and PIV3 in Early Infancy

Cited by 52 publications

References 63 publications

Influence of Antigen Insertion Site and Vector Dose on Immunogenicity and Protective Capacity in Sendai Virus-Based Human Parainfluenza Virus Type 3 Vaccines

Influence of Antigen Insertion Site and Vector Dose on Immunogenicity and Protective Capacity in Sendai Virus-Based Human Parainfluenza Virus Type 3 Vaccines

Recombinant Newcastle disease virus as a viral vector: effect of genomic location of foreign gene on gene expression and virus replication

Recovery of Human Metapneumovirus Genetic Lineages A and B from Cloned cDNA

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