Human tear proteome dataset in response to daily wear of water gradient contact lens using SWATH-MS approach

Cheung, Jimmy Ka-Wai; Bian, Jingfang; Sze, Ying-Hon; So, Yee-Kiu; Chow, Wing-Yee; Woo, Chun; Wong, Tsz-King; Li, King-Kit; Lam, Thomas Chuen

doi:10.1016/j.dib.2021.107120

Cited by 7 publications

(5 citation statements)

References 5 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…During the DIA process, it is independent of the composition of precursor ions for their fragmentation to implement the approach, and thus, this method improves the accuracy and precision of quantitation and has gained much popularity (Zhang et al, 2020a). This technique is widely used in various body fluids, including blood, cerebrospinal fluid, urine, and tears but is less applied in saliva (Li et al, 2020;van der Laan et al, 2020;Cheung et al, 2021;Jia et al, 2021).…”

Section: Introductionmentioning

confidence: 99%

Impact of the Glycemic Level on the Salivary Proteome of Middle-Aged and Elderly People With Type 2 Diabetes Mellitus: An Observational Study

Jia

Zhang

Sun

et al. 2021

Front. Mol. Biosci.

View full text Add to dashboard Cite

Type 2 diabetes mellitus (T2DM) is an increasing global public health concern, but its impact on the salivary proteome is still unclear. To evaluate the effect of glycemic levels in middle-aged and elderly individuals with T2DM on salivary proteomics, we compared the differences by liquid chromatography tandem mass spectrometry (LC–MS/MS). Unstimulated whole saliva samples from 8 T2DM patients with good glycemic control (G group, HbA1c <6.5%) and 16 patients with poor control (P group, HbA1c ≥6.5%) were analyzed by LC–MS/MS in the data-independent acquisition mode (Clinical register number: ChiCTR1900023582.). After functional annotation, cluster analysis and receiver operating characteristic (ROC) curve analysis were carried out to screen and evaluate candidate proteins. A total of 5,721 proteins were quantified, while 40 proteins differed significantly. In the P group, proteins involved in oxidative stress-related processes were upregulated, whereas proteins related to salivary secretion were downregulated. The combination of thioredoxin domain-containing protein 17, zymogen granule protein 16B, and FAM3 metabolism regulating signaling molecule D yielded an area under the curve of 0.917 which showed a robust ability to distinguish the P and G groups. In conclusion, poorly controlled hyperglycemia may affect salivary proteins through various pathways, including oxidative stress and glandular secretion. Furthermore, the differentially expressed proteins, especially the three proteins with the best differentiation, might serve as an anchor point for the further study of hyperglycemia and oral diseases.

show abstract

Section: Introductionmentioning

confidence: 99%

Impact of the Glycemic Level on the Salivary Proteome of Middle-Aged and Elderly People With Type 2 Diabetes Mellitus: An Observational Study

Jia

Zhang

Sun

et al. 2021

Front. Mol. Biosci.

View full text Add to dashboard Cite

show abstract

“…The cells were then trypsinized and harvested (normal glycemic control: n = 3, high glucose: n = 6). The cell pellets were collected after centrifugation at 300× g and treated with S-Trap SDS protein solubilization buffer containing 10% SDS and 100mM triethylammonium bicarbonate (TEAB) [ 73 ]. After sonication on ice for 30 min, the protein concentrations were assessed by a Pierce TM BCA gold protein assay (Thermo Fisher Scientific, Rockford, IL, USA) according to the manufacturer’s guidelines.…”

Section: Methodsmentioning

confidence: 99%

Proteomic Profiling Revealed Mitochondrial Dysfunction in Photoreceptor Cells under Hyperglycemia

