Hydraulic calcium silicate-based root canal sealers mitigate proinflammatory cytokine synthesis and promote osteogenesis in vitro

Alchawoosh, Aseel; Hashimoto, Kentaro; Kawashima, Nobuyuki; Noda, Sonoko; Nozaki, Kosuke; Okiji, Takashi

doi:10.1016/j.jds.2022.12.019

Cited by 8 publications

(5 citation statements)

References 43 publications

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“…IL-6 plays an integral role in bone remodeling, as well as in the activation and differentiation of immune cells and osteoclasts. Previous research has established a link between elevated IL-6 levels and the occurrence of symptomatic and active endodontic lesions [ 28 ]. Moreover, IL-8 has been shown to promote the inflammatory response and the progression of such lesions.…”

Section: Discussionmentioning

confidence: 99%

Biocompatibility, bioactivity and immunomodulatory properties of three calcium silicate-based sealers: an in vitro study on hPDLSCs

Mora,

García-Bernal,

Rodríguez-Lozano

et al. 2024

Clin Oral Invest

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Objectives To assess the biocompatibility, bioactivity, and immunomodulatory properties of three new calcium silicate cement-based sealers: Ceraseal (CS), Totalfill BC Sealer (TFbc) and WellRoot ST (WR-ST) on human periodontal ligament stem cells (hPDLSCs). Materials and methods HPDLSCs were isolated from extracted third molars from healthy patients. Eluates (1:1, 1:2, and 1:4 ratio) and sample discs of CS, TFbc and WR-ST after setting were prepared. A series of assays were performed: cell characterization, cell metabolic activity (MTT assay) cell attachment and morphology (SEM assay), cell migration (wound-healing assay), cytoskeleton organization (phaloidin-based assay); IL-6 and IL-8 release (ELISA); differentiation marker expression (RT-qPCR assay), and cell mineralization (Alizarin Red S staining). HPDLSCs cultured in unconditioned (negative control) or osteogenic (positive control) culture media were used as a comparison. Statistical significance was established at p < 0.05. Results All the tested sealers exhibited similar results in the cytocompatibility assays (cell metabolic activity, migration, attachment, morphology, and cytoskeleton organization) compared with a negative control group. CS and TFbc exhibited an upregulation of at least one osteo/cementogenic marker compared to the negative and positive control groups. CS and TFbc also showed a significantly higher calcified nodule formation than the negative and positive control groups. Both the marker expression and calcified nodule formation were significantly higher in CS-treated cells than TFbc treated cells. WR-ST exhibited similar results to the control group. CS and TFbc-treated cells exhibited a significant downregulation of IL-6 after 72 h of culture compared to the negative control group (p < 0.05). Conclusion All the tested sealers exhibited an adequate cytocompatibility. CS significantly enhances cell differentiation by upregulating the expression of key genes associated with bone and cementum formation. Additionally, CS was observed to facilitate the mineralization of the extracellular matrix effectively. In contrast, the effects of TFbc and WR-ST on these processes were less pronounced compared to CS. Furthermore, both CS and TFbc exhibited an anti-inflammatory potential, contributing to their potential therapeutic benefits in regenerative endodontics. Clinical relevance This is the first study to compare the biological properties and immunomodulatory potential of Ceraseal, Totalfill BC Sealer, and WellRoot ST. The results act as supporting evidence for their use in root canal treatment.

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Section: Discussionmentioning

confidence: 99%

Biocompatibility, bioactivity and immunomodulatory properties of three calcium silicate-based sealers: an in vitro study on hPDLSCs

Mora,

García-Bernal,

Rodríguez-Lozano

et al. 2024

Clin Oral Invest

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“…Stem cells from the elderly, similar to cells from the young, exhibit a reaction to these signals [ 87 , 89 ]. In contrast, a study by Tiantian Wu et al [ 56 ] demonstrated that the use of calcium silicate cement significantly influenced cell viability, the high alkalinity of the calcium silicate-based sealer media can up-regulate alkaline phosphatase activity and enhance mineralization [ 56 , [90] , [91] , [92] , [93] , [94] ].…”

Section: Discussionmentioning

confidence: 99%

Advances and challenges in regenerative dentistry: A systematic review of calcium phosphate and silicate-based materials on human dental pulp stem cells

Christie,

Musri,

Djustiana

et al. 2023

Materials Today Bio

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“…EndoSequence BC Sealer (a first-generation premixed CSCS; Brasseler, Savannah, GA, USA; ES) contains monobasic calcium phosphate, which reacts with calcium hydroxide and promotes the nucleation of carbonated apatites [15]. Premixed CSCSs are highly biocompatible [9,16,17] and capable of inducing favorable cellular responses, including the promotion of osteogenic gene expression and mineralized nodule formation in osteoblastic cells [16,17] and the suppression of proinflammatory cytokine expression in macrophages [17]. These findings provide a biological basis for the clinical application of these sealers.…”

Section: Introductionmentioning

confidence: 99%

Heat-Induced Changes in the Physical Properties of a New Premixed Calcium Silicate-Containing Root Canal Sealer: An In Vitro Study

Ito,

Watanabe,

Kouno

et al. 2024

Dentistry Journal

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This study aimed to examine how heating affects the physical properties of a newly developed premixed calcium silicate-containing sealer (AH Plus Bioceramic Sealer; AHB), in comparison with EndoSequence BC Sealer (ES), AH Plus Jet (AH), and Pulp Canal Sealer. The setting time, flow, and film thickness were tested with or without heating at 100 °C for 30 or 60 s, in accordance with ISO6876:2012 standards. Ultrastructural and elemental analyses were performed with scanning electron microscopy (SEM) and energy-dispersive X-ray spectroscopy (EDS). Data were analyzed using one-way analysis of variance (ANOVA) with a Tukey post hoc test (α = 0.05). All sealers exhibited significantly shorter setting time and flow after heating at 100 °C for 30 and 60 s (p < 0.05). After heating, AHB showed a significantly higher film thickness compared to that of the other materials (p < 0.05). None of the tested properties of heat-applied AHB and ES met ISO standards, except the setting time in ES. The SEM/EDS results for AHB and ES were not affected by heating. The detected changes in physical properties can negatively impact the performance of premixed calcium silicate-containing sealers, particularly AHB, when warm vertical compaction is employed.

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Hydraulic calcium silicate-based root canal sealers mitigate proinflammatory cytokine synthesis and promote osteogenesis in vitro

Cited by 8 publications

References 43 publications

Biocompatibility, bioactivity and immunomodulatory properties of three calcium silicate-based sealers: an in vitro study on hPDLSCs

Biocompatibility, bioactivity and immunomodulatory properties of three calcium silicate-based sealers: an in vitro study on hPDLSCs

Advances and challenges in regenerative dentistry: A systematic review of calcium phosphate and silicate-based materials on human dental pulp stem cells

Heat-Induced Changes in the Physical Properties of a New Premixed Calcium Silicate-Containing Root Canal Sealer: An In Vitro Study

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