Human Spermatids and Spermatozoa Lacking Acrosomes

Holstein, A. F.; Schirren, C.; Schirren, Carl Georg

doi:10.1530/jrf.0.0350489

Cited by 80 publications

(44 citation statements)

References 3 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Thus, the intimate relationship between the acrosome and nucleus is essential for spermatid nuclear elongation or sperm head formation, since both the absence of the acrosome and a failure of nuclear elongation are simultaneously found in round-headed spermatozoa in human globozoospermia (Schirren et al, 1971;Holstein et al, 1973;Baccetti et al, 1977;Nistal et al, 1978;Escalier 1990). In a sequence of GOPC -/ -spermatid deformations, the acrosome formation failure preceded the nuclear deformation, as evidenced in a previous paper (Yao et al, 2002) and in this study showing that, when the nuclear shape is still round in step 1-8 spermatids, the acrosome formation has already been impaired (Fig.…”

Section: Discussionmentioning

confidence: 99%

Failure to assemble the peri-nuclear structures in GOPC deficient spermatids as found in round-headed spermatozoa

Ito

Suzuki-Toyota

Maekawa

et al. 2004

Archives of Histology and Cytology

View full text Add to dashboard Cite

formed, and did not always associate with developing spermatids. Consequently, spermatid nuclear elongation to form round-headed spermatozoa developed was impaired.In addition to these sequential failures, the posterior ring deficiency was attributed to the tail deformation destined to occur during epididymal maturation as reported in an accompanying paper (Suzuki-Toyota et al., 2004 in this issue), its eventual phenotype being reminiscent of the round-headed spermatozoa of human infertile globozoospermia.

show abstract

Section: Discussionmentioning

confidence: 99%

Failure to assemble the peri-nuclear structures in GOPC deficient spermatids as found in round-headed spermatozoa

Ito

Suzuki-Toyota

Maekawa

et al. 2004

Archives of Histology and Cytology

View full text Add to dashboard Cite

show abstract

“…acrosomal aplasia or agenesis, hence the synonym round-headed sperm. 54 Globozoospermia is the morphological end result of disturbed spermiogenesis and, recently, genetic etiologies have been described. 55,56 Various case reports have detailed the birth of ICSI-conceived offspring using round-headed acrosomeless spermatozoa, but in consecutive case series, the results after ICSI are poor and unpredictable, even when artificial oocyte activation with calcium ionophore is applied.…”

Section: Absolute Indications For Icsi In Male Factor Infertilitymentioning

confidence: 99%

Male factor infertility and ART

Tournaye¹

2011

Asian J Androl

View full text Add to dashboard Cite

“…Consequently, the epididymis of Fads2À/À is filled with sloughed round spermatids and a small number of sperm that exhibit globozoospermia, a condition resulting in sperm with abnormal round heads [18]. Impairment of acrosome formation during spermiogenesis leads to globozoospermia [19]. Thus, the presence of globozoospermia in Fads2À/À mice suggests that DHA may be required for acrosome formation.…”

Section: Introductionmentioning

confidence: 99%

Deficiency in the Omega-3 Fatty Acid Pathway Results in Failure of Acrosome Biogenesis in Mice1

Roqueta‐Rivera

Abbott

Sivaguru

et al. 2011

Biology of Reproduction

View full text Add to dashboard Cite

An omega-3 fatty acid, docosahexaenoic acid (DHA), is enriched in testicular membrane phospholipids, but its function is not well understood. The Fads2 gene encodes an enzyme required for the endogenous synthesis of DHA. Using Fads2-null mice (Fads2-/-), we found in our preceding studies that DHA deficiency caused the arrest of spermiogenesis and male infertility, both of which were reversed by dietary DHA. In this study, we investigated a cellular mechanism underlying the DHA essentiality in spermiogenesis. Periodic acid-Schiff staining and acrosin immunohistochemistry revealed the absence of acrosomes in Fads2-/- round spermatids. Acrosin, an acrosomal marker, was scattered throughout the cytoplasm of the Fads2-/- spermatids, and electron microscopy showed that proacrosomal granules were formed on the trans-face of the Golgi. However, excessive endoplasmic reticulum and vesicles were present on the cis-face of the Golgi in Fads2-/- spermatids. The presence of proacrosomal vesicles but lack of a developed acrosome in Fads2-/- spermatids suggested failed vesicle fusion. Syntaxin 2, a protein involved in vesicle fusion, colocalized with acrosin in the acrosome of wild-type mice. In contrast, syntaxin 2 remained scattered in reticular structures and showed no extensive colocalization with acrosin in the Fads2-/- spermatids, suggesting failed fusion with acrosin-containing vesicles or failed transport and release of syntaxin 2 vesicles from Golgi. Dietary supplementation of DHA in Fads2-/- mice restored an intact acrosome. In conclusion, acrosome biogenesis under DHA deficiency is halted after release of proacrosomal granules. Misplaced syntaxin 2 suggests an essential role of DHA in proper delivery of membrane proteins required for proacrosomal vesicle fusion.

show abstract

Human Spermatids and Spermatozoa Lacking Acrosomes

Cited by 80 publications

References 3 publications

Failure to assemble the peri-nuclear structures in GOPC deficient spermatids as found in round-headed spermatozoa

Failure to assemble the peri-nuclear structures in GOPC deficient spermatids as found in round-headed spermatozoa

Male factor infertility and ART

Deficiency in the Omega-3 Fatty Acid Pathway Results in Failure of Acrosome Biogenesis in Mice1

Contact Info

Product

Resources

About