Human red blood cells express the RNA sensor TLR7 and bind viral RNA

Lam, LK Metthew; Clements, Rebecca L; Eckart, Kaitlyn; Weisman, A.R.; Vaughan, Andy E.; Yehya, Nadir; Meyer, Nuala J.; Jurado, Kellie Ann; Mangalmurti, Nilam S.

doi:10.1101/2022.01.01.474694

Cited by 2 publications

(3 citation statements)

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“…TLR7 находятся на внешней мембране эритроцитов и ассоциированы с белком полосы 3. Эритроциты связывают РНК из патогенных одноцепочечных РНК-вирусов [101]. SARS-CoV-2 содержит несколько одноцепочечных фрагментов РНК, которые могут быть распознаны TLR7 и TLR8 [102].…”

Section: протромботические изменения эритроцитов при коронавирусной и...unclassified

“…TLR7 взаимодействуют с мембранным белком полосы 3 эритроцитов, и это связывание усиливается при острой инфекции SARS-CoV-2. Также отмечено выявление взаимодействия спай-белка вируса с эритроцитами [101]. Белок полосы 3 соединяет липидный бислой с нижележащим мембранным белковым скелетом, анкирином, а также поддерживает анионный обмен (HCO 3 и Cl -) между клеткой и плазмой крови.…”

Section: протромботические изменения эритроцитов при коронавирусной и...unclassified

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Structural and functional changes in erythrocytes associated with the development of thrombosis and disorders of hemostasis

Кручинина

Gromov

Kruchinina

2022

jour

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The data of clinical and experimental studies of the last decade force us to reconsider the role of red blood cells in the development of thrombosis and disorders of the hemostasis system. This area is of particular relevance during the COVID-19 pandemic. The purpose of the review is to present data on the structural and functional changes in erythrocytes, on changes in their number associated with the development of thrombosis and hemostasis disorders, including in patients with coronavirus infection. The article presents the results of scientific research devoted to the study of morphological and functional changes in red blood cells that affect hemostasis and thrombosis. These changes include erythrocyte number or hematocrit (which modulates blood rheology through blood viscosity) and qualitative changes such as deformability, erythrocyte aggregation, expression of adhesive proteins and phosphatidylserine, release of extracellular microvesicles, hemolysis, piezo receptor status, modulation of nitric oxide bioavailability. Changes in the parameters of erythrocytes that occur during blood storage are shown, with possible thrombotic consequences during blood transfusion. The most recent research concerns changes in red blood parameters during SARS-CoV-2 infection that are associated with the development of thrombosis: virus attachment and amplification of viral proteins in erythropoiesis progenitor cells; activation of stress erythropoiesis with an increase in nuclear erythrocyte cell percentage up to 42 %; activation of the processes of oxidation of the protein band 3 with its excessive cleavage, oxidation and cleavage of the alpha-chain of spectrin, ankyrin; changes in the lipid architecture of the membrane and a decrease in the activity of erythrocyte antioxidants, which mediate disturbances in cell deformability and impaired ATP release; reducing the ability of red blood cells to secrete nitric oxide; decrease in the level of sphingolipids of the erythrocyte membrane; excessive production of microvesicles with tissue factor; an increase in erythrocyte rigidity with impaired release of intraerythrocyte nitric oxide due to SARS-CoV-2 virus attacking the hemoglobin 1-beta chain and porphyrin capture with potential heme inhibition; an increase on the surface of erythrocytes expression of activated complement components C3b and C4d, immunoglobulin IgG, which worsens cell deformability; attachment of erythrocytes through the Toll-like receptor 9 to neutrophil extracellular traps, which contributes to thrombosis; increased presentation of phosphatidylcholine on erythrocyte membranes, which facilitates the assembly of the tenase and prothrombinase complexes, contributing to the production of thrombin, an increase in intracellular calcium levels with stimulation of the formation of microvesicles with prothrombotic potential. The presented data indicate a significant role of changes in erythrocyte parameters in the development of hemostasis disorders, especially during the COVID-19 pandemic, which makes red blood cells a target for therapy and suggests a change in the emphasis of management tactics for patients with thrombosis.

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Section: протромботические изменения эритроцитов при коронавирусной и...unclassified

Structural and functional changes in erythrocytes associated with the development of thrombosis and disorders of hemostasis

Кручинина

Gromov

Kruchinina

2022

jour

View full text Add to dashboard Cite

show abstract

“…TLR7 взаимодействует с мембранным белком полосы 3 эритроцитов, и это взаимодействие усиливается при острой инфекции SARS-CoV-2. Также отмечено связывание спайкового белка вируса с эритроцитами [34]. Белок полосы 3 соединяет липидный бислой с нижележащим мембранным белковым скелетом, анкирином.…”

Section: Introductionunclassified

Changes in red blood cells associated with the development of cardiovascular complications in patients with COVID-19 coronavirus infection

Кручинина

Gromov

Logvinenko

et al. 2023

jour

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The purpose of the review is to highlight the most significant changes in the parameters of red blood cells associated with the development of thrombosis in patients with coronavirus infection. A search was carried out using keywords in the databases Scopus, Web of Science, PubMed according to literary sources of the last 3 years on changes in erythrocyte indices associated with thrombosis against the background of COVID-19 infection. Information is presented on the main shifts in red blood indicators during SARS-CoV-2 infection associated with the development of thrombosis: virus attachment and amplification of viral proteins in erythropoiesis progenitor cells; activation of stress erythropoiesis with an increase in nuclear erythrocyte cell content up to 45 %; activation of band 3 protein oxidation with its excessive cleavage, oxidation and cleavage of alpha-chains of spectrin, ankyrin; changes in the lipid architecture of the membrane and a decrease in the activity of erythrocyte antioxidant activity, which mediate violations of cell deformability and impaired release of ATP; a decrease in the ability of erythrocytes to secrete nitric oxide; a decrease in the level of sphingolipids of the erythrocyte membrane; excessive production of microvesicles with tissue factor; an increase in the rigidity of erythrocytes with impaired release of intra-erythrocyte nitric oxide due to an attack by the SARS-CoV-2 virus 1-hemoglobin beta chain and porphyrin capture with potential heme inhibition; an increase in activated complement components C3b and C4d, immunoglobulin IgG expression on erythrocyte surface, which worsens cell deformability; attachment of erythrocytes through Toll-like receptor 9 to neutrophil extracellular traps, which promotes thrombosis; increased presentation of phosphatidylcholine on erythrocyte membranes, which facilitates the assembly of the tenase complex and prothrombinase complex, contributing to the production of thrombin, an increase in intracellular calcium levels with stimulation of the formation of microvesicles with prothrombotic potential; activation of oxidative stress in erythrocytes under conditions of hypoxia with generation of reactive oxygen species, hemoglobin autooxidation. Conclusions. The data obtained indicate the active role of erythrocytes in the development of intravascular disorders and microcirculation disorders with the risk of cardiovascular complications in patients with COVID-19. Probably, the involvement of red blood cells causes the development of systemic hypoxia in those patients. A detailed study of the identified shifts makes it possible to identify new targets for therapy and improve the prognosis of patients with COVID-19.

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Human red blood cells express the RNA sensor TLR7 and bind viral RNA

Cited by 2 publications

References 42 publications

Structural and functional changes in erythrocytes associated with the development of thrombosis and disorders of hemostasis

Structural and functional changes in erythrocytes associated with the development of thrombosis and disorders of hemostasis

Changes in red blood cells associated with the development of cardiovascular complications in patients with COVID-19 coronavirus infection

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