Human serum IgA downregulates the release of inflammatory cytokines (tumor necrosis factor-alpha, interleukin-6) in human monocytes

112

We have studied the effect of the probiotic strain Lactobacillus plantarum 299v on the immune functions of gnotobiotic rats. One group of germ-free rats was colonized with the type 1-fimbriated Escherichia coli O6:K13:H1 and another group with the same E. coli strain together with L. plantarum 299v. One and 5 weeks after colonization, bacterial numbers were determined in the contents of the small intestine, caecum and mesenteric lymph nodes. Small intestinal sections were examined for CD8+, CD4+, CD25+ (IL-2R alpha-chain), IgA+ and MHC class II+ cells and mitogen-induced spleen cell proliferation was determined. Immunoglobulin levels and E. coli-specific antibodies were measured in serum. Rats given L. plantarum in addition to E. coli showed lower counts of E. coli in the small intestine and caecum 1 week after colonization compared with the group colonized with E. coli alone, but similar levels after 5 weeks. Rats colonized with L. plantarum + E. coli had significantly higher total serum IgA levels and marginally higher IgM and IgA antibody levels against E. coli than those colonized with E. coli alone. They also showed a significantly increased density of CD25+ cells in the lamina propria and displayed a decreased proliferative spleen cell response after stimulation with concanavalin A or E. coli 1 week after colonization. The results indicate that L. plantarum colonization competes with E. coli for intestinal colonization and can influence intestinal and systemic immunity.

Section: Discussionmentioning

confidence: 99%

Immunomodulatory effects ofLactobacillus plantarumcolonizing the intestine of gnotobiotic rats

Herías

Hessle

Telemo

et al. 1999

112

“…The induction of cytokine release was based on the method described by Wolf et al . [28]. The adherent monocytes were incubated overnight in RPMI-AB medium to reduce background nonspecific cytokine production and subsequently washed two times.…”

Section: Stimulation Of Peripheral Mononuclear Cells and Monocytesmentioning

confidence: 99%

Immunomodulatory properties of human serum immunoglobulin A: anti-inflammatory and pro-inflammatory activities in human monocytes and peripheral blood mononuclear cells

Olas

Butterweck

Teschner

et al. 2005

SummaryOur study investigated the immunomodulatory activities of human plasmaderived serum immunoglobulin (Ig)A. Previous findings seem contradictory indicating either pro-or anti-inflammatory activities. We used serum IgA purified from large plasma pools and studied the modulation of the release of cytokines and chemokines from resting and lipopolysaccharide ( We conclude that human serum IgA has both an anti-inflammatory and a pro-inflammatory capacity and this dual capacity might contribute to the feedback mechanisms maintaining a balance between pro-inflammatory and anti-inflammatory activities.

“…Human mononuclear cells (MNC) were isolated from heparinized peripheral blood of healthy adult blood donors as previously described [16]. For induction of cytokine release, 1 2 10 6 MNC/ ml per well were incubated for 24 h in the presence of phorbol 12myristate 13-acetate (PMA; Sigma Chemical Co., St Louis, MO), final concentration 100 ng/ml (0 .…”

Section: Induction Of Cytokine Releasementioning

confidence: 99%

“…162 mol/l ), purified lipopolysaccharide (LPS; prepared from Escherichia coli serotype 0111:B4 by phenol extraction; Sigma, no. L-2630, final concentration 10 ng/ ml), heat-inactivated encapsulated Haemophilus influenzae type b (Hib; strain Eagan, kindly provided by Dr G. Zerlauth, Immuno AG; final concentration 1 2 10 6 bacteria/ml), purified human serum IgA, or medium alone (complete medium [16], containing 10% pooled, heat-inactivated (30 min, 568C) fetal calf serum (FCS; HyClone Labs, Logan, UT)). Adherent human monocytes were prepared and cytokine release was stimulated after overnight culture in complete medium as previously described [16].…”

Section: Induction Of Cytokine Releasementioning

confidence: 99%

Anti-inflammatory properties of human serum IgA: induction of IL-1 receptor antagonist and FcαR (CD89)-mediated down-regulation of tumour necrosis factor-alpha (TNF-α) and IL-6 in human monocytes

Wolf

Hauber

Gulle

et al. 1996

Self Cite

SUMMARYA deregulated expression and/or release of large amounts of inflammatory cytokines such as IL-1 and TNF-® accounts for most pathophysiological events in a variety of systemic inflammatory diseases, the effect being mediated by the interaction of these cytokines with their respective receptors. IL-1 receptor antagonist (IL-1Ra), mainly produced by monocytes/macrophages, is an inhibitor of IL-1 activity. The present study shows that human serum IgA induces significant IL-1Ra release in human peripheral blood mononuclear cells and adherent monocytes. IgA induced higher levels of IL-1Ra than Haemophilus influenzae type b (Hib) expressing lipopolysaccharide (LPS), purified LPS or phorbol myristate acetate (PMA), without induction of IL-1¯release, and even inhibited LPS-induced IL-1r elease. Induction of IL-1Ra by IgA could be detected both at the mRNA and protein levels in resting and activated monocytes. Ligation of Fc®R with MoAb MY-43 or treatment with human serum IgA induced protein tyrosine phosphorylation in human monocytes, and herbimycin A, a specific inhibitor of protein tyrosine kinase activity, inhibited IgA-induced IL-1Ra production, suggesting that Fc®R-mediated induction of tyrosine phosphorylation is required for the IgA-induced stimulation of IL-1Ra release. In addition, triggering of Fc®R with MoAb specifically down-regulated TNF-® and IL-6 release in human monocytes activated with Hib. By the induction of IL-1Ra and down-regulation of the release of inflammatory cytokines such as IL-1¯, TNF-® and IL-6, interaction of IgA with human monocytes may actively contribute to the regulation of the inflammatory response.