Human Polyomaviruses and Other Human Viruses in Neuroendocrine Tumors

Schmitt, Markus; Höfler, Daniela; Koleganova, Nadezda; Pawlita, Michael

doi:10.1158/1055-9965.epi-11-0424

Cited by 23 publications

(20 citation statements)

References 28 publications

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“…The differences in the detection rates may be explained in part by geographic epidemiological variations in patients with NSCLCs or merely by the technical approaches used. The absence of MCPyV we found in LCNECs and mesotheliomas is consistent with previous reports (Busam et al , 2009; Duncavage et al , 2009; Bhatia et al , 2010; Schmitt et al , 2011). Moreover, our results do not support a direct association between MCPyV and thymoma.…”

Section: Discussionsupporting

confidence: 93%

“…Two German groups showed the presence of MCPyV DNA in a subset of SCLCs, with detection rates of 6.7% (2 out of 30) and 38.9% (7 out of 18; Andres et al , 2009; Helmbold et al , 2009). In contrast, no evidence of a causal relationship between MCPyV and pulmonary LCNEC has been reported (Busam et al , 2009; Duncavage et al , 2009; Schmitt et al , 2011). The prevalence of MCPyV in non-small cell lung cancers (NSCLCs) has not been investigated well.…”

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confidence: 97%

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Detection of Merkel cell polyomavirus with a tumour-specific signature in non-small cell lung cancer

et al. 2013

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Background:We searched for a viral aetiology for non-small cell lung cancer (NSCLC), focusing on Merkel cell polyomavirus (MCPyV).Methods:We analysed 112 Japanese cases of NSCLC for the presence of the MCPyV genome and the expressions of RNA transcripts and MCPyV-encoded antigen. We also conducted the first analysis of the molecular features of MCPyV in lung cancers.Results:PCR revealed that 9 out of 32 squamous cell carcinomas (SCCs), 9 out of 45 adenocarcinomas (ACs), 1 out of 32 large-cell carcinomas, and 1 out of 3 pleomorphic carcinomas were positive for MCPyV DNA. Some MCPyV DNA-positive cancers expressed large T antigen (LT) RNA transcripts. Immunohistochemistry showed that MCPyV LT antigen was expressed in the tumour cells. The viral integration sites were identified in one SCC and one AC. One had both episomal and integrated/truncated forms. The other carried an integrated MCPyV genome with frameshift mutations in the LT gene.Conclusion:We have demonstrated the expression of a viral oncoprotein, the presence of integrated MCPyV, and a truncated LT gene with a preserved retinoblastoma tumour-suppressor protein-binding domain in NSCLCs. Although the viral prevalence was low, the tumour-specific molecular signatures support the possibility that MCPyV is partly associated with the pathogenesis of NSCLC in a subset of patients.

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Section: Discussionsupporting

confidence: 93%

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confidence: 97%

Detection of Merkel cell polyomavirus with a tumour-specific signature in non-small cell lung cancer

et al. 2013

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“…In total, 37 and 38 formalin-fixed paraffin-embedded, mucosal melanoma samples [45,55], 25 fresh-frozen, central nervous system tumours, 31 fresh-frozen, neuroblastoma samples [46], 50 neonatal, dried blood spots from children who developed later acute lymphoblastic leukaemia [50], 43 formalin-fixed paraffin-embedded neuroendocrine tumour samples [48], 30 fresh lung adenocarcinomas [49] and 32 neuroendocrine lung tumours [47] were found to be negative for KIPyV and WUPyV DNA by standard [45,46,47], nested [50], real-time PCR [49] and polyomavirus genotyping assay [48]. Babakir-Mina et al [63] detected the VP1 region of KIPyV in 9 out of 40 fresh lung cancer tissue samples, although only 2 malignant tissue samples were positive for the early region of KI virus.…”

