Human red blood cell stroma: an alternative to traditional allogeneic adsorption methods

Abstract

ABBREVIATIONS: DAT = direct antiglobulin test; EGA = ethylenediaminetetraacetate glycine acid; IAT = indirect antiglobulin test; PEG = polyethylene glycol; wAIHA = warm autoantibody immune hemolytic anemia. From the

Daudi cell stroma: An alternative to dithiothreitol to resolve daratumumab interference in pretransfusion testing

“…However, the efficiency of the adsorption was related to the number of Daudi cells used, suggesting the existence of a threshold above which the number of cells used is high enough to provide a sufficient number of CD38 molecules to allow binding to DARA and result in an efficient depletion. 17 Wilson and Branch 10 have recently shown that human RBC stroma prepared using digitonin is an efficient and effective method to remove autoantibody reactivity and allow detection of potentially clinically significant antibodies. In light of that study, and the demonstration of an effective DARA depletion using intact Daudi cells, we have prepared Daudi cell stroma using a sonication based method.…”

“…Furthermore, Daudi stroma adsorption has additional advantages such as reduced dilutional effects due to efficient packing of the stromal adsorption material and potentially long shelf life, because it can be frozen for months, as demonstrated by previous studies that used human stroma. 10 However, this method requires special equipment such as ultrasonic processor and tabletop centrifuge with performance up to 20 000g, which could impact its broader use.…”

“…Cell stroma from different sources has been used in adsorption studies. 9,10 Since cell stroma could provide large quantities of adsorption material that can be stored frozen and used when needed, Daudi cells stroma was generated and tested as DARA adsorption reagent. The use of Daudi cells stroma revealed an effective and significant adsorption of DARA (Table 1).…”