Hyaluronan and hyaluronectin in the extracellular matrix of human brain tumour stroma

Delpech, Bertrand; Maingonnat, Catherine; Girard, Nicole; Chauzy, Claude; Olivier, Annie; Maunoury, R; Tayot, J; Creissard, P

doi:10.1016/s0959-8049(05)80214-x

Cited by 163 publications

(124 citation statements)

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“…HA is associated with a wide variety of malignant tumors and has been implicated in cancer progression [3][4][5][6][7][8][9]. However, the importance of HA in osteosarcoma has not yet been investigated in terms of basic or clinical relevance.…”

Section: Discussionmentioning

confidence: 99%

“…Furthermore, HA binding proteins control these cellular behaviors through interactions with HA and assembly of pericellular matrices [2]. Increased HA levels are also observed in malignant tumors, which include gastric cancer, colorectal cancer, breast cancer, glioma, lung carcinoma and ovarian cancer [3][4][5][6][7][8][9]. Studies in vitro have demonstrated that HA levels correlate with the invasive and metastatic capacity of tumor cells [10].…”

Section: Introductionmentioning

confidence: 99%

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Antisense inhibition of hyaluronan synthase-2 in human osteosarcoma cells inhibits hyaluronan retention and tumorigenicity

Nishida

Knudson

et al. 2005

Experimental Cell Research

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Osteosarcoma is a common malignant bone tumor associated with childhood and adolescence. The results of numerous studies have suggested that hyaluronan plays an important role in regulating the aggressive behavior of various types of cancer cells. However, no studies have addressed hyaluronan with respect to osteosarcomas. In this investigation, the mRNA expression copy number of three mammalian hyaluronan synthases (HAS) was determined using competitive RT-PCR in the osteoblastic osteosarcoma cell line, MG-63. MG-63 are highly malignant osteosarcoma cells with an abundant hyaluronan-rich matrix. The results demonstrated that HAS-2 is the predominant HAS in MG-63. Accumulation of intracellular hyaluronan increased in association with the proliferative phase of these cells. The selective inhibition of HAS-2 mRNA in MG-63 cells by antisense phosphorothioate oligonucleotides resulted in reduced hyaluronan accumulation by these cells. As expected, the reduction in hyaluronan disrupted the assembly of cell-associated matrices. However, of most interest, coincident with the reduction in hyaluronan, there was a substantial decrease in cell proliferation, a decrease in cell motility and a decrease in cell invasiveness. These data suggest that hyaluronan synthesized by HAS-2 in MG-63 plays a crucial role in osteosarcoma cell proliferation, motility and invasion.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Antisense inhibition of hyaluronan synthase-2 in human osteosarcoma cells inhibits hyaluronan retention and tumorigenicity

Nishida

Knudson

et al. 2005

Experimental Cell Research

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“…Moreover, in breast cancer both HYA and HN have been found to be more concentrated in invasive areas than in central tumour areas; in both sites HYA concentrations were higher than in normal tissue [11]. HYA concentrations in gliomas was shown to be closer to that of foetal brain than to that of adult brain [12]. Even more striking, however, was the observation that HN was relatively lower in malignant glioblastomas than in low grade astrocytomas, so that the HN/HYA ratio was consequently much lower in the malignant tumours.…”

Section: Some General Considerationsmentioning

confidence: 91%

“…In conflict with this finding, it must be noted that a high molecular mass component in the size range of versican (i.e. 450 kDa) has never been detected with anti-HN antibodies, either in extracts from breast cancer [11] or from gliomas [12] or in cell cultures of fibroblasts or monocytes [8,13]. Recently, however, high molecular mass molecules were detected with anti-HN antibodies in new born rat oligodendrocyte cultures.…”

Section: Macromolecular and Cellular Interactionsmentioning

confidence: 99%

Hyaluronan: fundamental principles and applications in cancer

Delpech

Girard

Bertrand

et al. 1997

Journal of Internal Medicine

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146

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“…In the ECM, the action of HAase produces HA oligosaccharides which favour the production of new blood-vessels facilitating the development of cancer tumours. In addition to many polysaccharides, a lot of proteins are components of the ECM [13,14].…”

Section: Introductionmentioning

confidence: 99%

How hyaluronan–protein complexes modulate the hyaluronidase activity: The model

Vincent¹,

Lenormand²

2009

Biophysical Chemistry

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Please cite this article as: Jean-Claude Vincent, Hélène Lenormand, How hyaluronanprotein complexes modulate the hyaluronidase activity: The model, Biophysical Chemistry (2009Chemistry ( ), doi: 10.1016Chemistry ( /j.bpc.2009 This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. Simulations performed by introducing experimental data produce a theoretical behaviour similar to the experimental one, including all the atypical phenomena observed : substratedependence, enzyme-dependence and protein-dependence of HAase. This shows that our assumptions are sufficient to explain the behaviour of the system and allows us to estimate unknown parameters and suggest new developments. A C C E P T E D

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Hyaluronan and hyaluronectin in the extracellular matrix of human brain tumour stroma

Cited by 163 publications

References 17 publications

Antisense inhibition of hyaluronan synthase-2 in human osteosarcoma cells inhibits hyaluronan retention and tumorigenicity

Antisense inhibition of hyaluronan synthase-2 in human osteosarcoma cells inhibits hyaluronan retention and tumorigenicity

Hyaluronan: fundamental principles and applications in cancer

How hyaluronan–protein complexes modulate the hyaluronidase activity: The model

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