Human umbilical cord mesenchymal stem cell-derived and dermal fibroblast-derived extracellular vesicles protect dermal fibroblasts from ultraviolet radiation-induced photoaging in vitro

Deng, Mingwu; Yu, Tingting; Liu, Dong; Wang, Xiangsheng; Zhou, Guangdong; Liu, Wei; Cao, Yilin; Xia, Wanyao; Li, Wei; Zhang, Wen Jie

doi:10.1039/c9pp00421a

Cited by 36 publications

(40 citation statements)

References 41 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…58,59 Studies using Exo ucMSC and Exo BM-MSC treatment reported increased proliferation of HDF only at 72 hours post-treatment, but the effects at earlier time points were not investigated. 30,58 In this work, murine 3T3 fibroblasts treated with Exo ucMSC showed significantly accelerated migration only at 48 hours, while increased proliferation was observed at 48 and 72 hours post-treatment (Figures 3 and 4). This work is also the first to investigate the effect of Exo ucMSC on migration and proliferation of murine-derived fibroblasts.…”

Section: F I G U R Ementioning

confidence: 52%

“…Other studies using Exo BM‐MSC and exosomes derived from AD‐MSC (Exo AD‐MSC ) reported accelerated human fibroblast migration as early as 16 and 18 hours post‐treatment, respectively 58,59 . Studies using Exo ucMSC and Exo BM‐MSC treatment reported increased proliferation of HDF only at 72 hours post‐treatment, but the effects at earlier time points were not investigated 30,58 . In this work, murine 3T3 fibroblasts treated with Exo ucMSC showed significantly accelerated migration only at 48 hours, while increased proliferation was observed at 48 and 72 hours post‐treatment (Figures 3 and 4).…”

Section: Discussionmentioning

confidence: 68%

“…Exo ucMSC were also reported to inhibit undesired immune responses such as in the context of organ transplantation and burn‐induced inflammation 25,26 . Among the reported regenerative effects of Exo ucMSC include improved recovery in cutaneous wound healing, 27,28 endometrial injury, 29 and UVB‐induced photoaging 30 …”

Section: Introductionmentioning

confidence: 99%

“…25,26 Among the reported regenerative effects of Exo ucMSC include improved recovery in cutaneous wound healing, 27,28 endometrial injury, 29 and UVB-induced photoaging. 30 Unfortunately, these studies used FBS in their ucMSC culture, which harbors risks for clinical translation of stem cell-based therapy such as harmful xenogeneic immune responses, either by internalization and subsequent antigen presentation, or by attachment to cell surface, and therefore, becomes antigenic substrates following transplantation. 31,32 Other concerns of FBS contamination in administering cells for therapeutic applications include risks of viral and prion transmission, 33 as well as zoonotic infections from metabolic incorporation of xenogeneic sialic acid Neu5Gc.…”

mentioning

confidence: 99%

See 3 more Smart Citations

Defined serum‐free three‐dimensional culture of umbilical cord‐derived mesenchymal stem cells yields exosomes that promote fibroblast proliferation and migration in vitro

et al. 2020

View full text Add to dashboard Cite

Stem cell‐derived exosomes are emerging as novel and clinically relevant cell‐free therapeutics for regenerative therapy. This work focused on investigating the stimulation of fibroblasts by exosomes derived from umbilical cord‐derived mesenchymal stem cells (ucMSC) in a defined serum‐free three‐dimensional (3D) culture. 3D culture of ucMSC was carried out in medium supplemented with KnockOut serum replacement (KO‐medium) using the Aggrewell system. ucMSC in KO‐medium formed spheroids with maintained size and integrity throughout culture. This enabled the isolation of vesicles from ucMSC spheroids in KO‐medium with sizes that fall within the exosomal size range and were positive for the expression of canonical exosomal markers CD63, CD9, CD81, Alix, and TSG101. The ucMSC‐derived exosomes (ExoucMSC) were shown to significantly increase the migration and proliferation of murine fibroblasts in vitro. To conclude, 3D culture of ucMSC in defined serum‐free KO‐medium formed viable spheroids which enabled the isolation of ExoucMSC with the potential of accelerating wound healing.

