Human umbilical cord mesenchymal stem cells-derived exosomes for treating traumatic pancreatitis in rats

Han, Lei; Zhao, Zhirong; Chen, Xingyun; Yang, Kè; Tan, Zhenghuai; Huang, Zhu; Lichen, Zhou; Dai, Ruiwu

doi:10.1186/s13287-022-02893-1

Cited by 10 publications

(12 citation statements)

References 29 publications

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“…Approximately 1 mL normal saline was injected via the tail veins of rats. In the TP group, the modelling approach is presented in a previous study 4 . In the TP+ ucMSC‐Ex + rapamycin group, a TP model was established for this group.…”

Section: Methodsmentioning

confidence: 99%

Still water run deep: Therapeutic TP effect of ucMSC‐Ex via regulating mTOR to enhance autophagy

Zhao,

Han,

Xin

et al. 2024

J Cellular Molecular Medi

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Our previous study confirmed that umbilical cord mesenchymal stem cells‐exosomes (ucMSC‐Ex) inhibit apoptosis of pancreatic acinar cells to exert protective effects. However, the relationship between apoptosis and autophagy in traumatic pancreatitis (TP) has rarely been reported. We dissected the transcriptomics after pancreatic trauma and ucMSC‐Ex therapy by high‐throughput sequencing. Additionally, we used rapamycin and MHY1485 to regulate mTOR. HE, inflammatory factors and pancreatic enzymatic assays were used to comprehensively determine the local versus systemic injury level, fluorescence staining and electron microscopy were used to detect the effect of autophagy, and observe the expression levels of autophagy‐related markers at the gene and protein levels. High‐throughput sequencing identified that autophagy played a crucial role in the pathophysiological process of TP and ucMSC‐Ex therapy. The results of electron microscopy, immunofluorescence staining, polymerase chain reaction and western blot suggested that therapeutic effect of ucMSC‐Ex was mediated by activation of autophagy in pancreatic acinar cells through inhibition of mTOR. ucMSC‐Ex can attenuate pancreas injury by inhibiting mTOR to regulate acinar cell autophagy after TP. Future studies will build on the comprehensive sequencing of RNA carried by ucMSC‐Ex to predict and verify specific non‐coding RNA.

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Section: Methodsmentioning

confidence: 99%

Still water run deep: Therapeutic TP effect of ucMSC‐Ex via regulating mTOR to enhance autophagy

Zhao,

Han,

Xin

et al. 2024

J Cellular Molecular Medi

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“…Human umbilical cord mesenchymal stem cells (hucMSC) have been frequently recognized for their capacity for self-renewal and multilineage differentiation, particularly for the bioactive chemicals they contain for tissue repair and the management of inflammation [ 177 , 178 ]. Han et al discovered that hucMSC-derived exosomes showed a remarkable tissue regeneration potential in rats suffering from traumatic pancreatitis (trauma-induced non-infectious AP) [ 179 ]. In particular, hucMSC-Evs injected intravenously could colonize injured pancreatic tissue and inhibit inflammatory response and apoptosis of acinar cells, promoting damaged tissue repair [ 179 ].…”

Section: The Therapeutic Potential Of Exosomes In Ap and Associated Alimentioning

confidence: 99%

“…Han et al discovered that hucMSC-derived exosomes showed a remarkable tissue regeneration potential in rats suffering from traumatic pancreatitis (trauma-induced non-infectious AP) [ 179 ]. In particular, hucMSC-Evs injected intravenously could colonize injured pancreatic tissue and inhibit inflammatory response and apoptosis of acinar cells, promoting damaged tissue repair [ 179 ]. A less frequent consequence of AP is myocardial injury, which is challenging to treat and has a high death rate [ 180 ].…”

