Humic substance extraction from leonardite, lignite Mae Mho Mine by base-acid treatment process

Abstract

Humic substance (HS) is very important for soil agriculture that affects physical and chemical properties and improves soil fertility. Humic substances are complex mixtures of polydispersed materials formed by biochemical and chemical reactions during the decay and transformation of plant and microbial remains. Humic substances were extracted from leonardite of lignite Mae Mho Mine in Lampang province by using base-acid treatment and centrifugation. This research studied the effect of time and temperature in base treatment process and the effect of soils particle size. The humic substance which contain humin, humic acid (HAs) and fulvic acid (FAs) were extracted by base-acid treatment. Firstly, soil sample were stirred in base solution and precipitated by centrifugation. The humin precipitate that the fraction is not soluble in water at any pH value was dried at 90 o C. Then, the soluble(HAs and FAs) were pH adjusted to 2 by using 3M HCl and stirred in room temperature The humic fraction precipitate were separated by centrifugation and was dried at 90 o C. The soluble was pH adjusted to 4-5 and settled for 24 hr. The fulvic acid precipitate was dried at 90 o C. Humic substances were studied for element analysis and chemical structure by FT-IR and CHNO. The yields of humic acid were increased at the increase of soils particle size and base reaction time.

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“…İmamoğlu (2019) suggested leonardite application in beans. Jomhataikool et al (2019) determined that humic acid and leonardite increased leaf and root growth in rice.…”

The effect of humic acid on biological properties of soil and upland rice plants in entisol Coastal Bengkulu City

“…On the other hand, the Inpago 10 variety consistently had the highest yield per plot, both with and without humic acid (Table 6). Humic acid has the ability as a ligand that is able to bind nitrogen in the form of NO3or NH4 + forming complexes, which can temporarily store nutrients in the soil and release them when plants need them, thereby merahucing the occurrence of evaporation of nitrogen in the soil [17]. Based on the results of the interaction analysis of humic acid and upland rice varieties on yields per plot showed significant differences.…”

Soil stabilisation for laying road foundations: Way forward with organic soil treatment

“…This portion of non-living organic matter can also be divided into humified and non-humified organic matter, depending on its degree of humification [14]. The humic substances consist of fulvic acid, humic acid and humin and are categorised as humified organic matter [15]. In comparison, nonhumic substances include carbohydrates, amino acids, proteins, peptides, lipids, waxes, resins, organic acids, alkanes, organic bases, and lignin [16].…”