show abstract

Section: F I G U R Ementioning

confidence: 52%

Section: Discussionmentioning

confidence: 68%

Section: Introductionmentioning

confidence: 99%

mentioning

confidence: 99%

See 2 more Smart Citations

Defined serum‐free three‐dimensional culture of umbilical cord‐derived mesenchymal stem cells yields exosomes that promote fibroblast proliferation and migration in vitro

et al. 2020

View full text Add to dashboard Cite

show abstract

“…Similarly, fibroblast-EVs could shuttle miR-23a-3p to accelerate scratch closure of epidermal keratinocytes in vitro 136 . Moreover, fibroblast-EVs significantly inhibited the production of ROS and cell death in fibroblasts induced by ultraviolet B radiation, thus playing a critical role in skin homeostasis during photoaging 137 .…”

Section: Characteristics and Functions Of Evs From Cells Associated Wmentioning

confidence: 97%

Extracellular vesicles in Inflammatory Skin Disorders: from Pathophysiology to Treatment

Shao¹,

Fang²,

Li³

et al. 2020

Theranostics

View full text Add to dashboard Cite

Extracellular vesicles (EVs), naturally secreted by almost all known cell types into extracellular space, can transfer their bioactive cargos of nucleic acids and proteins to recipient cells, mediating cell-cell communication. Thus, they participate in many pathogenic processes including immune regulation, cell proliferation and differentiation, cell death, angiogenesis, among others. Cumulative evidence has shown the important regulatory effects of EVs on the initiation and progression of inflammation, autoimmunity, and cancer. In dermatology, recent studies indicate that EVs play key immunomodulatory roles in inflammatory skin disorders, including psoriasis, atopic dermatitis, lichen planus, bullous pemphigoid, systemic lupus erythematosus, and wound healing. Importantly, EVs can be used as biomarkers of pathophysiological states and/or therapeutic agents, both as carriers of drugs or even as a drug by themselves. In this review, we will summarize current research advances of EVs from different cells and their implications in inflammatory skin disorders, and further discuss their future applications, updated techniques, and challenges in clinical translational medicine.

show abstract

Roles of extracellular vesicles in the aging microenvironment and age‐related diseases

Yin

Chen

Wang

et al. 2021

J of Extracellular Vesicle

103

View full text Add to dashboard Cite

Cellular senescence is a persistently hypoproliferative state with diverse stressors in a specific aging microenvironment. Senescent cells have a double-edged sword effect: they can be physiologically beneficial for tissue repair, organ growth, and body homeostasis, and they can be pathologically harmful in age-related diseases. Among the hallmarks of senescence, the SASP, especially SASP-related extracellular vesicle (EV) signalling, plays the leading role in aging transmission via paracrine and endocrine mechanisms. EVs are successful in intercellular and interorgan communication in the aging microenvironment and age-related diseases. They have detrimental effects on downstream targets at the levels of immunity, inflammation, gene expression, and metabolism. Furthermore, EVs obtained from different donors are also promising materials and tools for antiaging treatments and are used for regeneration and rejuvenation in cell-free systems. Here, we describe the characteristics of cellular senescence and the aging microenvironment, concentrating on the production and function of EVs in age-related diseases, and provide new ideas for antiaging therapy with EVs.

show abstract

Human umbilical cord mesenchymal stem cell-derived and dermal fibroblast-derived extracellular vesicles protect dermal fibroblasts from ultraviolet radiation-induced photoaging in vitro

Abstract: In the current study, we proved that MSC-EVs can effectively prevent UVB-induced cell damage via the reduction of intracellular ROS. We believe that this study can provide a new strategy for the prevention of skin photoaging.

Cited by 36 publications

References 41 publications

Defined serum‐free three‐dimensional culture of umbilical cord‐derived mesenchymal stem cells yields exosomes that promote fibroblast proliferation and migration in vitro

Defined serum‐free three‐dimensional culture of umbilical cord‐derived mesenchymal stem cells yields exosomes that promote fibroblast proliferation and migration in vitro

Extracellular vesicles in Inflammatory Skin Disorders: from Pathophysiology to Treatment

Roles of extracellular vesicles in the aging microenvironment and age‐related diseases

Contact Info

Product

Resources

About