Hyaluronic acid binding by human sperm indicates cellular maturity, viability, and unreacted acrosomal status

Huszár, Gábor; Özenci, Çiler Çelik; Çaylı, Sevil; Závaczki, Zoltán; Hansch, Eleonora; Vigue, Lynne

doi:10.1016/s0015-0282(03)00402-3

Cited by 248 publications

(227 citation statements)

References 34 publications

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“…It has been demonstrated that a method for invitro selection of mature spermatozoa based on sperm-HA binding results in spermatozoa for ICSI with low incidence of aneuploidies and DNA damage [1][2][3][4][5]. Since the chromosomal status and the chromatin integrity are not predictable by the observation of sperm dimension and shape [12] when performing conventional ICSI, the injection of aneuploid spermatozoa may generate chromosome aberrations in ICSI offspring [13,14]; likewise, oocyte fertilization with spermatozoa with damaged DNA may lead to an increased risk of pregnancy loss [15].…”

Section: Discussionmentioning

confidence: 99%

“…In fact, only mature spermatozoa which have extruded their specific receptors to bind to and digest HA can reach the oocyte and fertilize it. HA's role as "physiologic selector" is now well recognized also in vitro: it has been demonstrated that spermatozoa able to bind HA in vitro are those that have completed plasma membrane remodelling, cytoplasmic extrusion and nuclear maturation [1][2][3]. Furthermore, these HA-bound spermatozoa show low chromosomal aneuploidies and DNA fragmentation, and good nuclear morphology [4,5].…”

Section: Introductionmentioning

confidence: 99%

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Efficiency of hyaluronic acid (HA) sperm selection

Parmegiani

Cognigni

Ciampaglia

et al. 2009

J Assist Reprod Genet

117

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Efficiency of hyaluronic acid (HA) sperm selection

Parmegiani

Cognigni

Ciampaglia

et al. 2009

J Assist Reprod Genet

117

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“…They have shown that sperm creatine kinase (CK) and testisexpressed heat shock protein (HspA2) concentrations correlated with sperm maturity and had a predictive value for pregnancy [11,12]. During spermiogenesis binding sites for hyaluronic acid (HA) develop in the plasma membrane.…”

Section: Introductionmentioning

confidence: 99%

The role of hyaluronic acid binding assay in choosing the fertilization method for patients undergoing IVF for unexplained infertility

Kovács

Kováts

Sajgo

et al. 2010

J Assist Reprod Genet

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Purpose Patients with unexplained infertility may have fertilization problems. Split fertilization (ICSI and conventional IVF on sibling oocytes) is often used to avoid poor fertilization. Our aim was to assess the ability of hyaluronic acid binding (HA-binding) assay to predict spontaneous fertilization during IVF. Methods Prospective, blinded, controlled trial. Patients undergoing their first IVF cycle for unexplained infertility were eligible. Split fertilization was used. IVF and ICSI fertilization rates and embryo development based on 3 HAbinding cut-offs (< 60%; 60-80%; >80%) were compared. Results ICSI fertilization was higher than IVF, but none of the HA-binding cut-off levels predicted those cases where IVF was less effective, therefore ICSI only would have lead to improved outcome. Embryo development and morphology were similar in all cut-off groups. Conclusions HA-binding did not predict spontaneous fertilization in patients with unexplained infertility undergoing IVF treatment. When it was used for "screening" it did not help to select the method of fertilization.

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“…Noting an association between the presence of sperm membrane HA receptors and various upstream features of sperm maturity, Huszar et al 43 hypothesized that mature sperm would selectively bind to HA. They also surmised that sperm of diminished maturity have lower levels of the 70-kd testis-expressed chaperone (HspA2), increased chromosomal aberrations, and, because they have not undergone membrane remodeling, are unable to bind to solid-state HA.…”

Section: Reactive Oxygen Speciesmentioning

confidence: 99%

“…They also surmised that sperm of diminished maturity have lower levels of the 70-kd testis-expressed chaperone (HspA2), increased chromosomal aberrations, and, because they have not undergone membrane remodeling, are unable to bind to solid-state HA. 43 Lastly, based on these attributes, Huszar et al postulated that HA binding would facilitate the selection of individual mature sperm with lower levels of chromosomal aneuploidy. 44 In addition, Parmegiani et al suggest that HA-selected ICSI sperm produce equivalent fertilization rates and good quality embryos compared with visually selected sperm, but may improve live baby rates substantially by decreasing miscarriage rates.…”

Section: Reactive Oxygen Speciesmentioning

confidence: 99%

An Assessment of New Sperm Tests for Male Infertility

Natali

Turek

2011

Urology

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The routine semen analysis, although used for more than 50 years, fails to accurately distinguish between fertile and infertile men. As a consequence, many tests of sperm function (TSF) have been developed. This review discusses both older and newer diagnostic TSF. It outlines the principles underlying each assay and reviews aggregate clinical data to determine its current relevance and utility. It concludes that the relevance of many older TSF is questionable, with the wide acceptance of intracytoplasmic sperm injection (ICSI). Newer TSF have the potential to deliver more clinically relevant information but require more extensive study to better understand their predictive role in the ICSI era. UROLOGY 77: 1027-1034, 2011. © 2011 Elsevier Inc. All rights reserved.I n addition to the medical history and physical examination, the conventional semen analysis has been an essential laboratory test for the evaluation of male fertility for at least 50 years. However, the idea that fertility can be defined by threshold values of semen parameters is a concept that is fundamentally flawed. 1 Although not a true measure of fertility, the semen analysis, if abnormal, suggests that the probability of achieving fertility is lower than normal. 2 Because of biological variability, two semen analyses are generally needed, performed with two to three days of sexual abstinence and evaluated in a standardized fashion. 2 Recognized normal values for semen parameters are given in Table 1 in agreement with the latest recommendations by the World Health Organization (WHO). 2 Traditionally, (except for the latest WHO recommendations), these references are derived by expert consensus and not by prospective clinical trials and thus their true relationship to male fertility is unclear. In addition, the definition of what constitutes "normal" semen parameters is constantly challenged. A meta-analysis of 29 US studies of semen quality from 9612 fertile, or presumably fertile, men suggested that a sperm concentration of 98 million/mL is normal. 3 Although sperm motility is considered the "best" predictor of fertility, normal sperm motility ranges from 53% to 62%. 4 Thus, simply deriving "normal" semen parameters has been a prohibitively lengthy and inconclusive process to date.Finally, other biological variables affect the clinical utility of the semen analysis. In addition to wide intraindividual variation, seasonal 5 and geographic variation 4 further complicate the performance of the semen analysis as a fertility measure. As examples, within-subject, interejaculate coefficients of variation for sperm concentration and motility are estimated at 44.7% and 15% in one study. 1 Thus, even with excellent quality control, wide biological variation in semen quality profoundly challenges the notion that the conventional semen analysis can accurately assess male fertility. OLDER ADJUNCTIVE SPERM TESTSBecause of the need to more precisely characterize normal fertile semen, adjunctive semen testing has become popular (Figure 1). The concept behind de...

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Hyaluronic acid binding by human sperm indicates cellular maturity, viability, and unreacted acrosomal status

Cited by 248 publications

References 34 publications

Efficiency of hyaluronic acid (HA) sperm selection

Efficiency of hyaluronic acid (HA) sperm selection

The role of hyaluronic acid binding assay in choosing the fertilization method for patients undergoing IVF for unexplained infertility

An Assessment of New Sperm Tests for Male Infertility

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