Human plasma as a dermal scaffold for the generation of a completely autologous bioengineered skin

Abstract

Our data demonstrate that this new dermal equivalent allows for (1) generation of large bioengineered skin surfaces, (2) restoration of both the epidermal and dermal skin compartments, and (3) functional epidermal stem-cell preservation.

Current approaches and perspectives in human keratinocyte-based gene therapies

“…13 In our setting, correction was achieved by using a conventional retroviral vector and a cultured skin equivalent to accomplish permanent epidermal regeneration in patients. 4 Our results evidenced that the size of the retroviral genome does not hamper its efficient packaging into the viral capsid and generation of infectious viral particles. All three studies on transfer of collagen VII cDNA referred to above showed appropriate protein delivery at basement membrane of the dermal-epidermal junction and stable correction of the RDEB hallmarks of the reverted human skin regenerated onto grafted immunodeficient mice.…”

“…Furthermore, permanent epithelial regeneration in burn patients treated with cultured keratinocyte autografts (which are not genetically modified) evidence the persistence of functional stem cells. [2][3][4] Experimental evidence also indicated that genetic manipulation does not alter epidermal stemness. 5,6 In the past, however, the long-term persistence of the transgene expression in human keratinocytes in vivo has been difficult to obtain, and generated a long-lasting controversy.…”

Use of Autologous Skin Equivalents With Artificial Dermal Matrix (Integra) in Donor Site Coverage in Radial Forearm Free Flaps: Preliminary Cases

“…The remaining cells were frozen as previously described. 8 From a primary culture, 3 ASEs of 75 cm 2 each were obtained. To avoid keratinocyte differentiation, the submerged method was used.…”

“…Subsequently, the biopsies were processed as previously described. 8,9 Briefly, the samples were divided into small fragments and subjected to 4 washes with phosphate buffered saline 5 mL containing trypsin and ethylenediaminetetraacetic acid (Gibco, Paisley, Invitrogen, UK) at concentrations of 0.05% and 0.02%, respectively.…”

“…7 The objective of this report is to describe the preliminary cases of a novel method using an artificial dermal matrix with autologous skin equivalents (ASEs) 8,9 for the treatment of donor sites of radial forearm flaps.…”