Hybrid Chitosan/CaO-Based Nanocomposites Doped with Plant Extracts from Azadirachta indica and Melia azedarach: Evaluation of Antibacterial and Antibiofilm Activities

Paunova-Krasteva, Tsvetelina; Hemdan, Bahaa A.; Dimitrova, Petya D.; Damyanova, Tsvetozara; El-Feky, Amal M.; Elbatanony, Marwa M.; Stoitsova, Stoyanka; El‐Liethy, Mohamed Azab; El‐Taweel, Gamila E.; Nahrawy, Amany M. El

doi:10.1007/s12668-022-01047-0

Cited by 22 publications

(11 citation statements)

References 60 publications

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“…The stain was then dissolved in acetic acid (33%) and the readings were taken at 570 nm using Bio Rad microplate reader. The calculation in percentage of bacterial biofilm inhibition was performed using the following formula [ 39 , 62 ].

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Section: Methodsmentioning

confidence: 99%

Antibacterial and Antibiofilm Activity of Ficus carica-Mediated Calcium Oxide (CaONPs) Phyto-Nanoparticles

et al. 2023

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The significance of nanomaterials in biomedicines served as the inspiration for the design of this study. In this particular investigation, we carried out the biosynthesis of calcium oxide nanoparticles (CaONPs) by employing a green-chemistry strategy and making use of an extract of Ficus carica (an edible fruit) as a capping and reducing agent. There is a dire need for new antimicrobial agents due to the alarming rise in antibiotic resistance. Nanoparticles’ diverse antibacterial properties suggest that they might be standard alternatives to antimicrobial drugs in the future. We describe herein the use of a Ficus carica extract as a capping and reducing agent in the phyto-mediated synthesis of CaONPs for the evaluation of their antimicrobial properties. The phyto-mediated synthesis of NPs is considered a reliable approach due to its high yield, stability, non-toxicity, cost-effectiveness and eco-friendliness. The CaONPs were physiochemically characterized by UV-visible spectroscopy, energy-dispersive X-ray (EDX), scanning-electron microscopy (SEM), X-ray diffraction (XRD), and Fourier-transform infrared spectroscopy (FTIR). The biological synthesis of the calcium oxide nanoparticles revealed a characteristic surface plasmon resonance peak (SPR) at 360 nm in UV-Vis spectroscopy, which clearly revealed the successful reduction of the Ca2+ ions to Ca0 nanoparticles. The characteristic FTIR peak seen at 767 cm−1 corresponded to Ca-O bond stretching and, thus, confirmed the biosynthesis of the CaONPs, while the scanning-electron micrographs revealed near-CaO aggregates with an average diameter of 84.87 ± 2.0 nm. The antibacterial and anti-biofilm analysis of the CaONPs showed inhibition of bacteria in the following order: P. aeruginosa (28 ± 1.0) > S. aureus (23 ± 0.3) > K. pneumoniae (18 ± 0.9) > P. vulgaris (13 ± 1.6) > E. coli (11 ± 0.5) mm. The CaONPs were shown to considerably inhibit biofilm formation, providing strong evidence for their major antibacterial activity. It is concluded that this straightforward environmentally friendly method is capable of synthesizing stable and effective CaONPs. The therapeutic value of CaONPs is indicated by their potential as a antibacterial and antibiofilm agents in future medications.

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…”

Section: Methodsmentioning

confidence: 99%

Antibacterial and Antibiofilm Activity of Ficus carica-Mediated Calcium Oxide (CaONPs) Phyto-Nanoparticles

et al. 2023

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“…Consequently, this strategy aims to control and eliminate the formed biofilm [ 68 , 69 ]. Conventional agents like antibiotics are commonly used, but escalating antibiotic resistance has led to a quest for substances with antibacterial or antibiofilm effects [ 70 , 71 , 72 , 73 ]. Unfortunately, the use of antibiotics has become a risk factor due to growing antibiotic resistance, underscoring the urgent need to discover more promising approaches to combating multidrug resistance.…”

