Human specific antigens and species difference in non-hemoglobin proteins of red cell lysate.

Abstract

Non-hemoglobin proteins (NHPs) of 12 species of mammalian were effectively isolated from red cell lysates with CM-Sephadex chromatography. Polyacrylamide gel disc electrophoresis demonstrated that human and monkey NHPs had 13 and 11 bands, respectively, but guinea pig and swine two to three bands. Rabbit anti-human NHP reacted with monkey, bovine, canine and badger as well as human. Absorption of the anti-NHP with monkey NHP resulted in preparing human specific anti-NHP. The specific antiserum gave two bands locating near the origin in immunoelectrophoresis. Human specific antigens were prepared by affinity chromatography with the anti-NHP. The antigens were composed of three bands, two of which had slightly faster mobility than carbonic anhydrase and the other migrated fastly on disc electrophoresis. These bands were proved to have neither LDH nor esterase activity.hemolysate; non-hemoglobin protein; human specific antigen; species difference

An esterase isozyme in non-hemoglobin proteins of cell lysate reacting with mushroom lectin.

“…Elution with the buffer gave three peaks, of which the first two peaks were designated as NHP. Anti-NHP was raised in rabbit as described previously (Sagisaka et al 1981b).…”