Human rhabdomyosarcoma cells express functional pituitary and gonadal sex hormone receptors: Therapeutic implications

Poniewierska-Baran, Agata; Schneider, Gabriela; Sun, Wenlu; Abdelbaset‐Ismail, Ahmed; Barr, Frederic G.; Ratajczak, Mariusz Z.

doi:10.3892/ijo.2016.3439

Cited by 14 publications

(12 citation statements)

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“…All hormones were prepared according to the manufacturer’s recommendations. Lyophilisates were dissolved in the substances recommended in the product specification [ 24 – 26 ] and based on previous experience [ 21 , 22 , 27 ] and then added to the culture medium:…”

Section: Methodsmentioning

confidence: 99%

“…Similarly, functional expression of FSH and LH receptors in established breast cancer cell lines has shown that sex hormones (SexHs) regulate breast cancer cell motility, adhesion, and invasion [ 20 ]. Moreover, functional receptors for pituitary gonadotropins and gonadal SexHs were identified on the surface of human lung cancer cells [ 16 ], rhabdomyosarcoma cells [ 21 ], and leukemia cells [ 22 ].…”

Section: Introductionmentioning

confidence: 99%

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A novel potential role of pituitary gonadotropins in the pathogenesis of human colorectal cancer

et al. 2018

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BackgroundColorectal cancer (CRC) is a leading cause of death in the western world, and its incidence increases with patient age. It is also known that with age there occur changes in the levels of certain hormones, including an increase in the secretion of pituitary gonadotropins (PtGs) as a result of the loss of gonadal hormone feedback. We recently reported that functional PtG receptors are expressed in human lung cancer cells, rhabdomyosarcoma cells, and malignant hematopoietic stem cells.FindingsHere we report for the first time that the receptors for follicle-stimulating hormone (FSH) and luteinizing hormone (LH) are expressed in primary tumor samples isolated from CRC patients as well as in the established human CRC cell lines HTC116 and HTB37. Moreover, we also report that PtGs stimulate chemotaxis, adhesion, and proliferation of these cell lines.ConclusionsOur results suggest that PtGs play an important and underappreciated role in CRC pathogenesis, and we call for further studies to better define their role in gastrointestinal malignancies and their direct effect on putative CRC cancer stem cells.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

A novel potential role of pituitary gonadotropins in the pathogenesis of human colorectal cancer

et al. 2018

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“…RMS can be divided into two histological subtypes: embryonal rhabdomyosarcoma (ERMS) and alveolar rhabdomyosarcoma (ARMS). ARMS is associated with more aggressive behavior which has a worse prognosis than ERMS [ 18 ].…”

Section: Introductionmentioning

confidence: 99%

Recent advancements in the use of exosomes as drug delivery systems

et al. 2018

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Extracellular vesicles (EVs) are the substances that are released by most types of cells and have an important role in cell to cell communication. Among the most highly researched EVs are exosome. Recent studies show that exosomes derived from cells have different roles and targets. Many studies show that exosome can efficiently deliver many different kinds of cargo to the target cell. Therefore, they are often used to deliver therapeutic cargo for treatment. The exosomes that have been used include both natural ones and those that have been modified with other substances to increase the delivery ability. This article provides a review of both exosomes derived from various cells and modified exosome and their ability in delivering the many kinds of cargo to the target cell.

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“…Evidence has accumulated that several types of malignancies share certain markers with germ cells and respond to stimulation by SexHs (1,4–6). In support of this connection, some tumors express pluripotency markers (e.g., Oct-4), secrete carcinoembryonic antigen (CEA), express cancer-testis antigens (CTAs), and respond by proliferation after stimulation by both pituitary and gonadal SexHs (26–30).…”

Section: Discussionmentioning

confidence: 99%

“…Evidence has accumulated that sex hormones (SexHs) play a role in the development and progression of several malignancies arising in the gonads (1), urogenital tract (2–4), breast (5), skeletal muscles (6) and hemato/lymphohematopoietic tissues (7). Gonadal SexHs, and in particular pituitary SexHs such as follicle-stimulating hormone (FSH), luteinizing hormone (LH), and prolactin (PRL), are potent mitogens, and their enhanced level in peripheral blood (PB) is associated with prostate, colon, and lung cancer (8–11).…”

Section: Introductionmentioning

confidence: 99%

Pituitary sex hormones enhance the pro-metastatic potential of human lung cancer cells by downregulating the intracellular expression of heme oxygenase-1

Abdelbaset‐Ismail

Pędziwiatr

Schneider

et al. 2016

International Journal of Oncology

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We report that human lung cancer cell lines express functional receptors for pituitary sex hormones (SexHs) and respond to stimulation by follicle-stimulating hormone (FSH), luteinizing hormone (LH), and prolactin (PRL). Expression of these receptors has also been confirmed in patient lung cancer samples at the mRNA level. Stimulation of human lung cancer cell lines with FSH, LH, or PRL stimulated migration and chemotaxis, and some cell lines responded by enhanced proliferation. Moreover, priming of human lung cancer cells by exposing them to pituitary SexHs resulted in enhanced seeding efficiency of injected human lung cancer cells into bone marrow, liver, and lungs in an immunodeficient mouse model. The chemotaxis of lung cancer cell lines corresponded with the activity of heme oxygenase-1 (HO-1), as stimulation of these cells by FSH, LH, and PRL downregulated its expression in a p38 MAPK-dependent manner. Moreover, while downregulation of HO-1 by the small-molecule inhibitor tin protoporphyrin (SnPP) promoted migration, upregulation of HO-1 by the small-molecule activator cobalt protoporphyrin (CoPP) showed the opposite effect. Based on this finding, we propose that pituitary SexHs play a significant role in the pathogenesis of lung cancer, particularly when the blood level of FSH increases due to gonadal dysfunction with advanced age. Finally, we propose that upregulation of HO-1 expression by a small-molecule activator may be effective in controlling SexH-induced cell migration in lung cancer.

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Human rhabdomyosarcoma cells express functional pituitary and gonadal sex hormone receptors: Therapeutic implications

Cited by 14 publications

References 50 publications

A novel potential role of pituitary gonadotropins in the pathogenesis of human colorectal cancer

A novel potential role of pituitary gonadotropins in the pathogenesis of human colorectal cancer

Recent advancements in the use of exosomes as drug delivery systems

Pituitary sex hormones enhance the pro-metastatic potential of human lung cancer cells by downregulating the intracellular expression of heme oxygenase-1

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