Human stem cell research and regenerative medicine--present and future

Volarević, Vladislav; Ljujić, Biljana; Stojković, Petra; Lukić, Aleksandra; Arsenijević, Nebojša; Stojković, Miodrag

doi:10.1093/bmb/ldr027

Cited by 98 publications

(78 citation statements)

References 36 publications

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“…Technique of obtaining iPSCs opens new and exciting possibilities in medical research, especially personalized cell therapies for the therapy of various diseases (80). To treat damaged tissues or repair organs in elderly patients, it will be necessary to establish iPSCs from their This article has been peer-reviewed and accepted for publication, but has yet to undergo copyediting and proof correction.…”

Section: Aging Of Induced Pluripotent Stem Cellsmentioning

confidence: 99%

Aging of Stem and Progenitor Cells: Mechanisms, Impact on Therapeutic Potential, and Rejuvenation

Nurković

Volarević

Lako

et al. 2016

Rejuvenation Research

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It was once suggested that adult or tissue-specific stem cells may be immortal; however, several recently published data suggest that their efficacy is limited by natural aging in common with most other somatic cell types. Decreased activity of stem cells in old age raises questions as to whether the age of the donor should be considered during stem cell transplantation and at what age the donor stem cells should be harvested to ensure the largest possible number of viable, functional, and non-altered stem cells. Although stem cells remain active into old age, changes in stem cells and their microenvironments inhibit their regenerative potential. The impact of aging on stem cell populations differs between tissues and depends on a number intrinsic and extrinsic factors, including systemic changes associated with immune system alterations. In this review, we describe key mechanisms of stem and progenitor cell aging and techniques that are currently used to identify signs of stem cells aging. Furthermore, we focus on the impact of aging on the capacity for proliferation, differentiation, and clinical use of stem cells. Finally, we detail the aging of embryonic, mesenchymal, and induced pluripotent stem cells, with particular emphasis on aging mechanisms and rejuvenation.

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Section: Aging Of Induced Pluripotent Stem Cellsmentioning

confidence: 99%

Aging of Stem and Progenitor Cells: Mechanisms, Impact on Therapeutic Potential, and Rejuvenation

Nurković

Volarević

Lako

et al. 2016

Rejuvenation Research

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“…These cells are characterized by unlimited self-renewal and the ability to undergo differentiation into all cell lineages, such as cardiomyocytes (Doss et al, 2008), hepatocytes (Ochiya et al, 2010), adipocytes , and germ cells (Zhou et al, 2010), making them an attractive resource for the generation of cells that could be used for cellbased transplantation therapies (Intawicha et al, 2009;Volarevic et al, 2011). The ability to maintain ESCs in long-term culture provides a time window to modify the genome of these cells by homologous recombination (HR) (e.g., gene knockout).…”

mentioning

confidence: 99%

Recombinant Rabbit Leukemia Inhibitory Factor and Rabbit Embryonic Fibroblasts Support the Derivation and Maintenance of Rabbit Embryonic Stem Cells

Chen

Zhang

et al. 2012

Cellular Reprogramming

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The rabbit is a classical experimental animal species. A major limitation in using rabbits for biomedical research is the lack of germ-line-competent rabbit embryonic stem cells (rbESCs). We hypothesized that the use of homologous feeder cells and recombinant rabbit leukemia inhibitory factor (rbLIF) might improve the chance in deriving germ-line-competent rbES cells. In the present study, we established rabbit embryonic fibroblast (REF) feeder layers and synthesized recombinant rbLIF. We derived a total of seven putative rbESC lines, of which two lines (M5 and M23) were from culture Condition I using mouse embryonic fibroblasts (MEFs) as feeders supplemented with human LIF (hLIF) (MEF + hLIF). Another five lines (R4, R9, R15, R21, and R31) were derived from Condition II using REFs as feeder cells supplemented with rbLIF (REF + rbLIF). Similar derivation efficiency was observed between these two conditions (8.7% vs. 10.2%). In a separate experiment with 2 · 3 factorial design, we examined the effects of feeder cells (MEF vs. REF) and LIFs (mLIF, hLIF vs. rbLIF) on rbESC culture. Both Conditions I and II supported satisfactory rbESC culture, with similar or better population doubling time and colony-forming efficiency than other combinations of feeder cells with LIFs. Rabbit ESCs derived and maintained on both conditions displayed typical ESC characteristics, including ESC pluripotency marker expression (AP, Oct4, Sox2, Nanog, and SSEA4) and gene expression (Oct4, Sox2, Nanog, c-Myc, Klf4, and Dppa5), and the capacity to differentiate into three primary germ layers in vitro. The present work is the first attempt to establish rbESC lines using homologous feeder cells and recombinant rbLIF, by which the rbESCs were derived and maintained normally. These cell lines are unique resources and may facilitate the derivation of germline-competent rbESCs.

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“…In the postnatal and adult stages of life, tissue-specific stem cells are found in differentiated organs and are instrumental in repair following injury to the organ. The major characteristics of stem cells are (a) self-renewal (the ability to extensively proliferate), (b) clonality (usually arising from a single cell), and (c) potency (the ability to differentiate into different cell types) [7, 8]. Totipotent or omnipotent cells are the most undifferentiated cells and are found in early development.…”

Section: Stem Cells: a Novel Hope In Cell-based Therapymentioning

confidence: 99%

Stem Cells as New Agents for the Treatment of Infertility: Current and Future Perspectives and Challenges

Volarević

Bojić

Nurković

et al. 2014

BioMed Research International

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Stem cells are undifferentiated cells that are present in the embryonic, fetal, and adult stages of life and give rise to differentiated cells that make up the building blocks of tissue and organs. Due to their unlimited source and high differentiation potential, stem cells are considered as potentially new therapeutic agents for the treatment of infertility. Stem cells could be stimulated in vitro to develop various numbers of specialized cells including male and female gametes suggesting their potential use in reproductive medicine. During past few years a considerable progress in the derivation of male germ cells from pluripotent stem cells has been made. In addition, stem cell-based strategies for ovarian regeneration and oocyte production have been proposed as future clinical therapies for treating infertility in women. In this review, we summarized current knowledge and present future perspectives and challenges regarding the use of stem cells in reproductive medicine.

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Human stem cell research and regenerative medicine--present and future

Abstract: A number of new strategies become very important in regenerative medicine. However, we discuss the limitations of stem cells and latest development in the reprogramming research.

Cited by 98 publications

References 36 publications

Aging of Stem and Progenitor Cells: Mechanisms, Impact on Therapeutic Potential, and Rejuvenation

Aging of Stem and Progenitor Cells: Mechanisms, Impact on Therapeutic Potential, and Rejuvenation

Recombinant Rabbit Leukemia Inhibitory Factor and Rabbit Embryonic Fibroblasts Support the Derivation and Maintenance of Rabbit Embryonic Stem Cells

Stem Cells as New Agents for the Treatment of Infertility: Current and Future Perspectives and Challenges

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