Human platelet lysate improves the growth and survival of cultured human pre-antral follicles

Adrados, Cristina Subiran; Zheng, Mengxue; Lund, Søren Peter; Larsen, Elisabeth; Tanvig, Mette; Greve, Vinnie Hornshøj; Blanche, Paul; Andersen, Claus Yding; Kristensen, Stine Gry

doi:10.1016/j.rbmo.2023.06.011

Cited by 7 publications

(5 citation statements)

References 35 publications

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“…Additionally, the median growth of viable follicles was significantly greater in the HPL group, although the AMH and E2 analyses did not reveal any differences between the groups. Consequently, HPL significantly enhanced the survival and growth of cultured human preantral follicles ( 49 ).…”

Section: Prp Therapy For Conditions Causing Female Infertilitymentioning

confidence: 99%

Therapeutic roles of platelet-rich plasma to restore female reproductive and endocrine dysfunction

Wang,

Li,

et al. 2024

Front. Endocrinol.

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Millions of women worldwide are infertile due to gynecological disorders, including premature ovarian insufficiency, polycystic ovary syndrome, Asherman syndrome, endometrial atrophy, and fallopian tube obstruction. These conditions frequently lead to infertility and have a substantial impact on the quality of life of the affected couples, primarily because of their psychological implications and high financial costs. Recently, using platelets to stimulate cell proliferation and tissue differentiation has emerged as a promising approach in regenerative medicine. Platelet-rich plasma (PRP) shows considerable potential for promoting endometrial hypertrophy and follicle development, making it a promising therapeutic option for tissue repair or replacement. This review provides an overview of the recent advancements and underlying mechanisms of PRP therapy for various female reproductive diseases and presents new therapeutic options for addressing female infertility.

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Section: Prp Therapy For Conditions Causing Female Infertilitymentioning

confidence: 99%

Therapeutic roles of platelet-rich plasma to restore female reproductive and endocrine dysfunction

Wang,

Li,

et al. 2024

Front. Endocrinol.

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“…Culture medium composition is pivotal to maintaining viability, growth, and proliferation of cells in an IVC system. The most commonly used basal media for this phase of human ovarian tissue IVC are alpha minimal essential medium (αMEM) [ 17 , 19 , 20 , 23 , 24 , 25 , 26 , 27 , 28 , 29 , 30 , 31 , 32 ] and McCoy’s 5a [ 21 , 33 , 34 , 35 , 36 , 37 , 38 , 39 ]. Waymouth’s medium [ 40 , 41 , 42 , 43 , 44 , 45 , 46 ] is the most widely used medium for bovine ovarian tissue culture ( Table 1 ).…”

Section: Follicle Activation: From Pmfs To Secondary Folliclesmentioning

confidence: 99%

“…This is not surprising as, during intraovarian development, follicles migrate from the rigid cortex towards the less dense medulla. Secondary follicle isolation can be performed either enzymatically [ 56 , 58 , 104 , 111 ], mechanically by microdissection [ 10 , 21 , 23 , 34 , 52 , 53 , 107 , 109 , 110 ], or a combination of both [ 39 , 105 , 106 ] ( Table 2 ). The microdissection approach, using fine-gauge needles, has been established as the most appropriate, as it maintains an intact follicle basement membrane, thereby preserving oocyte-GC communications [ 10 , 21 ].…”

Section: Follicle In Vitro Growth (Ivg): From Secondary To Antral Fol...mentioning

confidence: 99%

“…Medium composition is also crucial to IVG. The most commonly used media in human IVG systems are αMEM [ 23 , 56 , 105 , 107 , 108 ], McCoy’s 5a [ 10 , 21 , 34 , 39 ], Dulbecco’s MEM (DMEM) [ 104 ], or mixed media (DMEM+F12) [ 58 ], while McCoys’ 5a [ 109 , 110 ] and TCM-199 [ 53 ] are typically utilized in bovines ( Table 2 ).…”

Section: Follicle In Vitro Growth (Ivg): From Secondary To Antral Fol...mentioning

confidence: 99%

“…In oocytes, activin is involved in modulation of nuclear gene transcription, promoting maturation [ 119 , 120 ]. Isolated follicle growth and survival can also be enhanced with other culture additives such as bFGF [ 55 ], antioxidants like ascorbic acid that mitigate oxidative stress damage [ 21 , 47 ], and platelet-rich plasma or human platelet lysate containing high concentrations of growth factors [ 30 , 39 ]. While various research groups have tested different additives, further studies are needed to determine their effectiveness.…”

Section: Follicle In Vitro Growth (Ivg): From Secondary To Antral Fol...mentioning

confidence: 99%

See 2 more Smart Citations

Comprehensive Review of In Vitro Human Follicle Development for Fertility Restoration: Recent Achievements, Current Challenges, and Future Optimization Strategies

Vitale,

Dolmans

2024

JCM

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Ovarian tissue cryopreservation (OTC) and subsequent transplantation (OTT) is a fertility preservation technique widely offered to prepubertal girls and young fertile women who need to undergo oncological treatment but are at a high risk of infertility. However, OTT is not considered safe in patients with certain diseases like leukemia, Burkitt’s lymphoma, and ovarian cancer because of the associated risk of malignant cell reintroduction. In vitro follicle development has therefore emerged as a promising means of obtaining mature metaphase II (MII) oocytes from the primordial follicle (PMF) pool contained within cryopreserved ovarian tissue, without the need for transplantation. Despite its significant potential, this novel approach remains highly challenging, as it requires replication of the intricate process of intraovarian folliculogenesis. Recent advances in multi-step in vitro culture (IVC) systems, tailored to the specific needs of each follicle stage, have demonstrated the feasibility of generating mature oocytes (MII) from early-stage human follicles. While significant progress has been made, there is still room for improvement in terms of efficiency and productivity, and a long way to go before this IVC approach can be implemented in a clinical setting. This comprehensive review outlines the most significant improvements in recent years, current limitations, and future optimization strategies.

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Exploring the effect of platelet-rich plasma on vascularization and survival of follicles in xenotransplanted human ovarian tissue

Subiran Adrados,

Olesen,

Olesen

et al. 2024

Reproductive BioMedicine Online

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Human platelet lysate improves the growth and survival of cultured human pre-antral follicles

Cited by 7 publications

References 35 publications

Therapeutic roles of platelet-rich plasma to restore female reproductive and endocrine dysfunction

Therapeutic roles of platelet-rich plasma to restore female reproductive and endocrine dysfunction

Comprehensive Review of In Vitro Human Follicle Development for Fertility Restoration: Recent Achievements, Current Challenges, and Future Optimization Strategies

Exploring the effect of platelet-rich plasma on vascularization and survival of follicles in xenotransplanted human ovarian tissue

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