Lam

Cheung

Tse

et al. 2022

IJMS

Self Cite

View full text Add to dashboard Cite

Diabetic retinopathy (DR) was identified as a leading cause of blindness and vision impairment in 2020. In addition to vasculopathy, DR has been found to involve retinal neurons, including amacrine cells and retinal ganglion cells. Despite possessing features that are susceptible to diabetic conditions, photoreceptor cells have received relatively little attention with respect to the development of DR. Until recently, studies have suggested that photoreceptors secret proinflammatory molecules and produce reactive oxygen species that contribute to the development of DR. However, the effect of hyperglycemia on photoreceptors and its underlying mechanism remains elusive. In this study, the direct effect of high glucose on photoreceptor cells was investigated using a 661w photoreceptor-like cell line. A data-independent sequential window acquisition of all theoretical mass spectra (SWATH)-based proteomic approach was employed to study changes induced by high glucose in the proteomic profile of the cells. The results indicated that high glucose induced a significant increase in apoptosis and ROS levels in the 661w cells, with mitochondrial dysfunction among the major affected canonical pathways. The involvement of mitochondrial dysfunction was further supported by increased mitochondrial fission and reduced mitochondrial bioenergetics. Collectively, these findings provide a biological basis for a possible role of photoreceptors in the pathogenesis of DR.

show abstract

“…Tear sample preparation for MS and the LC-MS settings were described in our previous study [19,20]. In brief, the strip's top end (beyond the 0 mm mark) was discarded, and the strip with tear fluid was cut into 1 mm interval spaces and placed into a new Eppendorf tube.…”

Section: Tear Protein Extraction and Liquid Chromatography-mass Spect...mentioning

confidence: 99%

Myopia Control Efficacy and Long-Term Safety of a Novel Orthokeratology Lens (MESOK Study)—A Randomized Controlled Clinical Trial Combining Clinical and Tear Proteomics Data

Choi

Cheung

Wong

et al. 2023

JCM

View full text Add to dashboard Cite

Myopia control efficacy and long-term safety of the Breath-O-Correct orthokeratology (OK) lens was evaluated in a 2-year randomized, single vision (SV) spectacle lens-controlled, single-blind clinical trial combining clinical and tear proteomics data. A total of 71 children (43 OK, 9.8 ± 1.3 years; 28 SV, 9.5 ± 1.4 years) completed the 2-year study. Axial length (AL), cycloplegic refraction, clinical safety parameters (best-corrected visual acuity, central cornea thickness, corneal endothelial health, ocular surface disease index), and quantitative tear proteomics were evaluated by masked examiners. Mean 2-year-normalized AL elongations in the OK and SV groups differed significantly (p = 0.03) and were 0.37 ± 0.37 mm and 0.60 ± 0.41 mm, respectively. OK-mediated myopia control efficacy was 37.1%. No significant difference was found in clinical safety parameters of both groups (p > 0.10), except for a thinner central corneal thickness in the OK group (p = 0.01). Proteomics revealed modest OK lens-mediated effects on immune response proteins, including an increased abundance of haptoglobin at 6 and 12 months and a decreased abundance of two proteins (neutrophil defensin 3 and histone 4) at 6 months. The changes were further validated using a high-resolution multiple-reaction monitoring (MRMHR) mass spectrometry. In summary, the Breath-O-Correct OK lens significantly reduced AL elongation in schoolchildren without adverse clinical effects or subclinical inflammatory responses.

show abstract

Human tear proteome dataset in response to daily wear of water gradient contact lens using SWATH-MS approach

Cited by 7 publications

References 5 publications

Impact of the Glycemic Level on the Salivary Proteome of Middle-Aged and Elderly People With Type 2 Diabetes Mellitus: An Observational Study

Impact of the Glycemic Level on the Salivary Proteome of Middle-Aged and Elderly People With Type 2 Diabetes Mellitus: An Observational Study

Proteomic Profiling Revealed Mitochondrial Dysfunction in Photoreceptor Cells under Hyperglycemia

Myopia Control Efficacy and Long-Term Safety of a Novel Orthokeratology Lens (MESOK Study)—A Randomized Controlled Clinical Trial Combining Clinical and Tear Proteomics Data

Contact Info

Product

Resources

About