Human salivary gland morphogenesis: myoepithelial cell maturation assessed by immunohistochemical markers

Ianez, Renata F; Buim, Marcilei Eliza Cavicchioli; Coutinho‐Camillo, Cláudia Malheiros; Schultz, Regina; Soares, Fernando Augusto; Lourenço, Sílvia Vanessa

doi:10.1111/j.1365-2559.2010.03645.x

Cited by 49 publications

(54 citation statements)

References 32 publications

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“…The immunohistochemical profiles of nonneoplastic and neoplastic myoepithelial cells have been documented in several studies. 12,16,17 Consistent with these reports, S100 and Glial fibrillary acidic protein, classical myoepithelial cell markers, which are similar to SOX10, exhibit low sensitivity and specificity for myoepithelial cells. SOX10 sensitivity to myoepithelial cells most closely resembles Smooth muscle actin and calponin, which are often used as sensitive and specific markers.…”

Section: Discussionmentioning

confidence: 61%

“…Among these markers, only Glial fibrillary acidic protein was not expressed in normal myoepithelial cells, but was expressed in neoplastic myoepithelial cells, in agreement with previous data. 12 Furthermore, CK5/6 and CK14 were expressed in the cytoplasm of most (490%) abluminal cells of acini to excretory ducts, and few (o5%) of the luminal cells of excretory ducts (data not shown). The present results suggest that SOX10 is a useful marker to identify acini.…”

Section: Discussionmentioning

confidence: 86%

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SOX10 is a novel marker of acinus and intercalated duct differentiation in salivary gland tumors: a clue to the histogenesis for tumor diagnosis

Mori²,

et al. 2013

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Salivary gland tumors are relatively rare and morphologically diverse and heterogeneous tumors; therefore, histogenesis-based tumor markers are sorely needed to aid in diagnosing and determining the cell type of origin. SRY-related HMG-box 10 (SOX10) protein is a transcription factor known to be crucial in the specification of the neural crest and maintenance of Schwann cells and melanocytes. In addition, positive expression has also been implicated in the major salivary gland. Here, we examined SOX10 expression in various salivary gland tumors to correlate this expression with myoepithelial markers. Overall, 76 malignant and 14 benign tumors were examined. SOX10 expression clearly delineated two distinct subtypes of human salivary gland tumors; acinic cell carcinomas, adenoid cystic carcinomas, epithelial-myoepithelial carcinomas, myoepithelial carcinomas, and pleomorphic adenomas, including the pleomorphic adenoma component of carcinoma, were SOX10 positive, while salivary duct carcinomas, mucoepidermoid carcinomas, an oncocytic carcinoma, Oncocytomas, and Warthin tumors were SOX10 negative. Also, SOX10 was expressed in solid-type or nonspecific morphology salivary gland tumors, but was not expressed in poorly differentiated squamous cell carcinomas. In normal human salivary gland tissue, SOX10 expression was specific to the nuclei of acini and both luminal and abluminal cells of intercalated ducts but not in other sites. Moreover, the murine model suggested that SOX10 continued to be expressed from the developmental stage to adulthood in the acinar and both luminal and abluminal intercalated ducts in the major salivary gland. Thus, SOX10 is a novel marker for diagnosing and understanding the histogenesis of salivary gland tumors.

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Section: Discussionmentioning

confidence: 61%

Section: Discussionmentioning

confidence: 86%

SOX10 is a novel marker of acinus and intercalated duct differentiation in salivary gland tumors: a clue to the histogenesis for tumor diagnosis

Mori²,

et al. 2013

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“…While some degree of overlap was observed between E14.5 and E16.5 in processes involving cell differentiation and organ development, we did identify unique biological functions related to muscle function at E16.5. Interestingly, we found specific enrichment in pathways involving muscle structure, muscle tissue development and regulation of muscle contraction, properties intimately associated with functions of the myoepithelial cells [41, 42] (Fig. 3c).…”

