Hybrid adaptive and computational light-sheet fluorescence microscopy

Abstract

The light-sheet fluorescence microscopy is an excellent tool for the investigation of large three dimensional microscopy samples at the cellular level, however, the ability to resolve features is strongly affected by the presence of scattering and aberrations. These effects are two fold in light-sheet microscopy, as the illumination path providing the optical sectioning and the fluorescence detection path are both affected by the aberrations in different ways. To overcome these difficulties, we have developed hybrid adaptive optical and computational microscopy techniques to remove the effect of the aberrations in both the excitation and the fluorescence paths of these microscopes.

Adaptive optics for optical microscopy [Invited]

“…Phase diversity is closely related to blind deconvolution approaches in which both the object and the aberrations are calculated from a series of images. Various blind deconvolution algorithms have been used in microscopy although typically instead of, rather than with, adaptive optics [ 170 , 171 ]. In one early approach to correcting aberrations with deconvolution, the aberrations were calculated from a refractive index map derived from DIC images [ 172 ].…”