Human sperm cells express CD44

Bains, R.; Adeghe, J. H.; Carson, R.J.

doi:10.1016/s0015-0282(02)03230-2

Cited by 31 publications

(20 citation statements)

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“…a v ß 3 , ß 1 subunit) may cooperate in adhesion, signaling pathways, and stimulation of motility in various cell types (Chellaiah et al 2003, Gao et al 2003, Marroquin et al 2004, Zhu et al 2004. CD44 is expressed on the acrosomal region of human sperm cells (Bains et al 2002), on porcine oocytes and cumulus cells (Yokoo et al 2002), and bovine oocytes and early embryos (Furnus et al 2003). Sperm-associated OPN may interact with CD44 on bovine oocytes during fertilization to facilitate adhesion and signaling.…”

Section: Identified Opnmentioning

confidence: 99%

Detection of osteopontin on Holstein bull spermatozoa, in cauda epididymal fluid and testis homogenates, and its potential role in bovine fertilization

et al. 2007

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Osteopontin (OPN) is a secreted extracellular matrix phosphoprotein identified in various tissues and fluids including those of the male and female reproductive tracts. OPN was previously identified as a 55 kDa high fertility marker in Holstein bull seminal plasma, produced by the ampulla and the vesicular gland. The objectives of this study were to characterize OPN on ejaculated and cauda epididymal sperm using immunofluorescence and western blot analysis, and to assess the role of sperm OPN in fertilization. Solubilized sperm membrane proteins from ejaculated and cauda epididymal sperm were separated by 1D SDS-PAGE, transferred to nitrocellulose, and probed with an antibody to bovine milk OPN. A 35 kDa protein was detected by this antibody in both ejaculated and cauda epididymal sperm membranes. Analyses also recognized OPN at 55 and 25 kDa in cauda epididymal fluid and testicular parenchyma homogenates respectively. Immunofluorescent analysis of ejaculated and cauda epididymal sperm showed OPN localization in a well-defined band in the postacrosomal region of the sperm head and also on the midpiece. Results of in vitro fertilization experiments showed that sperm treated with an antibody to OPN fertilized fewer oocytes than sperm treated with control medium while increasing incidence of polyspermy, suggesting a role of sperm-associated OPN in fertilization and a block to polyspermy. These studies demonstrate that OPN exists at multiple molecular weight forms in the bull reproductive tract and its presence on ejaculated sperm may signal its importance in fertilization by interacting with integrins or other proteins on the oocyte plasma membrane. Reproduction (2007) 133 909-917

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Section: Identified Opnmentioning

confidence: 99%

Detection of osteopontin on Holstein bull spermatozoa, in cauda epididymal fluid and testis homogenates, and its potential role in bovine fertilization

et al. 2007

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“…Initially, microscopy was used to detect hyaladherins, including CD44 on human sperm, which was detected on the acrosome as has been reported elsewhere (Bains et al, 2002) and equatorial regions of washed spermatozoa. Compared with CD44, however, the signals obtained with a fluorescently tagged probe for hyaluronic-acid-binding (HA-TRITC), capable of generically recognizing hyaladherins (including CD44) seemed to be more widely distributed over the sperm surface with some localized areas of high intensity, particularly in the neck region.…”

Section: Changes In Sperm Hyaluronic-acid-binding and Hyperactive Motmentioning

confidence: 99%

“…These results confirm previous reports that hyaladherins (CD44 among them) are present on human sperm (Kornovski et al, 1994;Cherr et al,. 2001;Bains et al, 2002;Kim et al, 2008;Martin-Deleon, 2011). The failure to completely abolish labelling of hyaladherins after cold incubation with hyaluronic acid probably reflects the transient interaction of hyaluronic acid with its receptors compared with the stronger and more stable interaction between antibodies and their ligands (Plazinski and Knys-Dzieciuch, 2012).…”

