Human T-Lymphotropic Virus Type 1 p30
            <sup>II</sup>
            Functions as a Transcription Factor and Differentially Modulates CREB-Responsive Promoters

Zhang, Weiqing; Nisbet, John W.; Bartoe, Joshua T.; Ding, Wei; Lairmore, Michael Dale

doi:10.1128/jvi.74.23.11270-11277.2000

Cited by 73 publications

(112 citation statements)

References 33 publications

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“…p30, expressed from a doubly-spliced mRNA, is a multifunctional regulator that differentially modulates viral and/or cellular gene expression at the transcriptional level through association with cellular proteins including p300/CBP and TIP60 (Awasthi et al, 2005;Zhang et al, 2001;Zhang et al, 2000) and post-transcriptionally via binding and retaining tax/rex mRNA in the nucleus (Nicot et al, 2004;Younis et al, 2006;Younis et al, 2004). Although p30 is dispensable in vitro for replication and cellular transformation (Derse et al, 1997;Robek et al, 1998), it is required to promote virus survival and persistence in infected animals (Silverman et al, 2004).…”

Section: Introductionmentioning

confidence: 99%

Detection and quantitation of HTLV-1 and HTLV-2 mRNA species by real-time RT-PCR

Green

2007

Journal of Virological Methods

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SummaryHTLV-1 and HTLV-2 are highly related delta-retroviruses that infect and transform T-lymphocytes, but have distinct pathogenic properties. HTLV replication and survival requires the expression of multiple gene products from an unspliced and a series of highly related alternatively spliced mRNA species. To date, the comparative levels of all known HTLV-1 and HTLV-2 viral mRNAs in different transformed cell lines and at different stages of virus infection have not been assessed. In this study, we compiled a series of oligonucleotide primer pairs and probes to quantify both HTLV-1 and HTLV-2 mRNA species using real-time RT-PCR. The optimized reaction for detection of each mRNA had amplification efficiency greater than 90% with a linear range spanning 25 to 2.5 × 10 7 copies. The R 2 s of all standard curves were greater than 0.97. Quantitation of HTLV mRNAs between different cell lines showed variability (gag/pol ≥ tax/rex > env ≥ accessory proteins), but the overall levels of each mRNA relative to each other within a cell line were similar. These results provide a method to quantify all specific mRNAs from both HTLV-1 and HTLV-2, which can be used to evaluate further viral gene expression and correlate transcript levels to key stages of the virus life cycle and ultimately, pathogenesis.

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Section: Introductionmentioning

confidence: 99%

Detection and quantitation of HTLV-1 and HTLV-2 mRNA species by real-time RT-PCR

Green

2007

Journal of Virological Methods

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“…These proteins are important for in vivo viral infectivity, host cell activation, and regulation of gene transcription (Collins et al, 1998;Ciminale et al, 1999;Trovato et al, 1999;Albrecht et al, 2000Albrecht et al, , 2002Bartoe et al, 2000;D'Agostino et al, 2000;Zhang et al, 2000Zhang et al, , 2001Ding et al, 2002;Franchini et al, 2003;Kim et al, 2003;Michael et al, 2004;Nicot et al, 2004;Younis et al, 2004). The role of these accessory proteins in HTLV-1-mediated transformation has not been …”

Section: Htlv-1 Replication and Mechanisms Of Carcinogenesismentioning

confidence: 99%

Animal models for human T-lymphotropic virus type 1 (HTLV-1) infection and transformation

2005

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Over the past 25 years, animal models of human T-lymphotropic virus type 1 (HTLV-1) infection and transformation have provided critical knowledge about viral and host factors in adult T-cell leukemia/lymphoma (ATL). The virus consistently infects rabbits, some non-human primates, and to a lesser extent rats. In addition to providing fundamental concepts in viral transmission and immune responses against HTLV-1 infection, these models have provided new information about the role of viral proteins in carcinogenesis. Mice and rats, in particular immunodeficient strains, are useful models to assess immunologic parameters mediating tumor outgrowth and therapeutic invention strategies against lymphoma. Genetically altered mice including both transgenic and knockout mice offer important models to test the role of specific viral and host genes in the development of HTLV-1-associated lymphoma. Novel approaches in genetic manipulation of both HTLV-1 and animal models are available to address the complex questions that remain about viral-mediated mechanisms of cell transformation and disease. Current progress in the understanding of the molecular events of HTLV-1 infection and transformation suggests that answers to these questions are approachable using animal models of HTLV-1-associated lymphoma Oncogene (2005) 24, 6005-6015.

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Section: Htlv-1 Orf I: P12 I a Modulator Of T-cell Immune Functionsmentioning

confidence: 99%

“…In fact, recent studies show that, in addition to its post-transcriptional functions, ectopically expressed p30 II differentially affects the basal level of transcription from CREB-responsive elements (CREs) and Tax-responsive elements (TREs) (Zhang et al, 2000. Zhang et al (2000) demonstrated that p30 II interacts with the transcriptional cofactors p300/ CREB-binding protein and colocalizes with p300 in cells. A Gal4 (DNA-binding domain, DBD)-p30 II fusion protein was shown to contain a functional transcriptional activation domain residing between amino acids 62 and 220 of p30 II (Zhang et al, 2000) (Figure 3).…”

Section: Htlv-1 Orf I: P12 I a Modulator Of T-cell Immune Functionsmentioning

confidence: 99%

Human T-cell leukemia/lymphoma virus type 1 nonstructural genes and their functions

et al. 2005

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The human T-cell leukemia/lymphoma virus (HTLV) genome, in addition to the structural Gag and Env proteins and retroviral enzymes, carries a region at its 3 0 end originally designated pX. To date, we know that this region encodes two essential transcriptional and posttranscriptional positive regulators of viral expression, the Tax and Rex proteins, respectively (reviewed elsewhere in this issue). Here, we will review current knowledge of the functions of three additional proteins encoded in the pX region, p12 I , p13 II , and p30 II .

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Human T-Lymphotropic Virus Type 1 p30 ^II Functions as a Transcription Factor and Differentially Modulates CREB-Responsive Promoters

Cited by 73 publications

References 33 publications

Detection and quantitation of HTLV-1 and HTLV-2 mRNA species by real-time RT-PCR

Detection and quantitation of HTLV-1 and HTLV-2 mRNA species by real-time RT-PCR

Animal models for human T-lymphotropic virus type 1 (HTLV-1) infection and transformation

Human T-cell leukemia/lymphoma virus type 1 nonstructural genes and their functions

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Human T-Lymphotropic Virus Type 1 p30 II Functions as a Transcription Factor and Differentially Modulates CREB-Responsive Promoters

Cited by 73 publications

References 33 publications

Detection and quantitation of HTLV-1 and HTLV-2 mRNA species by real-time RT-PCR

Detection and quantitation of HTLV-1 and HTLV-2 mRNA species by real-time RT-PCR

Animal models for human T-lymphotropic virus type 1 (HTLV-1) infection and transformation

Human T-cell leukemia/lymphoma virus type 1 nonstructural genes and their functions

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Human T-Lymphotropic Virus Type 1 p30 ^II Functions as a Transcription Factor and Differentially Modulates CREB-Responsive Promoters