Hybrid performance and AFLP- based genetic similarity in faba bean

Abstract

Successful prediction of heterosis and performance of F 1 -hybrids from the genetic similarity of their parents based on molecular markers has been reported in several crops and can be very helpful in hybrid breeding. The relationship between genetic similarities based on amplified fragment length polymorphism (AFLP) of 18 European faba bean lines and their hybrid performance and heterosis was investigated. Parental lines, 62 F 1 -hybrids and their F 2 -progenies were evaluated in field trials in four environments in Germany for seed yield, 1,000-seed weight and plant height. Results clearly demonstrated a stable superiority of the hybrids over their inbred parents and elite check cultivars, and showed a marked and varying amount of heterosis. Parental seed yield and F 2 -hybrid yield were promising as predictors for F 1 -hybrids. AFLP analysis of the 18 inbred lines using 26 EcoRI/MseI primer combinations resulted in 1202 polymorphic fragments. Cluster analysis based on genetic similarity estimates unambiguously identified pedigree-related inbred lines. No clear separation of the 18 inbred lines into subgroups was detected. Correlation coefficients between genetic similarity estimates and either heterosis or F 1 -hybrid performance were small and not useful. Also correlations between specific genetic similarity and specific combining ability were too small for all traits to be of predictive value. Results showed that AFLP-based genetic similarities are not useful to predict the performance of hybrids or heterosis within the elite European faba bean gene pool.

Heterosis and path analysis for grain yield and chocolate spot disease resistance in faba bean (Vicia faba L.)

“…Similarly, the significant mean squares due to heterosis, variety heterosis and specific heterosis for chocolate spot disease resistance and grain yield suggested the potential of the parents for heterosis. These results confirm those obtained by Zeid et al (2004) and Alghamdi (2009).…”

“…The highest heterosis for grain yield of 162.3% in this study was higher than reported in other studies, for instance 129.9% by Alghamdi (2009), 119% by (Zeid et al, 2004). The high heterosis for grain yield was detected in crosses made between the introduced inbred lines (ILB-4726, ILB-938 and BPL-710) and locally released improved varieties (NC58, Kasa, Bulga-70 and CS-20-DK).…”

“…Heterosis over mid and better parents were reported in faba bean crosses (Alghamdi, 2009). Zeid et al (2004) also reported a stable superiority of the hybrids in faba bean over their inbred parents with marked and varying amounts of heterosis.…”

Conventional and Molecular Breeding Tools for Accelerating Genetic Gain in Faba Bean (Vicia Faba L.)

“…Hybrid vigor (heterosis) is pronounced in faba bean. Heterozygous F1-hybrids exceed the yield of their homozygous parents by 40 to 70% (Zeid et al, 2004;Dieckmann and Link, 2010). Early approaches to hybrid breeding using cytoplasmic male sterility (CMS) system in faba bean trace back to David Bond in Cambridge, UK in 1957 and Pierre Berthelem in Rennes, France in 1967 (Pfeiffer et al, 1993;Link et al, 1997).…”

Genetic and Phenotypic Variation Among Faba Bean Landraces and Cultivars

“…The AFLP markers were used to investigate the relationship between genetic similarities of 18 European faba bean lines and their hybrid performance and heterosis (Zeid et al, 2004). No previous work has been done using SSR markers to study genetic diversity in faba bean.…”