This research aimed to examine the hydration knowledge level, attitudes and behaviors of athletes in different branches. A total of 553 athletes, 123 (22.2%) females and 430 (77.8%) males, were included in the research conducted with the screening model. The data was obtained by using the "Personal Information Form" and the "Hydration Knowledge, Attitude and Behavior Survey". T-test for independent groups and one-way analysis of variance were used in the analysis of data. When the research findings were examined, it was determined that there were statistically significant differences in athletes' attitudes and behavior according to gender, attitude according to sport type, knowledge and behavior according to age groups, knowledge, attitude and behavior according to sports age, and knowledge and behavior according to their educational status. As a result, it was determined that the athletes' general knowledge scores were at a high level, while their attitude and behavior scores were at a medium level.