Human Umbilical Cord Mesenchymal Stem Cells Therapy in Cyclophosphamide-Induced Premature Ovarian Failure Rat Model

Abstract

Premature ovarian failure (POF) is one of the most common causes of infertility in women. In our present study, we established cyclophosphamide- (CTX-) induced POF rat model and elucidated its effect on ovarian function. We detected the serum estrogen, follicle stimulating hormone, and anti-Müllerian hormone of mice models by ELISA and evaluated their folliculogenesis by histopathology examination. Our study revealed that CTX administration could severely disturb hormone secretion and influence folliculogenesis in rat. This study also detected ovarian cells apoptosis by deoxy-UTP-digoxigenin nick end labeling (TUNEL) and demonstrated marked ovarian cells apoptosis in rat models following CTX administration. In order to explore the potential of human umbilical cord mesenchymal stem cells (UCMSCs) in POF treatment, the above indexes were used to evaluate ovarian function. We found that human UCMSCs transplantation recovered disturbed hormone secretion and folliculogenesis in POF rat, in addition to reduced ovarian cell apoptosis. We also tracked transplanted UCMSCs in ovaries by fluorescence in situ hybridization (FISH). The results manifested that the transplanted human UCMSCs could reside in ovarian tissues and could survive for a comparatively long time without obvious proliferation. Our present study provides new insights into the great clinical potential of human UCMSCs in POF treatment.

The effects of human menstrual blood stem cells‐derived granulosa cells on ovarian follicle formation in a rat model of premature ovarian failure

“…These results are in line with the results of other previous studies (Liu et al, 2014;Sheikhansari, Aghebati-Maleki, Nouri, Jadidi-Niaragh, & Yousefi, 2018;Song et al, 2016;Sun et al, 2013;Woods et al, 2013;Xiao et al, 2016).…”

“…In recent years, the use of MSCs as cell therapy for the treatment of POF has been investigated in many studies. MSCs from various sources have been used for this purpose, and it has been found that these cells have different abilities in improving ovarian function (Fu, He, Xie, & Liu, ; Song et al, ; Sun et al, ; Xiao et al, ). In a previous study, a successful treatment of POF mice using MSC transplantation was reported (Lai et al, ; Liu et al, ; Wang et al, ).…”

The effects of human umbilical cord mesenchymal stem cell transplantation on female fertility preservation in mice

“…This experiment reported that the ratio of tubal factor infertility in women over 35 years old is significantly higher than it is in women under 30 years old. Second, chemotherapy damages the function of the ovary mainly because it can induce the apoptosis of granulosa cells, it can damage the ovarian microenvironment, and it can injure growing follicles (Roness, et al 2014;Song, et al 2016). Chemotherapy also damaged the morphology of fallopian tubes, as shown in our study.…”

Can mesenchymal stem cells improve spermatogonial stem cell transplantation efficiency?

“…Recently, Song and colleagues transplanted human UC‐MSCs derived from a male baby and successfully tracked them in ovaries by fluorescence in situ hybridization using the Y chromosome as a marker. UC‐MSCs restored the hormone balance, stimulated folliculogenesis, and reduced ovarian cell apoptosis (Song et al ., ). MSC‐derived oogenesis did not result in fully functional follicles, but bone marrow infusions in mice helped to postpone age‐related reproductive failures and also improved offspring survival (Selesniemi et al ., ).…”