Human Placenta Feeder Layers Support Undifferentiated Growth of Primate Embryonic Stem Cells

Abstract

Various undifferentiated embryonic stem (ES) cells can grow on mouse embryonic fibroblast (MEF) feeders. However, the risk of zoonosis from animal feeders to human ES cells generally excludes the clinical use of these human ES cells. We have found that human placenta is a useful source of feeder cells for the undifferentiated growth of primate ES cells. As on MEF feeders, primate ES cells cultured on human amniotic epithelial (HAE) feeder cells and human chorionic plate (HCP) cells had undifferentiated growth. The cultured primate ES cells expressed Oct‐4, alkaline phosphatase, and SSEA‐4. The primate ES cells on HAE feeder cells produced typical immature teratomas in vivo after injection into severe combined immunodeficient mice. Human placenta is quite novel and important because it would provide a relatively available source of feeders for the growth of human ES cells for therapeutic purposes that are also free of ethical complications.

Amniotic stem cells repair ureteric defect: a study to evaluate the feasibility of amniotic membrane as a graft in surgical reconstruction

“…Moreover, it has been observed that limbal epithelial cells grown on amniotic membrane have a much faster and a more mature basement membrane formation than normal wound healing models. It has been suggested that the amniotic membrane can serve as a feeder layer [10] and could have promoted basement membrane formation. It is expected that amnion may have acted in a similar way in our study by providing a suitable environment for basement membrane formation and aiding earlier epithelialization.…”

“…This was followed by the use of amniotic membrane in the treatment of skin wounds, burn injuries, chronic leg ulcers, prevention of tissue adhesion in surgical procedures and also for ocular surface reconstruction [3][4][5][6][7][8][9]. Moreover, it has been reported that the human placental tissue acts as a source of feeder cells for the growth of primate embryonic stem cells [10].…”

Preparation of Amniotic Membrane and It’s Application in the Treatment of Skin Loss and Lyell's Syndrome (Toxic Epidermal Necrolysis): Current State and New Opportunities

“…Moreover, it was found that the amniotic membrane is a biodegradable scaffold with unique biochemical and mechanical properties conducive to nerve regeneration [30]. It has also been presented that decellurized amnion may be used as a nourishing layer for a part of stem cells necessary for neuronal differentiation [31]. It can also serve as a scaffold for chondrocytes and be used for cartilage regeneration [32].…”

Directed Differentation of Human Embryonic Stem Cells in Combination of Biomaterials

“…Otherwise, hESC in prolonged culture in vitro may cause higher karyotype instability and uncontrollable spontaneous differentiation phenomenon, which also confine manipulation in clinical application [3] . Accordingly, many research groups attempt to use various kinds of human-source cells as feeders, such as Methodological Advances in the Culture, Manipulation and Utilization of Embryonic Stem Cells for Basic and Practical Applications 160 human embryo fibroblast [4] , adult fetal muscle fibroblast, adult skin and adult fallopian tube, human marrow stromal cells (hMSC) [5] , human foreskin fibroblast (hFF) [6] , human placenta-derived feeder (HPC) [7] , human amniotic epithelial (HAE) [8] , and post natal human fibroblast. The number of colonies, number of cells produced, and cell survival rates, all of these indexes are significantly higher on MEF than on human feeder cells (P < 0.01) and the number of AP-positive colonies and cell quantity are also significantly higher on hMSC than on hFF (P < 0.01).…”