2014
DOI: 10.3389/fphar.2014.00150
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Human pluripotent stem cells on artificial microenvironments: a high content perspective

Abstract: Self-renewing stem cell populations are increasingly considered as resources for cell therapy and tools for drug discovery. Human pluripotent stem (hPS) cells in particular offer a virtually unlimited reservoir of homogeneous cells and can be differentiated toward diverse lineages. Many diseases show impairment in self-renewal or differentiation, abnormal lineage choice or other aberrant cell behavior in response to chemical or physical cues. To investigate these responses, there is a growing interest in the d… Show more

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Cited by 12 publications
(8 citation statements)
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References 146 publications
(166 reference statements)
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“…Cell behaviour is heavily influenced by genetics and by the surrounding environment [14] , [15] . In order to evaluate specific differences on cell behaviour, we reasoned that diverse coating concentrations on multiwell plates could be exploited.…”
Section: Resultsmentioning
confidence: 99%
“…Cell behaviour is heavily influenced by genetics and by the surrounding environment [14] , [15] . In order to evaluate specific differences on cell behaviour, we reasoned that diverse coating concentrations on multiwell plates could be exploited.…”
Section: Resultsmentioning
confidence: 99%
“…The overall result is a reliable, efficient (85%–95% purity), and relatively inexpensive human PSC cardiomyocyte differentiation protocol using E8 and CDM3 media with predictable maturation and subtype specification. Other known alternatives to mTeSR1 include StemPro (Invitrogen), 38 Pluripro (Cell Guidance Systems), 3941 PluriSTEM (Millipore), 41,42 and Nutristem (Stemgent) 41,43 ; however, not all of these media have been tested for cardiac differentiation.…”
Section: Psc Monolayer-based Differentiationmentioning
confidence: 99%
“…Examples of commercially available chemically-defined media for a feeder-free (FF) culture of human iPSCs are PluriSTEM (Merck Millipore, Darmstadt, Germany), StemPro (Invitrogen, Carlsbad, CA, USA), mTeSR1 (STEMCELL Technologies, Vancouver, BC, Canada), Pluripro (Cell Guidance Systems, St. Louis, MO, USA), Stemline (Sigma, St. Louis, MO, USA), and Essential-8 (Invitrogen, Carlsbad, CA, USA) [ 10 ]. Despite all the advantages in terms of expansion efficiency, the long-term clinical side-effects on human beings in the case of SC transplantation needs to be assessed in advance.…”
Section: Introductionmentioning
confidence: 99%
“…A “smart” 3-D substrate, may be able to instruct cells towards the right fate, limiting and, whenever possible, avoiding any medium additive or supplement and/or xenogeneic-factor. There would be enormous benefits in terms of safety and risk mitigation during in vitro manipulation, thus leading to a potential industrial-scale expansion of cells for therapeutic purposes [ 10 ].…”
Section: Introductionmentioning
confidence: 99%