Section: The Therapeutic Potential Of Exosomes In Ap and Associated Alimentioning

confidence: 99%

Fighting Fire with Fire: Exosomes and Acute Pancreatitis-Associated Acute Lung Injury

et al. 2022

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Acute pancreatitis (AP) is a prevalent clinical condition of the digestive system, with a growing frequency each year. Approximately 20% of patients suffer from severe acute pancreatitis (SAP) with local consequences and multi-organ failure, putting a significant strain on patients’ health insurance. According to reports, the lungs are particularly susceptible to SAP. Acute respiratory distress syndrome, a severe type of acute lung injury (ALI), is the primary cause of mortality among AP patients. Controlling the mortality associated with SAP requires an understanding of the etiology of AP-associated ALI, the discovery of biomarkers for the early detection of ALI, and the identification of potentially effective drug treatments. Exosomes are a class of extracellular vesicles with a diameter of 30–150 nm that are actively released into tissue fluids to mediate biological functions. Exosomes are laden with bioactive cargo, such as lipids, proteins, DNA, and RNA. During the initial stages of AP, acinar cell-derived exosomes suppress forkhead box protein O1 expression, resulting in M1 macrophage polarization. Similarly, macrophage-derived exosomes activate inflammatory pathways within endothelium or epithelial cells, promoting an inflammatory cascade response. On the other hand, a part of exosome cargo performs tissue repair and anti-inflammatory actions and inhibits the cytokine storm during AP. Other reviews have detailed the function of exosomes in the development of AP, chronic pancreatitis, and autoimmune pancreatitis. The discoveries involving exosomes at the intersection of AP and acute lung injury (ALI) are reviewed here. Furthermore, we discuss the therapeutic potential of exosomes in AP and associated ALI. With the continuous improvement of technological tools, the research on exosomes has gradually shifted from basic to clinical applications. Several exosome-specific non-coding RNAs and proteins can be used as novel molecular markers to assist in the diagnosis and prognosis of AP and associated ALI.

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“…At present, with the gradual progress of research, exosomes have been paid more and more attention. For example, umbilical cord mesenchymal stem cell-derived exosomes (ucMSC-Exs) were validated in our previous study to promote pancreatic tissue repair in traumatic pancreatitis injury by inhibiting pancreatic acinar cell apoptosis and controlling the systemic inflammatory response [ 13 ]. Kong et al [ 14 ] investigated the effect of cancer cell-derived exosomes linc00313 on M2 macrophage differentiation in non-small cell lung cancer (NSCLC).…”

Section: Introductionmentioning

confidence: 99%

Removing the stumbling block of exosome applications in clinical and translational medicine: expand production and improve accuracy

Zhao

et al. 2023

Stem Cell Res Ther

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Although the clinical application and transformation of exosomes are still in the exploration stage, the prospects are promising and have a profound impact on the future transformation medicine of exosomes. However, due to the limitation of production and poor targeting ability of exosomes, the extensive and rich biological functions of exosomes are restricted, and the potential of clinical transformation is limited. The current research is committed to solving the above problems and expanding the clinical application value, but it lacks an extensive, multi-angle, and comprehensive systematic summary and prospect. Therefore, we reviewed the current optimization strategies of exosomes in medical applications, including the exogenous treatment of parent cells and the improvement of extraction methods, and compared their advantages and disadvantages. Subsequently, the targeting ability was improved by carrying drugs and engineering the structure of exosomes to solve the problem of poor targeting ability in clinical transformation. In addition, we discussed other problems that may exist in the application of exosomes. Although the clinical application and transformation of exosomes are still in the exploratory stage, the prospects are promising and have a profound impact on drug delivery, clinical diagnosis and treatment, and regenerative medicine.

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Human umbilical cord mesenchymal stem cells-derived exosomes for treating traumatic pancreatitis in rats

Cited by 10 publications

References 29 publications

Still water run deep: Therapeutic TP effect of ucMSC‐Ex via regulating mTOR to enhance autophagy

Still water run deep: Therapeutic TP effect of ucMSC‐Ex via regulating mTOR to enhance autophagy

Fighting Fire with Fire: Exosomes and Acute Pancreatitis-Associated Acute Lung Injury

Removing the stumbling block of exosome applications in clinical and translational medicine: expand production and improve accuracy

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