Section: Therapeutic Strategies To Combat Bacterial Biofilmsmentioning

confidence: 99%

An Overview of Biofilm-Associated Infections and the Role of Phytochemicals and Nanomaterials in Their Control and Prevention

Damyanova,

Dimitrova,

Borisova

et al. 2024

Pharmaceutics

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Biofilm formation is considered one of the primary virulence mechanisms in Gram-positive and Gram-negative pathogenic species, particularly those responsible for chronic infections and promoting bacterial survival within the host. In recent years, there has been a growing interest in discovering new compounds capable of inhibiting biofilm formation. This is considered a promising antivirulence strategy that could potentially overcome antibiotic resistance issues. Effective antibiofilm agents should possess distinctive properties. They should be structurally unique, enable easy entry into cells, influence quorum sensing signaling, and synergize with other antibacterial agents. Many of these properties are found in both natural systems that are isolated from plants and in synthetic systems like nanoparticles and nanocomposites. In this review, we discuss the clinical nature of biofilm-associated infections and some of the mechanisms associated with their antibiotic tolerance. We focus on the advantages and efficacy of various natural and synthetic compounds as a new therapeutic approach to control bacterial biofilms and address multidrug resistance in bacteria.

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“…The antibacterial effect of CaONPs against Staphylococcus epidermidis, E coil, and Candida albicans growth was verified by two established measures: minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) 37 . A comparative study of the MIC, MFC, and MBC values of different microbes was presented in Table 2.…”

Section: Determination Of Mic Mbc Mfc and Mic Index Of Caonpsmentioning

confidence: 99%

The dual activity of CaONPs as a cancer treatment substance and at the same time resistance to harmful microbes

Awaad,

Olama,

El-Subruiti

et al. 2023

Sci Rep

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Nanotechnology holds significant promise for the development of novel and necessary products that enhance human health. Pharmacology and nanotechnology have contributed to developing advanced and highly effective drugs for cancer treatment and combating microbial infections. The microbiological effectiveness against the variety of examined microorganisms was assessed using the time killer curve, scanning electron microscopy (SEM), MIC techniques, and the agar well diffusion method. SEM was utilized to enhance the analysis of the mechanisms underlying the bio-interface interaction and intracellular localization of calcium oxide nanoparticles (CaONPs). The MTT test was used to examine the cytotoxicity of CaONP anticancer activity in various cancer cells, including colon, breast, and hepatic cells. The efficacy of CaONPs as an anticancer medication was elucidated by analyzing the gene expression of both treated and untreated cancer cells. MIC and MBC of CaONPs against Escherichia coli and Staphylococcus epidermidis were 150, 150, 150, and 200 µg/ml, respectively. The MIC and MFC of CaONPs against Candida albicans were 200 µg/ml and 250 µg/ml, respectively. The IC50 values of various CaONPs vary depending on the type of cancer cells. The gene expression analysis of breast cancer cells undergoing treatment revealed the identification of several cancer-controlling genes, namely BAX, BCL2, P53, TERT, KRAS1, KRAS2, and RB1. The study demonstrated the notable antibacterial efficacy of CaONPs, highlighting their potential as cancer therapies.

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Hybrid Chitosan/CaO-Based Nanocomposites Doped with Plant Extracts from Azadirachta indica and Melia azedarach: Evaluation of Antibacterial and Antibiofilm Activities

Cited by 22 publications

References 60 publications

Antibacterial and Antibiofilm Activity of Ficus carica-Mediated Calcium Oxide (CaONPs) Phyto-Nanoparticles

Antibacterial and Antibiofilm Activity of Ficus carica-Mediated Calcium Oxide (CaONPs) Phyto-Nanoparticles

An Overview of Biofilm-Associated Infections and the Role of Phytochemicals and Nanomaterials in Their Control and Prevention

The dual activity of CaONPs as a cancer treatment substance and at the same time resistance to harmful microbes

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