Human urine-derived stem cells in combination with polycaprolactone/gelatin nanofibrous membranes enhance wound healing by promoting angiogenesis

Abstract

BackgroundDespite advancements in wound healing techniques and devices, new treatments are needed to improve therapeutic outcomes. This study aimed to evaluate the potential use of a new biomaterial engineered from human urine-derived stem cells (USCs) and polycaprolactone/gelatin (PCL/GT) for wound healing.MethodsUSCs were isolated from healthy individuals. To fabricate PCL/GT composite meshes, twin-nozzle electrospinning were used to spin the PCL and gelatin solutions in normal organic solvents. The morphologies and hydrophilicity properties of PCL/GT membranes were investigated. After USCs were seeded onto a PCL/GT, cell adhesion, morphology, proliferation, and cytotoxicity were examined. Then, USCs were seeded on a PCL/GT blend nanofibrous membrane and transplanted into rabbit full-thickness skin defects for wound repair. Finally, the effect of USCs condition medium on proliferation, migration, and tube formation of human umbilical vein endothelial cells (HUVECs) were performed in vitro.ResultsUSCs were successfully isolated from urine samples and expressed specific mesenchymal stem cells markers and could differentiate into osteoblasts, adipocytes, and chondrocytes. PCL/GT membrane has suitable mechanical properties similar with skin tissue and has good biocompatibility. USCs-PCL/GT significantly enhanced the healing of full-thickness skin wounds in rabbits compared to wounds treated with PCL/GT membrane alone or untreated wounds. USCs-PCL/GT-treated wounds closed much faster, with increased re-epithelialization, collagen formation, and angiogenesis. Moreover, USCs could secrete VEGF and TGF-β1, and USC-conditioned medium enhanced the migration, proliferation, and tube formation of endothelial cells.ConclusionUSCs in combination with PCL/GT significantly prompted the healing of full-thickness skin wounds in rabbits. USCs based therapy provides a novel strategy for accelerating wound closure and promoting angiogenesis.

The Effect of Estradiol on the Growth Plate Chondrocytes of Limb and Spine from Postnatal Mice in vitro: The Role of Estrogen-Receptor and Estradiol Concentration

“…Meanwhile, the expression of type X collagen is the typical features of chondrocyte hypertrophy 20-24. These observed indicators were commensurate with previously reported results, and were adopted to measure the chondrogenesis process 25-29.…”

Extracellular Vesicles Secreted by Human Urine-Derived Stem Cells Promote Ischemia Repair in a Mouse Model of Hind-Limb Ischemia

“…In previous urological studies, USCs were used to construct tissue-engineered conduits for urinary diversion [15] and to ameliorate erectile dysfunction [16,17]. Moreover, we found that USCs combined with bioactive materials accelerated skin-wound healing in rabbits [18] and that USCs can survive in and migrate to other parenchymal areas and differentiate into neuron-like cells in the rat brain [13].…”

“…USCs were isolated from the urine of healthy adult donors, according to the methods described in our previous studies [13,18]. The Ethics Committee of Shanghai Sixth People's Hospital Affiliated with Shanghai Jiaotong University approved the study, and written informed consent was obtained from all the volunteers upon their enrollment in the study.…”

Core-Sheath Nanofibers as Drug Delivery System for Thermoresponsive Controlled Release

“…[1][2][3] Coaxial electrospinning, comparing to simple single and blended spinning, is used to prepare core-sheath structured nanofibers with outstanding properties and multifunctionality for additional functional applications, [4][5] such as biotechnology, textiles, membranes, composites. [6][7][8][9] Stimuli-responsive polymers have been widely used in intelligent or smart materials with a great promise in drug delivery systems, sensors, and biomaterials, [10][11][12][13][14][15] due to these polymers can respond to changes in temperature, pH, light, or ionic strength. For example, poly(N-isopropylacrylamide) (PNIPAAm) is a typical temperature-sensitive polymer.…”