Human serum IgE‐mediated mast cell degranulation shows poor correlation to allergen‐specific IgE content

Marchand, Françoise; Mécheri, Salaheddine; Guilloux, L.; Iannascoli, Bruno; Weyer, A.; Blank, Ulrich

doi:10.1034/j.1398-9995.2003.00251.x

Cited by 57 publications

(51 citation statements)

References 24 publications

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“…To characterize saliva bioactivity, mast cells were incorporated into the minifeeders at the concentration of 10 6 /ml in Tyrode buffer and were exposed to mosquito bites (100 female mosquitoes) for 2 h. As controls, minifeeders containing the same mast cell preparations were kept out of reach of mosquitoes. Cells were harvested, and after centrifugation, supernatants were analyzed for their content in ␤-hexosaminidase as described previously (28). All results are expressed as a percentage of total ␤-hexosaminidase in the cells after correction for spontaneous release in unstimulated cultures (net release).…”

Section: Mast Cell Degranulation Assaymentioning

confidence: 99%

Anopheles Mosquito Bites Activate Cutaneous Mast Cells Leading to a Local Inflammatory Response and Lymph Node Hyperplasia

Demeure¹,

Brahimi

Hacini³

et al. 2005

The Journal of Immunology

128

116

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When Anopheles mosquitoes probe the skin for blood feeding, they inject saliva in dermal tissue. Mosquito saliva is known to exert various biological activities, but its perception by the immune system and its role in parasite transmission remain poorly understood. In the present study, we report on the cellular changes occurring in the mouse skin and draining lymph nodes after a Anopheles stephensi mosquito bite. We show that mosquito bites induce dermal mast cell degranulation, leading to fluid extravasation and neutrophil influx. This inflammatory response does not occur in mast cell-deficient W/Wv mice, unless these are reconstituted specifically with mast cells. Mast cell activation caused by A. stephensi mosquito bites is followed by hyperplasia of the draining lymph node due to the accumulation of CD3+, B220+, CD11b+, and CD11c+ leukocytes. The T cell enrichment of the draining lymph nodes results from their sequestration from the circulation rather than local proliferation. These data demonstrate that mosquito bites and very likely saliva rapidly trigger the immune system, emphasizing the critical contribution of peripheral mast cells in inducing T cell and dendritic cell recruitment within draining lymph nodes, a prerequisite for the elicitation of T and B lymphocyte priming.

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Section: Mast Cell Degranulation Assaymentioning

confidence: 99%

Anopheles Mosquito Bites Activate Cutaneous Mast Cells Leading to a Local Inflammatory Response and Lymph Node Hyperplasia

Demeure¹,

Brahimi

Hacini³

et al. 2005

The Journal of Immunology

128

116

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“…Dibbern et al (2003) showed that the absolute amount of peanut-specific IgE is an important determinant of the ability of a serum to effectively sensitize cells for allergen-dependent degranulation (r ¼ 0.95; p50.001), but that the amount of total IgE was a relatively poor predictor. Conversely, Marchand et al (2003) indicated no correlation (r ¼ 0.27) between the proportion of bound allergen-specific IgE and strength of the degranulation response after sensitization with serum IgE. However, a significant correlation (r ¼ 0.97) was found for purified monoclonal IgE.…”

Section: Rat Basophil Leukemia (Rbl) Cell Modelmentioning

confidence: 81%

“…Incubation with undiluted serum or 420% serum was found to cause cytotoxic effects (i.e. decrease viability) in the RBL cells (Ladics et al, 2008;Marchand et al, 2003). This toxicity appears to be due to naturally occurring xenoreactive antibodies (Palmera et al, 2005;Schuurman et al, 2003).…”

Section: Rat Basophil Leukemia (Rbl) Cell Modelmentioning

confidence: 99%

“…Therefore, RBL cell activation mediated through serum IgE is a result of complex relationships not only dependent on allergenspecific IgE content, but also the capacity to efficiently sensitize cells and trigger signaling responses that lead to degranulation. Further, some serum factors may hamper binding of IgE to F ce RI expressed on the cell membrane and adverse effects on the cells occur (Dibbern et al, 2003;Marchand et al, 2003;Vogel et al, 2006). Those factors and how they may act on cells to interfere with the assay have not yet been specifically identified.…”

Section: Rat Basophil Leukemia (Rbl) Cell Modelmentioning

confidence: 99%

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Use of a rat basophil leukemia (RBL) cell-based immunological assay for allergen identification, clinical diagnosis of allergy, and identification of anti-allergy agents for use in immunotherapy

Sun

Zhou

Zhou³

et al. 2014

Journal of Immunotoxicology

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(2015) Use of a rat basophil leukemia (RBL) cell-based immunological assay for allergen identification, clinical diagnosis of allergy, and identification of anti-allergy agents for use in immunotherapy, Journal of Immunotoxicology, 12:2, 199-205, DOI: 10.3109/1547691X.2014 AbstractFood allergy is an important public health problem that affects an estimated 8% of young children and 2% of adults. With an increasing interest in genetically-engineered foods, there is a growing need for development of sensitive and specific tests to evaluate potential allergenicity of foods and novel proteins as well as to determine allergic responses to ensure consumer safety. This review covers progress made in the field of development of cell models, specifically that involving a rat basophil leukemia (RBL) cell-based immunoassay, for use in allergen identification, diagnosis, and immunotherapy. The RBL assay has been extensively employed for determining biologically relevant cross-reactivities of food proteins, assessing the effect of processing on the allergenicity of food proteins, diagnosing allergic responses to whole-food products, and identifying anti-allergy food compounds. From the review of the literature, one might conclude the RBL cell-based assay is a better test system when compared to wild-type mast cell and basophil model systems for use in allergen identification, diagnosis, and analyses of potential immunotherapeutics. However, it is important to emphasize that this assay will only be able to identify those allergens to which the human has already been exposed, but will not identify a truly novel allergen, i.e. one that has never been encountered as in its preferred (humanized) configuration.

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“…Upon activation by the high-affinity IgE receptors (FcRI), they release factors such as histamine, cytokines, chemokines that ultimately cause allergic responses (1,2). Gab2 is a downstream molecule of FcRI and plays an important role in FcRI-mediated signaling events in mast cells.…”

Section: Introductionmentioning

confidence: 99%

Inhibition of the Antigen-Induced Activation of RBL-2H3 Cells by Gab2 siRNA

Huang¹,

Tong²,

Deng³

et al. 2008

Cell Mol Immunol

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Human serum IgE‐mediated mast cell degranulation shows poor correlation to allergen‐specific IgE content

Cited by 57 publications

References 24 publications

Anopheles Mosquito Bites Activate Cutaneous Mast Cells Leading to a Local Inflammatory Response and Lymph Node Hyperplasia

Anopheles Mosquito Bites Activate Cutaneous Mast Cells Leading to a Local Inflammatory Response and Lymph Node Hyperplasia

Use of a rat basophil leukemia (RBL) cell-based immunological assay for allergen identification, clinical diagnosis of allergy, and identification of anti-allergy agents for use in immunotherapy

Inhibition of the Antigen-Induced Activation of RBL-2H3 Cells by Gab2 siRNA

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