Section: Resultsmentioning

confidence: 99%

“…Some publications about the prevalence of KIPyV, WUPyV and HPyV9 in different tumours are available, but these viruses have not been hitherto examined in bladder and renal cancers [45,46,47,48,49,50,51,52,53,54,55,56,57]. In the present study the prevalence of KIPyV, WUPyV, HPyV9 and BKPyV were examined by PCR in tissue samples from the urinary tract.…”

Section: Introductionmentioning

confidence: 92%

No Evidence of Human Polyomavirus 9, WU and KI DNA in Kidney and Urinary Bladder Tumour Tissue Samples

Csoma¹,

Bidiga

Méhes

et al. 2016

Pathobiology

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Background/Aims: The oncogenic potential of human polyomaviruses (HPyVs) has been proposed, but so far only Merkel cell carcinoma polyomavirus seems to be associated with a human tumour. The role of BK polyomavirus (BKPyV) in human tumourigenesis remains controversial. BKPyV establishes persistent infection in the urinary tract, and renal and bladder neoplasms have been studied extensively, but conflicting prevalence data are reported. KI, WU and HPyV9 were detected in urine samples suggesting that these viruses may also infect the urinary tract, but their presence in urinary tract tumours has not been studied. The aim of this work was to examine the prevalence of KIPyV, WUPyV, HPyV9 and BKPyV by PCR in renal and bladder neoplasms. Methods: A total of 190 formalin-fixed paraffin-embedded renal neoplasms, bladder cancer and kidney biopsy samples were analysed for the presence of BKPyV, KIPyV, WUPyV and HPyV9 DNA by real-time and nested PCR. Results: Amplifiable DNA was extracted from all the samples, but none of the studied viruses were detected in benign renal neoplasia (0/23), malignant renal tumours (0/89) or bladder cancer (0/76). Conclusion: Our study did not find any evidence that BKPyV, KIPyV, WUPyV or HPyV9 are associated with bladder and renal tumours.

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“…Over the last years, we have already developed several multiplex PCR followed by bead-based Luminex detection using specific probes to detect multiple agents simultaneously in a high-throughput fashion, e.g. human papillomaviruses [27], [28], bovine papillomaviruses [29], human polyomaviruses [30], adeno-associated viruses [31] and cell culture contaminations [32].…”

Section: Introductionmentioning

confidence: 99%

A Bead-Based Multiplex Assay for the Detection of DNA Viruses Infecting Laboratory Rodents

et al. 2014

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The Federation of European Laboratory Animal Science Association (FELASA) recommends screening of laboratory rodents and biological materials for a broad variety of bacterial agents, viruses, and parasites. Methods commonly used to date for pathogen detection are neither cost-effective nor time- and animal-efficient or uniform. However, an infection even if silent alters experimental results through changing the animals’ physiology and increases inter-individual variability. As a consequence higher numbers of animals and experiments are needed for valid and significant results. We developed a novel high-throughput multiplex assay, called rodent DNA virus finder (rDVF) for the simultaneous identification of 24 DNA viruses infecting mice and rats. We detected all 24 DNA viruses with high specificity and reproducibility. Detection limits for the different DNA viruses varied between 10 and 1000 copies per PCR. The validation of rDVF was done with DNA isolated from homogenised organs amplified by pathogen specific primers in one multiplex PCR. The biotinylated amplicons were detected via hybridisation to specific oligonucleotide probes coupled to spectrally distinct sets of fluorescent Luminex beads. In conclusion, rDVF may have the potential to replace conventional testing and may simplify and improve routine detection of DNA viruses infecting rodents.

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Human Polyomaviruses and Other Human Viruses in Neuroendocrine Tumors

Cited by 23 publications

References 28 publications

Detection of Merkel cell polyomavirus with a tumour-specific signature in non-small cell lung cancer

Detection of Merkel cell polyomavirus with a tumour-specific signature in non-small cell lung cancer

No Evidence of Human Polyomavirus 9, WU and KI DNA in Kidney and Urinary Bladder Tumour Tissue Samples

A Bead-Based Multiplex Assay for the Detection of DNA Viruses Infecting Laboratory Rodents

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