Section: Resultsmentioning

confidence: 99%

RNA-seq based transcriptomic map reveals new insights into mouse salivary gland development and maturation

et al. 2016

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BackgroundMouse models have served a valuable role in deciphering various facets of Salivary Gland (SG) biology, from normal developmental programs to diseased states. To facilitate such studies, gene expression profiling maps have been generated for various stages of SG organogenesis. However these prior studies fall short of capturing the transcriptional complexity due to the limited scope of gene-centric microarray-based technology. Compared to microarray, RNA-sequencing (RNA-seq) offers unbiased detection of novel transcripts, broader dynamic range and high specificity and sensitivity for detection of genes, transcripts, and differential gene expression. Although RNA-seq data, particularly under the auspices of the ENCODE project, have covered a large number of biological specimens, studies on the SG have been lacking.ResultsTo better appreciate the wide spectrum of gene expression profiles, we isolated RNA from mouse submandibular salivary glands at different embryonic and adult stages. In parallel, we processed RNA-seq data for 24 organs and tissues obtained from the mouse ENCODE consortium and calculated the average gene expression values. To identify molecular players and pathways likely to be relevant for SG biology, we performed functional gene enrichment analysis, network construction and hierarchal clustering of the RNA-seq datasets obtained from different stages of SG development and maturation, and other mouse organs and tissues. Our bioinformatics-based data analysis not only reaffirmed known modulators of SG morphogenesis but revealed novel transcription factors and signaling pathways unique to mouse SG biology and function. Finally we demonstrated that the unique SG gene signature obtained from our mouse studies is also well conserved and can demarcate features of the human SG transcriptome that is different from other tissues.ConclusionsOur RNA-seq based Atlas has revealed a high-resolution cartographic view of the dynamic transcriptomic landscape of the mouse SG at various stages. These RNA-seq datasets will complement pre-existing microarray based datasets, including the Salivary Gland Molecular Anatomy Project by offering a broader systems-biology based perspective rather than the classical gene-centric view. Ultimately such resources will be valuable in providing a useful toolkit to better understand how the diverse cell population of the SG are organized and controlled during development and differentiation.Electronic supplementary materialThe online version of this article (doi:10.1186/s12864-016-3228-7) contains supplementary material, which is available to authorized users.

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“…Detection of myoepithelial differentiation both in aspiration cytology and histologic examination can also help diagnosing a tumor as of salivary gland type [12]. The proteins SMA, calponin, CD29, S100, and P63, which are present from the earliest stage of maturation of myoepithelial cell are the most valuable for their diagnosis [13]. On immunohistochemistry, in our case tumor was positive for CK, EMA, S-100, and p63 and negative for SMA, thus proving the case as pleomorphic adenoma with predominantly myoepithelial cells type.…”

Section: Discussionmentioning

confidence: 99%

Myoepithelial Cell-Rich Pleormorphic Adenoma of Minor Salivary Gland of Parapharyngeal Space

Rawat

Sonkhya

et al. 2012

Case Reports in Otolaryngology

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Parapharyngeal space tumors are rare and constitute only 0.5–1.0% of head and neck tumors. Minor salivary gland tumor is still rare in parapharyngeal space. We are reporting a case of pleomorphic adenoma of minor salivary gland of parapharyngeal space. A 42-year-old female presented with a history of mass in the oropharynx for 3 years. She presented with “hot potato voice” and dysphagia. CECT and MRI were done, showing large parapharyngeal space tumor. FNAC was suspicious for tumor of nerve cell origin. Tumor was excised using “paramedian mandibulotomy with mandibular swing approach”. Histopathological examination was inconclusive, suggesting possibility of extraskeletal myxoid chondrosarcoma, solitary fibrous tumor, neurogenic tumor. On immunohistochemistry, tumor was positive for cytokeratin, EMA (dim), S-100, and P 63 and negative for SMA thus proving the case as myoepithelial cell-rich pleomorphic adenoma.

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Human salivary gland morphogenesis: myoepithelial cell maturation assessed by immunohistochemical markers

Abstract: The proteins SMA, calponin, CD29, S100 and p63, which are present from the earliest stages of salivary gland maturation, are valuable myoepithelial markers but, although very specific, are not exclusive markers for this cell type.

Cited by 49 publications

References 32 publications

SOX10 is a novel marker of acinus and intercalated duct differentiation in salivary gland tumors: a clue to the histogenesis for tumor diagnosis

SOX10 is a novel marker of acinus and intercalated duct differentiation in salivary gland tumors: a clue to the histogenesis for tumor diagnosis

RNA-seq based transcriptomic map reveals new insights into mouse salivary gland development and maturation

Myoepithelial Cell-Rich Pleormorphic Adenoma of Minor Salivary Gland of Parapharyngeal Space

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