Human Rotavirus Type 2: Cultivation in Vitro

Abstract

A strain of type 2 human rotavirus (Wa) was grown to relatively high titer through 14 passages in primary cultures of African green monkey kidney (AGMK) cells. This passage series was initiated with virus that had been passaged 11 times serially in newborn gnotobiotic piglets. In contrast, virus present in the stool of patient Wa as well as virus from the first, second, or third passage in piglets could not be propagated successfully in African green monkey kidney cells. Prior to each passage in cell culture, the virus was treated with trypsin and the inoculated cultures were centrifuged at low speed. Cultivation of a type 2 human rotavirus should aid attempts to characterize this virus and to develop a means of immunoprophylaxis for a serious diarrheal disease of human infants.

Diarrhoea in Mice Infected with a Human Rotavirus

“…Similar studies now underway revealed that the Wa strain, serotype 1, subgroup II of human rotavirus (Wyatt et al, 1980) and the human-bovine reassortant virus DSI made by H. B.…”

Chemical disinfection of human rotavirus-contaminated inanimate surfaces

“…The Wa strain of human rotavirus (Wyatt et al 1980) and the MA-104 line of embryonic rhesus monkey kidney cells were used throughout this study. The techniques used for cell culture and virus growth ) and virus quantitation (Raphael, Sattar & Springthorpe, 1985) (NBC).…”

Isolation and Characterization of Anti-Rotavirus Immunoglobulins Secreted by Cloned Hybridoma Cell Lines

“…Additional studies were aimed at comparing the reactivity of the hybridoma preparations with other rotaviruses, namely the human Wa strain (Wyatt et al, 1980) and the simian rotavirus (SAI 1). As shown in Fig.…”