Human plasma fibronectin promotes proliferation and differentiation of odontoblast

Tang, Jia; Saito, Takashi

doi:10.1590/1678-7757-2016-0442

Cited by 15 publications

(14 citation statements)

References 31 publications

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“…For instance, general protocols for coating cell culture dishes require about 0.1-1 mg/mL of ECM proteins (fibronectin, laminin, Matrigel) to induce better attachment of cells. 20,21 In another study, it was found that a concentration of 3 mg/mL of ECM hydrogel was required to induce gelation in stroke cavities. 22 Thus, in routine studies, where a lot of protein is needed, the use of pure ECM proteins might be an expensive option.…”

Section: Introductionmentioning

confidence: 99%

Engineering and Functionalization of Gelatin Biomaterials: From Cell Culture to Medical Applications

Bello

Kim

et al. 2020

Tissue Engineering Part B: Reviews

399

259

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Health care and medicine were revolutionized in recent years by the development of biomaterials, such as stents, implants, personalized drug delivery systems, engineered grafts, cell sheets, and other transplantable materials. These materials not only support the growth of cells before transplantation but also serve as replacements for damaged tissues in vivo. Among the various biomaterials available, those made from natural biological sources such as extracellular proteins (collagen, fibronectin, laminin) have shown significant benefits, and thus are widely used. However, routine biomaterial-based research requires copious quantities of proteins and the use of pure and intact extracellular proteins could be highly cost ineffective. Gelatin is a molecular derivative of collagen obtained through the irreversible denaturation of collagen proteins. Gelatin shares a very close molecular structure and function with collagen and thus is often used in cell and tissue culture to replace collagen for biomaterial purposes. Recent technological advancements such as additive manufacturing, rapid prototyping, and three-dimensional printing, in general, have resulted in great strides toward the generation of functional gelatin-based materials for medical purposes. In this review, the structural and molecular similarities of gelatin to other extracellular matrix proteins are compared and analyzed. Current strategies for gelatin crosslinking and production are described and recent applications of gelatin-based biomaterials in cell culture and tissue regeneration are discussed. Finally, recent improvements in gelatin-based biomaterials for medical applications and future directions are elaborated.

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Section: Introductionmentioning

confidence: 99%

Engineering and Functionalization of Gelatin Biomaterials: From Cell Culture to Medical Applications

Bello

Kim

et al. 2020

Tissue Engineering Part B: Reviews

399

259

View full text Add to dashboard Cite

show abstract

“…With the passage of time, cells in the other concentration groups also spread, but less rapidly than those in the LN-1 (100 µg/mL) group. Early cell spreading and flattening was observed only in wells coated with 100 µg/mL LN-1, far exceeding the concentration of fibronectin (FN, 10 µg/mL) that is necessary to achieve the same effect (19). These results revealed that LN-1 is less adhesive than FN, as a significantly higher concentration of LN-1 was needed to achieve a comparable degree of cell adhesion.…”

Section: Discussionmentioning

confidence: 89%

“…In addition, we assessed the mRNA expression levels of BSP, ALP and OCN. In an earlier experiment, we had found that BSP was highly responsive to matrix proteins such as type I collagen and FN: coating with these two proteins elicited a dramatic increase of BSP expression (2-3-fold relative to the control) in MDPC-23 cells (19). In contrast, LN-1 coating of polystyrene wells resulted in only modest enhancement of BSP expression (1.15-fold in the LN-1-1 group, 1.29-fold in the LN-1-10 group, and 1.10-fold in the LN-1-100 group).…”

Section: Discussionmentioning

confidence: 94%

Laminin-1 acts as an adhesive for odontoblast-like cells and promotes their differentiation toward a hard tissue-forming phenotype

Tang

Saito

2018

J Oral Sci

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The present study was designed to investigate the effect of laminin-1 (LN-1 or LN-111) on an odontoblast-like cell line, MDPC-23. Wells of non-treated polystyrene plates were coated with various concentrations of LN-1 (0.1, 1, 10, and 100 µg/mL) and left to dry for 2 days. Water-coated surfaces were used as controls. MDPC-23 cell proliferation, differentiation and mineralization were evaluated in terms of the CCK-8 assay, ALP activity, real-time RT-PCR and Alizarin red staining. The data indicated that LN-1 promoted the proliferation of MDPC-23 cells in a concentration-dependent manner. Moreover, it enhanced ALP activity and expression of key odontogenic genes (DMP-1 and DSPP) upon addition of mineralization reagents, leading to significant promotion of calcification by the cells. These results demonstrate that LN-1 acts as an adhesive for odontoblast-like cells, allowing up-regulation of odontogenic genes and accelerating matrix mineralization. In the context of the present study, the optimal LN-1 coating concentration for MDPC-23 cells was suggested to be 100 µg/mL.

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“…As main components of the cytoskeleton, these protein structures are known to be very sensitive to any mechanical damage of the cell membrane during cryopreservation. [41] Hence, it is important that the cryopreservation protocol does not cause any disruption in their functionality, particularly resulting from osmotic stresses. [42] Apparently, in our method the random orientation of paper fibers provides protective shield to the cells to withstand the osmotic stresses from the surroundings when they undergo the harsh freezing process.…”

Section: Discussionmentioning

confidence: 99%

Paper‐Based Cell Cryopreservation

et al. 2020

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The continuous development of simple and practical cell cryopreservation methods is of great importance to a variety of sectors, especially when considering the efficient short‐ and long‐term storage of cells and their transportation. Although the overall success of such methods has been increased in recent years, there is still need for a unified platform that is highly suitable for efficient cryogenic storage of cells in addition to their easy‐to‐manage retrieval. Here, a paper‐based cell cryopreservation method as an alternative to conventional cryopreservation methods is presented. The method is space‐saving, cost‐effective, simple and easy to manage, and requires no additional fine‐tuning to conventional freezing and thawing procedures to yield comparable recovery of viable cells. It is shown that treating papers with fibronectin solution enhances the release of viable cells post thawing as compared to untreated paper platforms. Additionally, upon release, the remaining cells within the paper lead to the formation and growth of spheroid‐like structures. Moreover, it is demonstrated that the developed method works with paper‐based 3D cultures, where preformed 3D cultures can be efficiently cryopreserved.

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Human plasma fibronectin promotes proliferation and differentiation of odontoblast

Cited by 15 publications

References 31 publications

Engineering and Functionalization of Gelatin Biomaterials: From Cell Culture to Medical Applications

Engineering and Functionalization of Gelatin Biomaterials: From Cell Culture to Medical Applications

Laminin-1 acts as an adhesive for odontoblast-like cells and promotes their differentiation toward a hard tissue-forming phenotype

Paper‐Based Cell Cryopreservation

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