Hydration-aggregation pretreatment for drastically improving esterification activity of commercial lipases in non-aqueous media

Katayama, Maho; Kuroiwa, Takashi; Suzuno, Kenya; Igusa, Ayumi; Matsui, Toru; Kanazawa, Akihiko

doi:10.1016/j.enzmictec.2017.06.007

Cited by 16 publications

(5 citation statements)

References 54 publications

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“…Moreover, the presence of certain amounts of water in the solvent can induce lipase aggregation, and this has also been reported as a lipase activation strategy. 50 On the other hand, if the amount of water in the reaction medium is too high, enzyme denaturation may occur, as well as enzyme inhibition because of excess water when water is a reaction product. 51 Water content, expressed as water activity (a w ), is then a very important parameter to consider in lipase-catalyzed reactions in hydrophobic DES not only because it can change the solvent characteristics but also because it is intimately related with the conformational freedom of the enzyme, which is directly related with its optimal activity.…”

Section: ■ Results and Discussionmentioning

confidence: 99%

“…The hydration level that confers maximum activity depends on the enzyme. Moreover, the presence of certain amounts of water in the solvent can induce lipase aggregation, and this has also been reported as a lipase activation strategy . On the other hand, if the amount of water in the reaction medium is too high, enzyme denaturation may occur, as well as enzyme inhibition because of excess water when water is a reaction product …”

Section: Resultsmentioning

confidence: 99%

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Deep Eutectic Solvents for Enzymatic Esterification of Racemic Menthol

Craveiro

Meneses

Durazzo

et al. 2019

ACS Sustainable Chem. Eng.

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Enzymatic esterification is an effective route that can contribute for the separation of racemic (rac) mixtures and is thus of great importance for the pharmaceutical, food, and cosmetic industries. All the processes involved in this methodology should be environmentally friendly, biocompatible, fast, and cost-effective. The use of deep eutectic solvents (DESs) can comply with all these metrics. In this work, we report on the use of DESs in the enzymatic esterification of menthol, one of the largest traded flavors and aromas worldwide. Solid rac-menthol was mixed with solid lauric acid at different molar ratios, yielding a DES liquid at room temperature or slightly above. Rac-menthol and lauric acid were the DES components and also the esterification reaction substrates. The DES reacted promptly when Candida rugosa lipase was added, and the conversions of rac-menthol reached up to 44% after 3 h of reaction and an enantiomeric excess of the product of 62%. Enzyme activity was dependent on the molar ratio of the substrates as well as on the water activity in the DES. The use of DES combined with enzymes paves the way for efficient and more sustainable biocatalytic processes.

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Section: ■ Results and Discussionmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Deep Eutectic Solvents for Enzymatic Esterification of Racemic Menthol

Craveiro

Meneses

Durazzo

et al. 2019

ACS Sustainable Chem. Eng.

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“…However, by using the phytosterol as acyl receptor, highest PEs yield of 17.1% was spotted from the CRL without the molecular sieves. This phenomenon was related to the characteristics of CRL, which required a certain amount of water to maintain its catalytic activity (Grochulski, Li, Schrag, & Cygler, 1994;Katayama, Kuroiwa, Suzuno, Igusa, Matsui, & Kanazawa, 2017;Li et al, 2018). Therefore, the main reaction mechanisms of these two acyl receptors catalyzed by different lipases were summarized.…”

Section: Screening Of Acyl Receptors and Enzymesmentioning

confidence: 99%

Deacidification of high-acid rice bran oil by the tandem continuous-flow enzymatic reactors

Zhang

Živković

et al. 2022

Food Chemistry

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“…Lipases are enzymes belonging to the hydrolase group, capable of catalyzing both hydrolysis and esterification reactions ( 23 – 25 ). A lower moisture content of reaction system provides a greater possibility for lipases to catalyze esterification reaction than that of hydrolysis reaction.…”

Section: Enzymes For Mlm Structured Lipid Synthesis: Types Ans Sourcesmentioning

confidence: 99%

Lipase-Catalyzed Interesterification for the Synthesis of Medium-Long-Medium (MLM) Structured Lipids

Utama

Sitanggang

Adawiyah

et al. 2019

Food technol. biotechnol. (Online)

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SUMMARYMedium-long-medium (MLM) structured lipids typically contain medium-chain fatty acids (C6-C12) at sn-1,3 and long-chain fatty acids (C14-C24) at sn-2 positions. They have reduced calories and are suitable for the control of obesity, lipid malabsorption and other metabolic disorders. This review focuses on the synthesis of MLM lipids by the enzymatic interesterification. It gives detailed description of biocatalysts, substrates, reactors and synthesis methods, and discusses the use of MLM lipids in food products. The information provided in this review can be considered as the current state-of-the art for developing a future strategy for the synthesis of MLM structured lipids.

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Hydration-aggregation pretreatment for drastically improving esterification activity of commercial lipases in non-aqueous media

Cited by 16 publications

References 54 publications

Deep Eutectic Solvents for Enzymatic Esterification of Racemic Menthol

Deep Eutectic Solvents for Enzymatic Esterification of Racemic Menthol

Deacidification of high-acid rice bran oil by the tandem continuous-flow enzymatic reactors

Lipase-Catalyzed Interesterification for the Synthesis of Medium-Long-Medium (MLM) Structured Lipids

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