Human viral nucleic acids concentrations in wastewater solids from Central and Coastal California USA

Boehm, Alexandria B.; Wolfe, Marlene K.; Wigginton, Krista; Bidwell, Amanda; White, Bradley J.; Hughes, Bridgette; Duong, Dorothea; Chan-Herur, Vikram; Bischel, Heather N.; Naughton, Colleen C.

doi:10.1038/s41597-023-02297-7

Cited by 41 publications

(56 citation statements)

References 33 publications

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“…Wastewater data are publicly available (). Data collected between 7/1/22 and 12/31/22, and expanded methods, are publicly available through a Data Descriptor …”

Section: Methodsmentioning

confidence: 99%

More than a Tripledemic: Influenza A Virus, Respiratory Syncytial Virus, SARS-CoV-2, and Human Metapneumovirus in Wastewater during Winter 2022–2023

Boehm

Wolfe

White³

et al. 2023

Environ. Sci. Technol. Lett.

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Wastewater monitoring can provide insights into respiratory disease occurrence in communities that contribute to the wastewater system. Using daily measurements of RNA of influenza A (IAV), respiratory syncytial virus (RSV), and human metapneumovirus (HMPV), as well as SARS-CoV-2 in wastewater solids from eight publicly owned treatment works in the Greater San Francisco Bay Area of California between July 2022 and early July 2023, we identify a “tripledemic” when concentrations of IAV, RSV, and SARS-CoV-2 peaked at approximately the same time. HMPV was also widely circulating. We designed novel hydrolysis probe RT-PCR assays for different IAV subtype markers to discern that the dominant circulating IAV subtype was H3N2. We show that wastewater data can be used to identify the onset and offset of wastewater disease occurrence events. This information can provide insight into disease epidemiology and timely, localized information to inform hospital staffing and clinical decision making to respond to circulating viruses. Whereas RSV and IAV wastewater events were mostly regionally coherent, HMPV events displayed localized occurrence patterns.

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“…Wastewater data are publicly available (). Data collected between 7/1/22 and 12/31/22, and expanded methods, are publicly available through a Data Descriptor …”

Section: Methodsmentioning

confidence: 99%

More than a Tripledemic: Influenza A Virus, Respiratory Syncytial Virus, SARS-CoV-2, and Human Metapneumovirus in Wastewater during Winter 2022–2023

Boehm

Wolfe

White³

et al. 2023

Environ. Sci. Technol. Lett.

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“…A 300 μL portion of the suspension entered into the nucleic-acid extraction process, and 50 μL of nucleic acids is retrieved after the inhibitor removal kit. The preanalytical methods are also provided on protocols.io …”

Section: Methodsmentioning

confidence: 99%

“…This concentration of solids in buffer has been shown to alleviate inhibition in downstream RT-PCR. 34 A separate aliquot of dewatered solids was dried in an oven to determine the dry weight. RNA was extracted from 6 replicate aliquots of dewatered settled solids suspended in the DNA/RNA shield, and then it was subsequently processed through an inhibitor removal kit.…”

Section: ■ Methods and Materialsmentioning

confidence: 99%

Wastewater Detection of Emerging Arbovirus Infections: Case Study of Dengue in the United States

Wolfe,

Paulos,

Zulli

et al. 2023

Environ. Sci. Technol. Lett.

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Climate change and urbanization are increasing the distribution of insect vectors of infectious diseases. Dengue virus is an arbovirus that causes nearly 100 million symptomatic infections per year and is endemic in 124 countries, and the range of its mosquito vectors continues to increase. Surveillance of dengue virus infections is complicated by the fact that infections can be asymptomatic, and symptoms may not be readily recognizable to clinicians. Here we show that wastewater monitoring can be used to detect dengue virus RNA to yield information about the circulation of dengue infections in a community. We collected three samples of wastewater solids per week from three different wastewater treatment plants in Miami-Dade County, Florida, where dengue infections were locally acquired. Using molecular methods, we tested wastewater solids for RNA from the 4 dengue virus serotypes and consistently detected dengue virus 3 RNA at all three wastewater plants and did not detect the other 3 serotypes. According to publicly available data on dengue infections, a vast majority of infections were caused by serotype 3. Wastewater detection of dengue virus RNA is possible with as few as 4.23 laboratory confirmed dengue cases per 1 million people based on publicly available infection data.

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“…The DNA/RNA shield was spiked with bovine coronavirus (BCoV) vaccine as a RNA recovery control. This concentration of solids in buffer has been shown to alleviate inhibition in downstream RT-PCR 34 . A separate aliquot of dewatered solids was dried in an oven to determine dry weight.…”

Section: Methodsmentioning

confidence: 99%

“…RNA was extracted from 6 replicate aliquots of dewatered settled solids suspended in the DNA/RNA Shield, and then it was subsequently processed through an inhibitor removal kit (see SI). The pre-analytical methods are also provided on protocols.io 34 .…”

Section: Methodsmentioning

confidence: 99%

Wastewater detection of emerging arbovirus infections: Case study of Dengue in the United States

Wolfe,

Paulos,

Zulli

et al. 2023

Preprint

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Climate change and urbanization are increasing the distribution of insect vectors of infectious diseases. Dengue virus is an arbovirus that causes nearly 100 million symptomatic infections per year and is endemic in 124 countries, and the range of its mosquito vectors continues to increase. Surveillance of dengue virus infections is complicated by the fact that infections can be asymptomatic and symptoms may not be readily recognizable to clinicians. Here we show that wastewater monitoring can be used to detect dengue virus RNA to yield information about circulation of dengue infections in a community. We collected three samples of wastewater solids per week from three different wastewater treatment plants in Miami-Dade County, Florida where dengue infections have been locally acquired. Using molecular methods, we tested wastewater solids for RNA from the 4 dengue virus serotypes and consistently detected dengue virus 3 RNA at all three wastewater plants, and did not detect the other 3 serotypes. According to publicly available data on dengue infections, the vast majority of infections were caused by serotype 3. Wastewater detection of dengue virus RNA is possible with as few as 4.23 laboratory confirmed dengue cases per 1 million people, based on publicly available infection data.SynopsisDengue virus RNA was detected in wastewater solids in a location with local- and travel-associated dengue infections.

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Human viral nucleic acids concentrations in wastewater solids from Central and Coastal California USA

Cited by 41 publications

References 33 publications

More than a Tripledemic: Influenza A Virus, Respiratory Syncytial Virus, SARS-CoV-2, and Human Metapneumovirus in Wastewater during Winter 2022–2023

More than a Tripledemic: Influenza A Virus, Respiratory Syncytial Virus, SARS-CoV-2, and Human Metapneumovirus in Wastewater during Winter 2022–2023

Wastewater Detection of Emerging Arbovirus Infections: Case Study of Dengue in the United States

Wastewater detection of emerging arbovirus infections: Case study of Dengue in the United States

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