Human Tumor Cell Lines Demonstrating the Characteristics of Patient Tumors as Useful Models for Anticancer Drug Screening

Roth, Therese M.; Burger, AM; Dengler, W. A.; Willmann, Hildegard; Fiebig, H. H.

doi:10.1159/000425830

Cited by 39 publications

(24 citation statements)

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“…Twenty-four cell lines out of Oncotest's 36 cell line panel were established at Oncotest from patient-derived tumor xenografts passaged subcutaneously in nude mice [30]. The origin of the donor xenografts have been previously described [31].…”

Section: Methodsmentioning

confidence: 99%

Role of Transferrin Receptor and the ABC Transporters ABCB6 and ABCB7 for Resistance and Differentiation of Tumor Cells towards Artesunate

et al. 2007

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The anti-malarial artesunate also exerts profound anti-cancer activity. The susceptibility of tumor cells to artesunate can be enhanced by ferrous iron. The transferrin receptor (TfR) is involved in iron uptake by internalization of transferrin and is over-expressed in rapidly growing tumors. The ATP-binding cassette (ABC) transporters ABCB6 and ABCB7 are also involved in iron homeostasis. To investigate whether these proteins play a role for sensitivity towards artesunate, Oncotest's 36 cell line panel was treated with artesunate or artesunate plus iron(II) glycine sulfate (Ferrosanol®). The majority of cell lines showed increased inhibition rates, for the combination of artesunate plus iron(II) glycine sulfate compared to artesunate alone. However, in 11 out of the 36 cell lines the combination treatment was not superior. Cell lines with high TfR expression significantly correlated with high degrees of modulation indicating that high TfR expressing tumor cells would be more efficiently inhibited by this combination treatment than low TfR expressing ones. Furthermore, we found a significant relationship between cellular response to artesunate and TfR expression in 55 cell lines of the National Cancer Institute (NCI), USA. A significant correlation was also found for ABCB6, but not for ABCB7 in the NCI panel. Artesunate treatment of human CCRF-CEM leukemia and MCF7 breast cancer cells induced ABCB6 expression but repressed ABCB7 expression. Finally, artesunate inhibited proliferation and differentiation of mouse erythroleukemia (MEL) cells. Down-regulation of ABCB6 by antisense oligonucleotides inhibited differentiation of MEL cells indicating that artesunate and ABCB6 may cooperate. In conclusion, our results indicate that ferrous iron improves the activity of artesunate in some but not all tumor cell lines. Several factors involved in iron homeostasis such as TfR and ABCB6 may contribute to this effect.

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Section: Methodsmentioning

confidence: 99%

Role of Transferrin Receptor and the ABC Transporters ABCB6 and ABCB7 for Resistance and Differentiation of Tumor Cells towards Artesunate

et al. 2007

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“…Cell lines derived from the Oncotest collection were established from human tumor xenografts as described by Roth et al (1999). The origin of the donor xenografts was described by Fiebig et al (1992Fiebig et al ( , 1999.…”

Section: Antitumor Activitymentioning

confidence: 99%

Characterization of the anticancer properties of monoglycosidic cardenolides isolated from Nerium oleander and Streptocaulon tomentosum

Rashan

Franke

Khine

et al. 2011

Journal of Ethnopharmacology

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“…These 12 cell lines include generally sensitive cell lines as well as those with well-sdocumented characteristics including resistance to some anti-cancer compounds. 13,14 The data produced allowed a simple SAR analysis of this set of compounds. At the concentration studied (10 lM) 1, 3 and 4 were equipotent as judged by mean growth inhibition of treated cells versus untreated (%T/C).…”

mentioning

confidence: 99%

Separation of anti-angiogenic and cytotoxic activities of borrelidin by modification at the C17 side chain

Wilkinson¹,

Gregory²,

Moss³

et al. 2006

Bioorganic & Medicinal Chemistry Letters

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Human Tumor Cell Lines Demonstrating the Characteristics of Patient Tumors as Useful Models for Anticancer Drug Screening

Cited by 39 publications

References 0 publications

Role of Transferrin Receptor and the ABC Transporters ABCB6 and ABCB7 for Resistance and Differentiation of Tumor Cells towards Artesunate

Role of Transferrin Receptor and the ABC Transporters ABCB6 and ABCB7 for Resistance and Differentiation of Tumor Cells towards Artesunate

Characterization of the anticancer properties of monoglycosidic cardenolides isolated from Nerium oleander and Streptocaulon tomentosum

Separation of anti-angiogenic and cytotoxic activities of borrelidin by modification at the C17 side chain

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