Section: Changes In Sperm Hyaluronic-acid-binding and Hyperactive Motmentioning

confidence: 99%

“…CD44 (Bains et al, 2002) or facilitate sperm penetration of the cumulus layers via a hyaluronidase activity followed by accessing and binding to the zona pellucida, e.g. SPAM1 (McLeskey et al, 1998) Processing crude semen by DDGC works by favouring the sedimentation of mature sperm with higher density and motility into and through the denser layer(s) of a gradient (Sakkas et al., 2000;Sakkas, 2013).…”

Section: Introductionmentioning

confidence: 99%

mentioning

confidence: 99%

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Sedimentation properties in density gradients correspond with levels of sperm DNA fragmentation, chromatin compaction and binding affinity to hyaluronic acid

Torabi

Binduraihem

Miller

2017

Reproductive BioMedicine Online

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ReuseUnless indicated otherwise, fulltext items are protected by copyright with all rights reserved. The copyright exception in section 29 of the Copyright, Designs and Patents Act 1988 allows the making of a single copy solely for the purpose of non-commercial research or private study within the limits of fair dealing. The publisher or other rights-holder may allow further reproduction and re-use of this version -refer to the White Rose Research Online record for this item. Where records identify the publisher as the copyright holder, users can verify any specific terms of use on the publisher's website. TakedownIf you consider content in White Rose Research Online to be in breach of UK law, please notify us by emailing eprints@whiterose.ac.uk including the URL of the record and the reason for the withdrawal request. Accepted ManuscriptTitle: Sedimentation properties in density gradients correspond with levels of sperm DNA fragmentation, chromatin compaction and binding affinity to hyaluronic acid 22-11-2016 Please cite this article as: Forough Torabi, Adel Binduraihem, David Miller, Sedimentation properties in density gradients correspond with levels of sperm DNA fragmentation, chromatin compaction and binding affinity to hyaluronic acid, Reproductive BioMedicine Online (2016), http://dx.doi.org/doi: 10.1016/j.rbmo.2016.11.011. This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. AbstractMature spermatozoa bind hyaluronic acid in the extracellular matrix via hyaladherins.Immature spermatozoa may be unable to interact because they do not express the appropriate hyaladherins on their surface. Fresh human semen samples were fractionated using differential density gradient centrifugation (DDGC) and the ability of these fractions to bind hyaluronic acid was evaluated. The presence of sperm hyaladherins was also assessed. CD44 was located mainly on the acrosome and equatorial segment and became more restricted to the equatorial segment in capacitated spermatozoa. Hyaluronic acid-TRITC (hyaluronan acid conjugated with tetramethylrhodamine isothiocyanante), a generic hyaluronic-acid-binding reagent, labelled the membrane and the neck region, particularly after capacitation. Sperm populations obtained after DDGC or after interaction with hyaluronic acid were assessed for DNA fragmentation and chromatin maturity. Strong relationships between both measures and sperm sedimentation and hyaluronic-acid-binding profiles were revealed. Capacitation Overall success rates for assisted conception procedures, however (including ICSI), have remained relatively static, with about 24% of cycles for all age groups deliverin...

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The Synthetic and Secretory Behaviors (Nonsteroidal) of Ovarian Follicular Granulosa Cells: Parallels to Cells of the Endothelial Cell Lineage

Antczak

2004

Essential IVF

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Human sperm cells express CD44

Cited by 31 publications

References 20 publications

Detection of osteopontin on Holstein bull spermatozoa, in cauda epididymal fluid and testis homogenates, and its potential role in bovine fertilization

Detection of osteopontin on Holstein bull spermatozoa, in cauda epididymal fluid and testis homogenates, and its potential role in bovine fertilization

Sedimentation properties in density gradients correspond with levels of sperm DNA fragmentation, chromatin compaction and binding affinity to hyaluronic acid

The Synthetic and Secretory Behaviors (Nonsteroidal) of Ovarian Follicular Granulosa Cells: Parallels to Cells of the Endothelial Cell Lineage

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