Human T lymphocyte clones specific for malaria (Plasmodium falciparum) antigens.

Sinigaglia, Francesco; Richard, J.-M.; Pink, L.

doi:10.1002/j.1460-2075.1985.tb04153.x

Cited by 34 publications

(19 citation statements)

References 17 publications

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“…Nevertheless, CD8 1 T cells specific for malaria antigens are activated during bloodstage malaria [10,11]. Taken together with our observation that the numbers of activated CD8 1 T cells increased after infection with PyL, antigens derived from malaria parasites must be presented on MHC class I molecules on APC including macrophages.…”

Section: Discussionmentioning

confidence: 59%

“…However, some studies have stressed the existence of CD8 1 T cells specific for blood-stage malaria parasites. First, CD8 1 T-cell clones that proliferate and produce IFN-g in response to blood-stage malarial antigens in an HLA-restricted manner are generated in patients who live in a malaria-endemic area [11]. Second, experimental infections of non-immune volunteers with an ultra-low dose of infected RBC induce immunity to subsequent challenge in the absence of detectable antibody responses.…”

Section: Introductionmentioning

confidence: 99%

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Involvement of CD8⁺ T cells in protective immunity against murine blood‐stage infection with Plasmodium yoelii 17XL strain

et al. 2010

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When developing malaria vaccines, the most crucial step is to elucidate the mechanisms involved in protective immunity against the parasites. We found that CD8+ T cells contribute to protective immunity against infection with blood‐stage parasites of Plasmodium yoelii. Infection of C57BL/6 mice with P. yoelii 17XL was lethal, while all mice infected with a low‐virulence strain of the parasite 17XNL acquired complete resistance against re‐infection with P. yoelii 17XL. However, the host mice transferred with CD8+ T cells from mice primed only with P. yoelii 17XNL failed to acquire protective immunity. On the other hand, the irradiated host mice were completely resistant to P. yoelii 17XL infection, showing no grade of parasitemia when adoptively transferred with CD8+ T cells from immune mice that survived infection with both P. yoelii XNL and, subsequently, P. yoelii 17XL. These protective CD8+ T cells from immune WT mice had the potential to generate IFN‐γ, perforin (PFN) and granzyme B. When mice deficient in IFN‐γ were used as donor mice for CD8+ T cells, protective immunity in the host mice was fully abrogated, and the immunity was profoundly attenuated in PFN‐deficient mice. Thus, CD8+ T cells producing IFN‐γ and PFN appear to be involved in protective immunity against infection with blood‐stage malaria.

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Section: Discussionmentioning

confidence: 59%

Section: Introductionmentioning

confidence: 99%

Involvement of CD8⁺ T cells in protective immunity against murine blood‐stage infection with Plasmodium yoelii 17XL strain

et al. 2010

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“…We have earlier reported that both CD4' and CD8' P.5-specific TLC could be obtained from an acutely infected patient [9]. In this study TLC from another acutely infected and 4 clinically immune donors were analyzed.…”

Section: Phenotype Of Tlc From Acutely Infected or Immune Donorsmentioning

confidence: 99%

“…PBMC from the patients were transformed with EBV as described [9]. Briefly, lo7 PBMC were resuspended in 10 ml RPMI-FCS containing 30% supernatant of the EBV-producing marmoset cell line B95.8 and 600 nglml cyclosporin A (Sandoz, Basel, Switzerland) and distributed in the wells of a flat-bottom 96-well microculture plate at 5 X 104/well.…”

Section: Establishment Of Epstein-barr Virus (Ebv)-transformed Cell Lmentioning

confidence: 99%

Plasmodium falciparum‐specific human T cell clones: evidence for helper and cytotoxic activities

et al. 1987

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This report describes the isolation, and the phenotypic and functional characterization of Plasmodium falciparum-specific human T lymphocyte clones (TLC) obtained from 2 acutely infected and 4 clinically immune donors. Approximately one third of the TLC obtained from the acutely infected patients had the phenotype CD8+/CD4-. No such clones were obtained from the clinically immune donors. P. falciparum-specific, major histocompatibility complex-restricted CD8+ clones can lyse unrelated tumor cell targets in the presence of anti-CD3 antibodies, suggesting that these clones have cytotoxic potential. Conversely no killing was obtained with two CD4+ P. falciparum-specific TLC, even in the presence of anti-CD3 antibody, In addition the helper function of a CD4+ clone was demonstrated in a T/B cell cooperation system.

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“…ELISA tests specific for IFN-a or IFN-p [16] [20] T lymphocyte clones were stimulated with the respective antigens under the conditions described, and culture supernatants were harvested 48 h later.…”

Section: Measurement Of Ifn Avamentioning

confidence: 99%

Identity between human interferon‐γ and “macrophage‐activating factor” produced by human T lymphocytes

et al. 1986

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Human peripheral blood monocytes purified by counterflow elutriation were activated in vitro by human natural or recombinant interferon-gamma (IFN-gamma) as shown by enhanced killing of Listeria monocytogenes and increased production of H2O2 in response to phorbol myristate acetate. Half-maximal stimulation for macrophage activation (MAF) was observed with 10-20 antiviral U/ml of purified recombinant IFN-gamma. These MAF activities were found to correlate with the antiviral activity dependent on IFN-gamma under several experimental conditions. Both activities were recovered together from supernatants of concanavalin A-stimulated peripheral blood mononuclear cells and in the media of a large number of T cell clones of different specificities. The parallelism between the two activities was also observed upon fractionation of culture media from producing cells and upon treatment of such preparations with low pH and high temperature. Finally, three antibodies with different specificities were found to abrogate the MAF and antiviral activities from lymphocyte culture supernatant. These results indicate that MAF released by stimulated lymphocytes is identical to IFN-gamma.

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Human T lymphocyte clones specific for malaria (Plasmodium falciparum) antigens.

Cited by 34 publications

References 17 publications

Involvement of CD8⁺ T cells in protective immunity against murine blood‐stage infection with Plasmodium yoelii 17XL strain

Involvement of CD8⁺ T cells in protective immunity against murine blood‐stage infection with Plasmodium yoelii 17XL strain

Plasmodium falciparum‐specific human T cell clones: evidence for helper and cytotoxic activities

Identity between human interferon‐γ and “macrophage‐activating factor” produced by human T lymphocytes

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Human T lymphocyte clones specific for malaria (Plasmodium falciparum) antigens.

Cited by 34 publications

References 17 publications

Involvement of CD8+ T cells in protective immunity against murine blood‐stage infection with Plasmodium yoelii 17XL strain

Involvement of CD8+ T cells in protective immunity against murine blood‐stage infection with Plasmodium yoelii 17XL strain

Plasmodium falciparum‐specific human T cell clones: evidence for helper and cytotoxic activities

Identity between human interferon‐γ and “macrophage‐activating factor” produced by human T lymphocytes

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Involvement of CD8⁺ T cells in protective immunity against murine blood‐stage infection with Plasmodium yoelii 17XL strain

Involvement of CD8⁺ T cells in protective immunity against murine blood‐stage infection with Plasmodium yoelii 17XL strain