Human umbilical cord‑derived mesenchymal stem cells ameliorate the enteropathy of food allergies in mice

Abstract

Food allergy prevalence has steadily increased worldwide over the past decades and immunotherapeutic treatment strategies are gaining attention. Human umbilical cord-derived mesenchymal stem cells (hUC-MSCs) exhibit similar immune regulatory properties to bone marrow-derived MSCs. hUC-MCSs can be prepared with fewer ethical constraints and are potential candidates for allergic disorder therapies. The current study aimed to investigate potential antiallergic properties of hUC-MSCs in mice with ovalbumin (OVA)-induced food allergy. Administration of hUC-MSCs cells intraperitoneally combined with oral gavage of the culture medium significantly alleviated OVA-induced diarrhea symptoms. Additionally, this treatment significantly decreased IgE levels and the percentage of T helper 2 cells in the blood, which were increased in mice with OVA-induced food allergy. The mRNA levels of the inflammatory cytokines interleukin-4 and tumor necrosis factor-α, and inflammatory cell infiltration in mouse colons were significantly decreased in hUC-MSCs-treated animals compared with mice with OVA-induced food allergy. Goblet cells were detected in colons of allergy-induced mice and their numbers were reduced following treatment with hUC-MSCs. In addition, treatment with hUC-MSCs reestablished the gut flora. The results revealed that hUC-MSCs may have a potential application in food allergy therapy.

hUCMSCs carrying exenatide prevent T1DM by improving intestinal microflora composition and islet tissue damage repair

“…A number of studies have further demonstrated the beneficial effect of hUCMSCs on intestinal microbiota in a variety of diseases. hUCMSCs were found to facilitate the re-establishment of the intestinal microbiota in food allergy induced by ovalbumin (Yan et al 2018 ). Additionally, hUCMSCs were reported to exert protection against experimental colitis through CD5 + B regulatory cells (Chao et al 2016 ).…”

Intranasal administration of Acinetobacter lwoffii in a murine model of asthma induces IL‐6‐mediated protection associated with cecal microbiota changes

“…The AL asthma protection associated with increased Muribaculaceae (previously called S24-7 47 ) and other Bacteroides species is consistent with their critical roles in early life immunological development. [48][49][50] Muribaculaceae have been associated with alleviation of allergic lung inflammation 51 food allergy 52,53 and with type 1 diabetes protection. 54 Our observations pertaining to Parasutterella are consistent with beneficial metabolic roles of these species.…”

Preventive Effects of a Human Hematopoietic Mesenchymal Stem Cell (hHMSC) Therapy in Ovalbumin-Induced Food Allergy

“…The results suggested that the soluble factors secreted by MSCs play a major role in regulating immunologic function in the food allergy model. In the study of human umbilical cord-derived MSCs in the food allergy model, allergic symptoms and IL-4, TNF-α mRNA levels, inflammatory cells, and goblet cells in the colon were decreased by MSCs and MSC-CM [ 39 ]. Similar findings were presented in other allergic disease models.…”

“…MSCs significantly reversed the higher levels of OVA-induced IL-4 and TNF-α mRNA expressed in the OVA. Treatment with MSC-CM by oral gavage partially restored the gut microbiota and goblet cells as well as commensal bacteria in the gut [ 39 ].…”