Human Plasma ATP Concentration

Gorman, Mark W.; Feigl, Eric O.; Buffington, Charles W.

doi:10.1373/clinchem.2006.076364

Cited by 218 publications

(178 citation statements)

References 33 publications

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“…To account for ATP from red blood cell (RBC) haemolysis, a correction curve was set up as outlined previously (Gorman et al . 2007). The linear regression line of lysed RBC [ATP] (in nanomoles per litre) was plotted against the [Hb] (in milligrams per decilitre), with the relationship y = 4.61 x − 4.79 and R 2 = 0.99.…”

Section: Methodsmentioning

confidence: 99%

Mechanisms for the control of local tissue blood flow during thermal interventions: influence of temperature‐dependent ATP release from human blood and endothelial cells

Kalsi

Chiesa

Trangmar

et al. 2017

Experimental Physiology

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New Findings What is the central question of this study? Skin and muscle blood flow increases with heating and decreases with cooling, but the temperature‐sensitive mechanisms underlying these responses are not fully elucidated. What is the main finding and its importance? We found that local tissue hyperaemia was related to elevations in ATP release from erythrocytes. Increasing intravascular ATP augmented skin and tissue perfusion to levels equal or above thermal hyperaemia. ATP release from isolated erythrocytes was altered by heating and cooling. Our findings suggest that erythrocytes are involved in thermal regulation of blood flow via modulation of ATP release. Local tissue perfusion changes with alterations in temperature during heating and cooling, but the thermosensitivity of the vascular ATP signalling mechanisms for control of blood flow during thermal interventions remains unknown. Here, we tested the hypotheses that the release of the vasodilator mediator ATP from human erythrocytes, but not from endothelial cells or other blood constituents, is sensitive to both increases and reductions in temperature and that increasing intravascular ATP availability with ATP infusion would potentiate thermal hyperaemia in limb tissues. We first measured blood temperature, brachial artery blood flow and plasma [ATP] during passive arm heating and cooling in healthy men and found that they increased by 3.0 ± 1.2°C, 105 ± 25 ml min−1 °C−1 and twofold, respectively, (all P < 0.05) with heating, but decreased or remained unchanged with cooling. In additional men, infusion of ATP into the brachial artery increased skin and deep tissue perfusion to levels equal or above thermal hyperaemia. In isolated erythrocyte samples exposed to different temperatures, ATP release increased 1.9‐fold from 33 to 39°C (P < 0.05) and declined by ∼50% at 20°C (P < 0.05), but no changes were observed in cultured human endothelial cells, plasma or serum samples. In conclusion, increases in plasma [ATP] and skin and deep tissue perfusion with limb heating are associated with elevations in ATP release from erythrocytes, but not from endothelial cells or other blood constituents. Erythrocyte ATP release is also sensitive to temperature reductions, suggesting that erythrocytes may function as thermal sensors and ATP signalling generators for control of tissue perfusion during thermal interventions.

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Section: Methodsmentioning

confidence: 99%

Mechanisms for the control of local tissue blood flow during thermal interventions: influence of temperature‐dependent ATP release from human blood and endothelial cells

Kalsi

Chiesa

Trangmar

et al. 2017

Experimental Physiology

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“…Supernatants of apoptotic cells released concentrations of 100-200 nM ATP and UTP during cell death. 41,42 Studies have shown that resting ATP plasma levels are~28 nM, 73 indicating that the release of ATP from apoptotic cells is significant compared with physiological concentrations. Not surprisingly, the EC 50 for many purinergic P2Y receptors are below 1 μM, A list of knock-out mice of proteins linked to specific find-me signal release or recognition.…”

Section: Steps Involved In Clearancementioning

confidence: 99%

Do not let death do us part: ‘find-me’ signals in communication between dying cells and the phagocytes

2016

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The turnover and clearance of cells is an essential process that is part of many physiological and pathological processes. Improper or deficient clearance of apoptotic cells can lead to excessive inflammation and autoimmune disease. The steps involved in cell clearance include: migration of the phagocyte toward the proximity of the dying cells, specific recognition and internalization of the dying cell, and degradation of the corpse. The ability of phagocytes to recognize and react to dying cells to perform efficient and immunologically silent engulfment has been well-characterized in vitro and in vivo. However, how apoptotic cells themselves initiate the corpse removal and also influence the cells within the neighboring environment during clearance was less understood. Recent exciting observations suggest that apoptotic cells can attract phagocytes through the regulated release of 'find-me' signals. More recent studies also suggest that these find-me signals can have additional roles outside of phagocyte attraction to help orchestrate engulfment. This review will discuss our current understanding of the different find-me signals released by apoptotic cells, how they may be relevant in vivo, and their additional roles in facilitating engulfment.

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“…As the 'molecular unit of currency' of intracellular energy transfer, ATP transports chemical energy within cells to support metabolism 22 . ATP is present in low concentrations (o0.4 mM) in the extracellular environment, but is relatively concentrated within the intracellular cytosol (1-10 mM) [23][24][25][26] . Within cells, ATP provides energy to the cellular metabolism by breaking a phosphoanhydride bond.…”

mentioning

confidence: 99%

“…A maximum quenching efficacy was achieved when the molar ratio of duplex to Dox was 0.5. To investigate the ATP-responsive characteristics of the duplex, Dox/Duplex was incubated at 0.4 mM and 4 mM ATP concentrations, which represent the typical ATP level in the extracellular fluid and intracellular cytosol, respectively [23][24][25][26] . The dissociation of Dox/Duplex leads to the release of Dox, which can be estimated by detecting the recovery of the Dox fluorescence.…”

mentioning

confidence: 99%

ATP-triggered anticancer drug delivery

et al. 2014

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Stimuli-triggered drug delivery systems have been increasingly used to promote physiological specificity and on-demand therapeutic efficacy of anticancer drugs. Here we utilize adenosine-5'-triphosphate (ATP) as a trigger for the controlled release of anticancer drugs. We demonstrate that polymeric nanocarriers functionalized with an ATP-binding aptamer-incorporated DNA motif can selectively release the intercalating doxorubicin via a conformational switch when in an ATP-rich environment. The half-maximal inhibitory concentration of ATP-responsive nanovehicles is 0.24 mM in MDA-MB-231 cells, a 3.6-fold increase in the cytotoxicity compared with that of non-ATP-responsive nanovehicles. Equipped with an outer shell crosslinked by hyaluronic acid, a specific tumour-targeting ligand, the ATP-responsive nanocarriers present an improvement in the chemotherapeutic inhibition of tumour growth using xenograft MDA-MB-231 tumour-bearing mice. This ATP-triggered drug release system provides a more sophisticated drug delivery system, which can differentiate ATP levels to facilitate the selective release of drugs.

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Human Plasma ATP Concentration

Cited by 218 publications

References 33 publications

Mechanisms for the control of local tissue blood flow during thermal interventions: influence of temperature‐dependent ATP release from human blood and endothelial cells

Mechanisms for the control of local tissue blood flow during thermal interventions: influence of temperature‐dependent ATP release from human blood and endothelial cells

Do not let death do us part: ‘find-me’ signals in communication between dying cells and the phagocytes

ATP-triggered anticancer drug delivery

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